Preferential Disappearance of Aerobically Generated ATP from Platelets during Thrombin-induced Aggregation

Satoko Matsui; Yasuko Watanabe; Bonro Kobayashi

doi:10.1055/s-0038-1647712

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1974; 32(02/03): 441-456
DOI: 10.1055/s-0038-1647712

Original Article

Schattauer GmbH

Preferential Disappearance of Aerobically Generated ATP from Platelets during Thrombin-induced Aggregation

Satoko Matsui

¹Department of Physiological Chemistry, College of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo, Japan

,

Yasuko Watanabe

¹Department of Physiological Chemistry, College of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo, Japan

,

Bonro Kobayashi

¹Department of Physiological Chemistry, College of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo, Japan

› Author Affiliations

Abstract

Summary

Thrombin-induced nucleotide disappearance from rabbit platelets labelled with ³²P under oxidative and glycolytic conditions was observed. After adding thrombin to a platelet suspension previously incubated with ³²P in the presence of succinate or of glucose + KCN, radioactive ATP formed under the oxidative condition disappeared rapidly from cells and medium, while that formed under the glycolytic condition remained within aggregates. Since no significant difference was observed between the amounts of ATP generated during thrombin-induced aggregation in both systems, the preservation of radioactive ATP in aggregates under glycolytic conditions cannot be attributed to an increase in ATP generation through glycolysis during aggregation. Rather, it is conceivable that consumption of newly synthesized ATP is markedly stimulated under oxidative conditions. Sonic disruption of labelled platelets followed by subcellular fractionation revealed that ATP generated in both the oxidative and glycolytic systems was found in the soluble fraction of the cells. The possible presence of sub compartments in “the rapidly labelled pool” of ATP in the soluble fraction is discussed.

Full Text

References

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