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Thromb Haemost 1975; 34(01): 216-222
DOI: 10.1055/s-0038-1651369
Original Article
Schattauer GmbH

Soluble Fibrin Monomer Complexes Demonstrated by Agarose Gel Filtration and by Adsorption on Insolubilized Fibrinogen

A Comparative Study
R von Hugo
1   I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany
2   Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany
,
R Hafter
1   I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany
2   Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany
,
A Stemberger
1   I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany
2   Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany
,
H Graeff
1   I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany
2   Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany
› Author Affiliations
Further Information

Publication History

Received 05 December 1974

Accepted 08 March 1975

Publication Date:
02 July 2018 (online)

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Summary

Incubation of fibrinogen with small amounts of thrombin resulted in the occurrence of soluble fibrin monomer complexes. These complexes consisted predominantly of a derivative with a higher molecular weight than that of fibrinogen. It was characterized by its relative electrophoretic mobility in 5% PAA gel (0.28 × 10-5 cm2/V × sec) and its elution position prior to the fibrinogen peak following gel filtration. Using adsorption chromatography on insolubilized fibrinogen the derivative dissociated at a ratio of almost 1 : 1 into one part which was adsorbed and into fibrinogen which was not adsorbed. The part which was adsorbed seemed to be the thrombin mediated fibrin monomer. This study confirms the concept that dissociable dimeric fibrinogen-fibrin monomer complexes occur after limited action of thrombin on fibrinogen.

 

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