CC BY-NC-ND 4.0 · Laryngorhinootologie 2018; 97(S 02): S182-S183
DOI: 10.1055/s-0038-1640345
Abstracts
Otologie: Otology
Georg Thieme Verlag KG Stuttgart · New York

DNA-functionalized calcium phosphate nanoparticles coated on a cochlear implant electrode

S Hansen
1  HNO-Klinik, Universitätsklinikum Essen, Essen
,
R Weller
1  HNO-Klinik, Universitätsklinikum Essen, Essen
,
M Epple
2  Institut für Anorganische Chemie, Universität Duisburg-Essen, Essen
,
S Lang
1  HNO-Klinik, Universitätsklinikum Essen, Essen
› Author Affiliations
Deutsche Forschungsgemeinschaft: HA 7395/3 – 1
Further Information
Priv.-Doz. Dr. Stefan Hansen
HNO-Klinik,
Hufelandstr. 55, 45147,
Essen

Publication History

Publication Date:
18 April 2018 (online)

 
 

    Introduction:

    So far, studies on a nanoparticle-mediated genetically active coating of a cochlear implant electrode are not existent. Such a coating of the electrode, which could be provided with a neurotrophin-encoding DNA, would provide a stable and controlled release over time of growth-promoting factors for the spiral ganglion cells. Because calcium phosphate nanoparticles constitute a DNA carrier system and also form a non-viral transfection system of surfaces, they can potentially be used as a biologically active coating of a cochlear implant electrode.

    Methods:

    The physical, chemical and biological properties such as transfection efficiency and cytotoxicity of the calcium phosphate nanoparticles were investigated. The aim was to evaluate the functionalized nanoparticles in a spiral ganglion cell culture. These were freshly taken from neonatal rat cochlea and prepared for a dissociated cell culture and an organotypic explant culture and then cocultivated with differently functionalized calcium phosphate nanoparticles.

    Results:

    The nanoparticles were mainly uptaken in the non-neuronal cells and less in the spiral ganglion cells themselves. Cytotoxic effects by the nanoparticles could not be observed for a culture period of up to one week.

    Conclusion:

    Calcium phosphate nanoparticles could be an interesting non-viral vector system for the transfection of genetic material into the inner ear. In particular, the possible coating of a cochlear implant electrode could thereby generate a long-term release of neurotrophic factors in the inner ear.


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    No conflict of interest has been declared by the author(s).

    Priv.-Doz. Dr. Stefan Hansen
    HNO-Klinik,
    Hufelandstr. 55, 45147,
    Essen