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Thromb Haemost 1966; 15(03/04): 511-518
DOI: 10.1055/s-0038-1649453
Originalarbeiten — Original Articles — Travaux Originaux
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Preparation of Human Plasminogen Suitable as Substrate for Quantitative Determination of Plasminogen Activators

W Berg
1   Medical Department II, Sahlgrenska Hospital, University of Göteborg, Sweden
,
K Korsan-Bengtsen
1   Medical Department II, Sahlgrenska Hospital, University of Göteborg, Sweden
,
J Ygge
1   Medical Department II, Sahlgrenska Hospital, University of Göteborg, Sweden
› Author Affiliations
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Publication History

Publication Date:
24 July 2018 (online)

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Summary

A simple method for preparation of plasminogen with low spontaneous activity and soluble at a neutral pH and at physiological ionic strength is described. Euglobulin made from fresh, oxalated, BaSO4-adsorbed, human plasma was first purified by means of gel filtration on Sephadex G-200. After gel filtration, further purification and concentration was done on DEAE-sephadex A-50. The activity was 100-130 casein units per mg tyrosine.

 

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