Prothrombin Purification by Chromatography^[*]

Summary

Bovine prothrombin with specific activity above 40,000 u/mg of tyrosine may be obtained by chromatography on Amberlite AG3-X4. The product does not yield autoprothrombin C in appreciable amounts. A fraction separated by this procedure behaves as a procoagulant when replaced into the purified prothrombin.

^* This work was supported by Research Grant HE-06-439 from the National Institutes of Health, U.S. Public Health Service, and by the Rockefeller Foundation.

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