Thromb Haemost 1975; 33(02): 226-229
DOI: 10.1055/s-0038-1647876
Original Article
Schattauer GmbH

Sodium Acetate Buffer

A Diluent of Choice in the Clot Lysis Time Technique
I. S Chohan1
1   Coagulation Laboratory, Referal and Research Centre, Army Hospital, Delhi Cant, India
2   Laboratorium Bloedstolling, Academisch Ziekenhuis St. Rafaël, Leuven, Belgium
,
J Vermylen2
1   Coagulation Laboratory, Referal and Research Centre, Army Hospital, Delhi Cant, India
2   Laboratorium Bloedstolling, Academisch Ziekenhuis St. Rafaël, Leuven, Belgium
,
I Singh1
1   Coagulation Laboratory, Referal and Research Centre, Army Hospital, Delhi Cant, India
2   Laboratorium Bloedstolling, Academisch Ziekenhuis St. Rafaël, Leuven, Belgium
,
K Balakrishkan1
1   Coagulation Laboratory, Referal and Research Centre, Army Hospital, Delhi Cant, India
2   Laboratorium Bloedstolling, Academisch Ziekenhuis St. Rafaël, Leuven, Belgium
,
M Verstraete2
1   Coagulation Laboratory, Referal and Research Centre, Army Hospital, Delhi Cant, India
2   Laboratorium Bloedstolling, Academisch Ziekenhuis St. Rafaël, Leuven, Belgium
› Author Affiliations
Further Information

Publication History

Received 16 June 1974

Accepted 20 August 1974

Publication Date:
02 July 2018 (online)

Summary

Sodium acetate buffer, 0.12 M, pH 7.4 as a diluent in the low temperature technique of dilute clot lysis time, is more effective in accelerating the velocity of lysis than phosphate buffer of similar pH and molarity. A uniform shape of the clot is maintained throughout the digestion in sodium acetate buffer and the end point of lysis is characteristically marked by an abrupt and sharply defined disintegration. Sodium acetate buffer can be employed advantageously in this technique not only to improve the observation but also to shorten the lysis times.

1 Present address: Directorate General, Armed Forces Medical Services, DHQ, New Delhi-I, India.


2 Academisch Ziekenhuis Sint Rafaël, Katholieke Universitteit Leuven, 3000 Leuven, Belgium.


 
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