Studies on the Role of Fibrinolysis and Fibrinogen Degradation Products (FDP) in Analgesic Action of Morphine

Summary

The role of fibrinolysis and FDP in the analgesic action of morphine in mice and rats was studied. It was shown that during activation of blood fibrinolysis, both the accumulation of morphine in the brain tissue of rats and the clinical effect of this drug were increased. Similar results were observed after morphine given simultaneously with FDP obtained in vitro. The data from the analysis of FDP carried out on Sephadex G-25 Fine columns suggest that only FDP of molecular weight of about 10,000 potentiate the action of morphine; smaller peptides decreased the action of this drug.

• References

• 1 Beck E. A, and Jackson D. P. 1966; Studies on the degradation of human fibrinogen by plasmin and trypsin. Thrombosis et Diathesis Haemorrhagica 16: 526.
• 2 Bielawiec M. 1964; Prolongation of increased fibrinolysis with activators present in the organism. Polski Tygodnik Lekarski 19: 581.
• 3 Buluk K, Januszko T, Olbromski J, and Chmielewski J. 1962; O aktywacji fibrynolizy. Polskie Archiwum Medycyny Wewnetrznej 32: 769.
• 4 Buluk K, Małofiejew M, and Czokało M. 1966; Unknown properties of the products of plasmin - degradation of fibrinogen and fibrin. Bulletin de l’Académie Polonaise des Sciences; Série des Sciences Biologiques 14: 193.
• 5 Cash J. D. 1966; Effect of moderate exercise on the fibrinolytic system in normal young men and women. British Medical Journal 2: 502.
• 6 Copley A. L. 1968; On the physiological role of fibrinogen and fibrin. Postepy Biochemii 14: 343.
• 7 Danysz A, Buczko Wł, and Wiśniewski K. 1970; Studies on the participation of angiotensin in transport and metabolic of isonicotinic acid hydrazide (INH). Acta Physiologica Polonica 21: 811.
• 8 Danysz A, Buczko Wł, and Wiśniewski K. 1969; The influence of somatotropin on the chlorpromazine distribution in rat tissues. Agressologie 10: 391.
• 9 Danysz A, and Wiśniewski K. 1965; The influence of insulin on drug passage into the tissues. Archives Internationales de Pharmacodynamie et de Terapie 158: 30.
• 10 Danysz A, Wiśniewski K, Buczko Wł, and Zdrodowska B. 1967; The influence of posterior pituitary hormones on chlorpromazine action and penetration across cell membranes. Disser-tationes Pharmaceuticae et Pharmacologicae 19: 235.
• 11 Fletcher A. P, Alkjaersig N, Fischer S, and Sherry S. 1966; The proteolysis of fibrinogen by plasmin : The identification of thrombin clottable fibrinogen derivatives which polymerize abnormally. Journal of Laboratory and Clinical Medicine 68: 780.
• 12 Kekwick R. A, MacKay M. E, Nance M. H, and Record B. R. 1955; The purification of human fibrinogen. Biochemical Journal 60: 671.
• 13 Kotschy M, Kotschy A, and Krauze K. 1961; Odczyn fibrynolityczny w elektrowstrząsie. Postȩpy Higieny i Medycyny Doświadczalnej 15: 229.
• 14 Kowałski E. 1968; Fibrinogen derivatives and their biologic activities. Seminars in Hematology 5: 45.
• 15 Kowałski E, Kopeć M, and Niewiarowski S. 1959; An evaluation of the euglobin method for the determination of fibrinolysis. Journal of Clinical Pathology 12: 215.
• 16 Lake K. 1965; Enzymatic effects of thrombin. Federation Proceedings 24: 795.
• 17 Małofiejew M. 1969; On the biological properties of some fibrinogen degradation products (FDP). Roczniki Akademii Medycznej im. J. Marchlewskiego w Bialymstoku. Suppl 23: 1.
• 18 Małofiejew M, and Buluk K. 1968; Effect of products of plasmin degradation of fibrinogen on the permeability of capillaries. Polski Tygodnik Lekarski 17: 619.
• 19 Merskey C, Johnson A. J, Kleiner G. J, and Wohl H. 1967; The defibrination syndrome: Clinical features and laboratory diagnosis. British Journal of Haematology 13: 528.
• 20 Merskey C, Kleiner J. G, and Johnson A. J. 1966; Quantitative estimation of split products of fibrinogen in human serum. Relation to diagnosis and treatment. Blood; Journal of Hematology 28: 1.
• 21 Marder V. J. 1971 Identification and purification of fibrinogen degradation products produced by plasmin: considerations on the structure of fibrinogen. In: Verstraete M, Vermylen J, and Donati M. B. (Ed.) Fibrinogen Degradation Products. International Workshop held from July 22nd to 25th 1970, Leuven (Belgium). Scandinavian Journal of Haematology. Suppl. 13, p.21.
• 22 Mihalyi E, and Godfrey J. E. 1963; Digestion of fibrinogen by trypsin II. Characterization of the large fragment obtained. Biochimica et Biophysica Acta 67: 90.
• 23 Osbahr A. J, Gladner J. A, and Laki K. 1964; Studies on the physiological activity of the peptide released during the fibrinogen-fibrin conversion. Biochimica et Biophysica Acta 86: 535.
• 24 Wallén P. 1971 Plasmic degradation of fibrinogen. In: Verstraete M, Vermylen J, and Donati M. B. (Ed.) Fibrinogen Degradation Products. International Workshop held from July 22nd to 25th 1970, Leuven (Belgium). Scandinavian Journal of Haematology. Suppl. 13. p. 3.
• 25 Wallén P, Iwanaga S. 1968; Differences between plasmic and tryptic digests of human S-suifo-fibrinogen. Biochimica et Biophysica Acta 154: 414.
• 26 Wiśniewski K. 1972; Influence of trypsin on the INH absorption and distribution in the tissues of rats. Acta Physiologica Polonica 23: 167.
• 27 Wójciakowa Z. 1967 Application of cationitized paper for alkaloids determination in experimental acute poisonings. Medical Academy, Poznan (Manuscript), pp. 54.