Platelet Metabolism during the Interiorization of Two Different Types of Particulate Matter

I. A. Cooper; P Cochrane; B. G. Firkin; K. J. Pinkard

doi:10.1055/s-0038-1649365

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1972; 27(02): 263-271
DOI: 10.1055/s-0038-1649365

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

Platelet Metabolism during the Interiorization of Two Different Types of Particulate Matter

I. A. Cooper M.B., B.S., M.R.A.C.P.

¹⁾Clinical and Research Haematologist, Cancer Institute, Melbourne, Victoria.

⁵⁾Address for reprints: Dr. I. A. Cooper, Clinical Haematologist, Cancer Institute, 278 William Street, Melbourne, Victoria 3000, Australia.

,

P Cochrane

²⁾Technical Assistant, Cancer Institute, Melbourne, Victoria.

,

B. G. Firkin B.Sc. (Med.), M.B., B.S., F.R. A.C.P.

³⁾Professor of Medicine, Monash University Medical School, Alfred Hospital, Commercial Road, Prahran, Victoria.

⁶⁾Supported by N. H. & M. R. C.

,

K. J. Pinkard M.Sc.

⁴⁾Research Assistant, Cancer Institute, Melbourne, Victoria.

› Author Affiliations

Abstract

Summary

It has been suggested that human platelets possess the ability to phagocytose particulate matter similar to the polymorphonuclear leukocyte. However some difference of opinion has arisen regarding this contention, particularly as differences have been demonstrated with regard to the observed metabolic changes occurring in platelets related to such a process.

The experiments reported in this paper were designed to observe the aerobic and anaerobic metabolism in human platelets during and following interiorization of two different particles, viz. polystyrene latex and thorotrast. The results of these experiments show a marked difference between both types of particles with regard to observable metabolic changes despite the rapid interiorization of both types of material. Some alteration occurs in both aerobic and anaerobic metabolism a considerable time after interiorization of latex, whereas no alteration could be demonstrated after interiorization of thorotrast. It is suggested that the interiorization of particulate matter is by some process other than phagocytosis and that observed metabolic changes related to latex may be due to a release reaction.

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References

References
1 Oren R, Farnham A. E, Saito K, MilofsJcy E, Karnovsky M. L. Metabolie patterns in three types of phagocytosing cells. J. Cell Biology 17: 487 1963;
2 David-Ferreira F. J. International Revue of Cytology.. Browne G. H, Daniell J. R. eds. Vol. 17, 99-148 Academic Press; New York: 1964
3 Movat H. A, Weister W. J, Glynn M. F, Mustard J. F. Platelet phagocytosis and aggregation. J. Cell Biology 27: 531 1965;
4 Kuramoto A, Steiner M, Baldini M. G. Metabolic basis of platelet phagocytosis. Biochim. biophys. Acta (Amst) 201: 471 1970;
5 Karnovsky M. F. Metabolic basis of phagocytic activity. Physiol. Rev 42: 145 1962;
6 Sbarra A. J, Karnovsky M. F. The biochemical basis of phagocytosis and metabolic changes during the ingestion of particles by polymorphonuclear leucocytes. J. biol. Chem 234: 1355 1959;
7 Loder P. B, Hirsh J, de Gruchy G. C. Effect of collagen on platelet glycolysis and nucleotide metabolism. Brit. J. Haemat 14: 563 1968;
8 Wolfe S. M, Shulman N. R. Inhibition of platelet energy production and release by P.G.E., theophylline and AMP. Biochem. biophys. Res. Commun. 41 (1) : 128 1970;
9 Jean G, Racine L, Gautier A. On the presence of neutral fats in normal and pathological human thrombocytes. Thrombos. Diathes. haemorrh. (Stuttg) 09: 1 1963;
10 Firkin B. G, O’Neill B. J, Dunstan B, Oldfied R. The effect of incubation and storage on human platelet structure as studied by electron microscopy. Blood 25: 345 1965;