The Effect of Molecular Iodine on Platelet Aggregation in Vitro

Summary

When rabbit platelet-rich plasma was treated with 1-2 mM molecular iodine, marked irreversible aggregation of platelets was observed. The aggregation was characterized by inhibition by 1 mM reduced glutathione, acceleration by 0.1 mM NEM, and no interference with EDTA and adenosine which are powerful inhibitors of aggregation induced by ADP. Molecular iodine at a concentration of 0.5 mM did not induce aggregation and caused more than 60% inhibition of ADP- and collagen-induced aggregation. These findings indicated that the aggregation by molecular iodine was in quite different mechanisms from those by other aggregating agents such as ADP, and suggested that platelet aggregation by molecular iodine and ADP was greatly regulated by sulfhydryl groups.

• References

• 1 Aledort L. M, Troup S. B, Weed R. I. Inhibition of sulfhydryl-dependent platelet functions by penetrating and non-penetrating analogs of parachloromercuri-benzene. Blood 1968; 31: 471
• 2 Al-Mondhiry H, Spaet T. H. Inhibition of platelet adhesion to collagen by sulfhydryl inhibitors. Proceedings of the Society for Experimental Biology and Medicine 1970; 135: 878
• 3 Ardlie N. G, Packham M. A, Mustard J. F. Adenosine diphosphate-induced platelet aggregation in suspensions of washed rabbit platelets. British Journal of Haematology 1970; 19: 7
• 4 Born G. V. R, Cross M. J. The aggregation of blood platelets. Journal of Physiology 1963; 168: 178
• 5 Born G. V. R. Mechanisms of platelet aggregation and of its inhibition by adenosine derivatives. Federation Proceedings 1967; 26: 115
• 6 Cross M. J. Effect of fibrinogen on the aggregation of platelets by adenosine diphosphate. Thrombosis et Diathesis Haemorrhagica 1964; 12: 524
• 7 Dickson R. C, Doery J. C. G, Lewis A. F. Ultraviolet light: A new stimulus for the induction of platelet aggregation. Science 1971; 172: 1140
• 8 Fantl P. Thiol groups of blood platelets in relation to clot retraction. Nature (London) 1963; 198: 95
• 9 Friedman E, Marrian D. H, Simon-Reuss I. Antimitotic action of maleimide and related substances. British Journal of Pharmacology 1949; 4: 105
• 10 Gaarder A, Jonsen J, Laland S, Hellem A, Owren P. A. Adenosine diphosphate in red cells as a factor in the adhesiveness of human blood platelets. Nature (London) 1961; 192: 531
• 11 Gaarder A, Laland S. Hypothesis for the aggregation of platelets by nu-cleotides. Nature (London) 1964; 202: 909
• 12 Harrison M. J. G, Emmons P. R, Mitchell J. R. A. The effect of sulphy-dryl and enzyme inhibitors on platelet aggregation in vitro. Thrombosis et Diathesis Haemorrhagica 1966; 16: 122
• 13 Haslam R. Role of adenosine diphosphate in the aggregation of human blood-platelets by thrombin and by fatty acids. Nature (London) 1964; 202: 765
• 14 Higashi O, KikUchi Y, Konno K. A case of thrombasthenia with a study of platelet aggregation by hydrogen peroxide. Tohoku Journal of Experimental Medicine 1972; 106: 399
• 15 Higashi O, Kikijchi Y, Konno K. Effects of metabolic inhibitors on the platelet aggregation induced by hydrogen peroxide. Tohoku Journal of Experimental Medicine 1973; 109: 245
• 16 Ichikawa Y, Shibata S, Kobayashi B. Inhibition of platelet aggregation by quinone derivatives. Seikagaku (Japan) 1972; 44: 495
• 17 Kikugawa K, Iizuka K, Higuchi Y, Hirayama H, Ichino M. Platelet aggregation inhibitors 2. Inhibition of platelet aggregation by 5’-, 2-, 6-, and 8-substitut-ed adenosines. Journal of Medicinal Chemistry 1972; 15: 387
• 18 Kikugawa K, Iizuka K. Inhibition of platelet aggregation by bisulfite-sulfite. Journal of Pharmaceutical Sciences 1972; 61: 1904
• 19 Kikugawa K, Iizuka K, Ichino M. 1973; a Platelet aggregation inhibitors 4. N⁶-Substituted adenosines. Journal of Medicinal Chemistry 16: 358
• 20 Kikugawa K, Subhiro H, Ichino M. Platelet aggregation inhibitors 6. 2-Thioadenosine derivatives. Journal of Medicinal Chemistry 1973; 16: 1381
• 21 Mustard J. F, Packham M. A. Factors influencing platelet function: adhesion, release and aggregation. Pharmacological Reviews 1970; 22: 97
• 22 Robinson Jr. C. W, Mason R. G, Wagner R. H. Effect of sulfhydryl inhibitors on platelet-agglutinability. Proceedings of the Society for Experimental Biology and Medicine 1963; 113: 857
• 23 Robinson Jr. C. W, Kress S. C, Wagner R. H, Brinkhous K. M. Platelet agglutination and deagglutination with a sulfhydryl inhibitor, methyl mercuric nitrate: relationship to platelet ATPase. Experimental and Molecular Pathology 1965; 4: 457
• 24 SkÅlhegg B. A, Hellem A. J, Ödegaard A. F. Investigation on adenosine diphosphate induced platelet adhesiveness in vitro. Part II. Studies on the mechanism. Thrombosis et Diathesis Haemorrhagica 1964; 11: 305