Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1968; 19(03/04): 316-320DOI: 10.1055/s-0038-1651210 Originalarbeiten – Original Articles – Travaux Originaux Schattauer GmbH Prothrombin Purification by Chromatography[*] R. H Landaburu 1 Institute of Pharmacology, National University of Córdoba, Córdoba, Argentina , Emilia Albado 1 Institute of Pharmacology, National University of Córdoba, Córdoba, Argentina , R Santillán 1 Institute of Pharmacology, National University of Córdoba, Córdoba, Argentina› Author AffiliationsRecommend Article Abstract Buy Article Summary Bovine prothrombin with specific activity above 40,000 u/mg of tyrosine may be obtained by chromatography on Amberlite AG3-X4. The product does not yield autoprothrombin C in appreciable amounts. A fraction separated by this procedure behaves as a procoagulant when replaced into the purified prothrombin. Full Text References References 1 Seegers W.H. The purification of prothrombin. Rec. Chem. Progr. 1952; 13: 143 2 Miller K.D. Rivanol, resin and the isolation of thrombins. Nature (Lond.) 1959; 184: 450 3 Seegers W.H, Landaburu R.H. Purification of prothrombin and thrombin by chromatography on cellulose. Canad. J. Biochem. 1960; 38: 1405 4 Seegers W.H. Prothrombin. Harvard University Press: Cambridge Mass.; 1962: 22-26 5 Seegers W.H, Marciniak E, Cole E.R. Autocatalysis in prothrombin activation. Amer. J. Physiol. 1962; 203: 397 6 Marciniak E, Seegers W.H. Prethrombin as a new sub-unit of prothrombin. Nature (Lond.) 1966; 209: 621 7 Seegers W.H, Cole E.R, Aoki N, Harmison G.R. Separation of autoprothrombin III from bovine prothrombin preparations. Canad. J. Biochem. 1964; 42: 229 8 Milstone J.H. TAMe esterase activity of blood thrombokinase after repeated electrophoretic fractionation. Proc. Soc. exp. Biol. (N. Y.) 1960; 103: 361 9 Bergsagel D.E, Hougie C. Intermediate stages in the formation of blood thromboplastin. Brit. J. Haemat. 1956; 2: 113 10 Zucker-Franklin D. J, avid J, Spaet T.H. Characterization of blood coagulation product I. Fed. Proc. 1961; 20: 53 11 Landaburu R.H, Gurtino J. A standard preparation of bovine fibrinogen. Thrombos. Diathes. haemorrh. (Stuttg.) 1954; 12: 64 12 Alkjaersig N, Abe T, Seegers W.H. Purification and quantitative determination of platelet factor 3. Amer. J. Physiol. 1955; 181: 304 13 Seegers W.H, Cole E.R, Harmison G.R, Marciniak E. Purification and some properties of autoprothrombin C. Canad. J. Biochem. 1963; 41: 1047 14 Seegers W.H, Smith H.P. Factors which influence the activity of purified thrombin. Amer. J. Physiol. 1942; 137: 348 15 Gole E.R, Marciniak E, Seegers W.H. Procedures for the quantitative determination of autoprothrombin C. Thrombos. Diathes. haemorrh. (Stuttg.) 1962; 8: 434 16 Landaburu R.H, Abdala O, Fulginiti S, Gurtino J. Studies on autoprothrombin C thrombin relationship. Thrombos. Diathes. haemorrh. (Stuttg.) 1965; 14: 176 17 Seegers W.H, Marciniak E. Autoprothrombin C in irregular blood clotting. Thrombos. Diathes. haemorrh. (Stuttg.) 1962; 8: 1 18 Tibbs J. Identification of the free carboxo-groups in peptides and proteins. Nature (Lond.) 1951; 168: 910 19 Lechner K, Deutsch E. Activation of factor X. Thrombos. Diathes. haemorrh. (Stuttg.) 1965; 13: 314
