Subscribe to RSS
DOI: 10.1055/s-0038-1640863
Effects of polypoid tissue on peripheral lymphocytes in a co-culture system
T cell subpopulations in nasal polyps differ from peripheral lymphocytes in patients of CRSwNP. These T cells crucially determine the phenotype of nasal polyps. However, there is little known about the modulatory influence of the inflamed nasal mucosa on the phenotype of the T cells. The aim of this study was to analyze this interaction.
Tissue and blood samples were collected from 10 patients undergoing paranasal sinus surgery. Polypoid tissue was cultured under air-liquid-interface conditions. Afterwards CD3/CD28 activated PBMC of the same patients were added. After 3 days, lymphocytes were separated from co-culture and analyzed by multicolor flow cytometry. As control group, PBMC were cultured in the same setting without the co-cultured polypoid tissue. Additionally, cytokine expression of the polyp tissue was measured using a human Th1/Th2/Th17 antibody array.
There was a significantly higher amount of CD4+and CD8+ T cells in the co-cultured system than in PBMC alone. Terminal differentiated CD8+ T cells were increased, while central memory CD8+ T cells decreased in the co-culture. HLA-DR was significantly downregulated on co-cultured CD3+ lymphocytes. Conventional memory CD4+ T cells increased and resting regulatory T cells decreased in the co-cultured system. Cytokine analysis showed an expression of Il-6, GMCSF and MIP-3. All results showed significance.
In this study, a reproducible effect of nasal polypoid tissue on PBMC was shown. Interestingly, the significant downregulation of HLA-DR on CD3+ lymphocytes was confirmed in vitro. The inhibitory effect of the polypoid tissue on the activation of lymphocytes is a possible pathomechanism of this disease.
No conflict of interest has been declared by the author(s).
Publication History
Publication Date:
18 April 2018 (online)
© 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).
Georg Thieme Verlag KG
Stuttgart · New York