Preparation of Modified Bovine Factor VIII with Enhanced Biological Activity Using Insoluble-trypsin Columns

Christine N. Vogel; Henry E. Parfitt Jr.; Henry S. Kingdon; Roger L. Lundblad

doi:10.1055/s-0038-1649069

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1973; 30(01): 229-234
DOI: 10.1055/s-0038-1649069

Original Article

Schattauer GmbH

Preparation of Modified Bovine Factor VIII with Enhanced Biological Activity Using Insoluble-trypsin Columns

Christine N. Vogel

¹Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)

,

Henry E. Parfitt Jr.

¹Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)

,

Henry S. Kingdon

¹Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)

,

Roger L. Lundblad

¹Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)

› Author Affiliations

Abstract

Summary

Crude bovine Factor VIII preparations have been modified by passage over a column of trypsin-agarose. Factor VIII prepared without an early adsorption step utilizing either barium sulfate or aluminum hydroxide gel generated significant Factor Xa activity by passage over such a column and was unsuitable for further characterization. Factor VIII prepared using adsorption with barium sulfate contained no Factor Xa activity after passage over trypsin-agarose. This trypsin-modified material showed a decreased molecular size as judged by behavior on gel filtration and an increased rate of reaction with bovine Factor IXa.

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References

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