Soluble Fibrin Monomer Complexes Demonstrated by Agarose Gel Filtration and by Adsorption on Insolubilized Fibrinogen

R von Hugo; R Hafter; A Stemberger; H Graeff

doi:10.1055/s-0038-1651369

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1975; 34(01): 216-222
DOI: 10.1055/s-0038-1651369

Original Article

Schattauer GmbH

Soluble Fibrin Monomer Complexes Demonstrated by Agarose Gel Filtration and by Adsorption on Insolubilized Fibrinogen

A Comparative Study

R von Hugo

¹I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany

²Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany

,

R Hafter

¹I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany

²Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany

,

A Stemberger

¹I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany

²Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany

,

H Graeff

¹I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany

²Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany

› Author Affiliations

Abstract

Summary

Incubation of fibrinogen with small amounts of thrombin resulted in the occurrence of soluble fibrin monomer complexes. These complexes consisted predominantly of a derivative with a higher molecular weight than that of fibrinogen. It was characterized by its relative electrophoretic mobility in 5% PAA gel (0.28 × 10^-5 cm²/V × sec) and its elution position prior to the fibrinogen peak following gel filtration. Using adsorption chromatography on insolubilized fibrinogen the derivative dissociated at a ratio of almost 1 : 1 into one part which was adsorbed and into fibrinogen which was not adsorbed. The part which was adsorbed seemed to be the thrombin mediated fibrin monomer. This study confirms the concept that dissociable dimeric fibrinogen-fibrin monomer complexes occur after limited action of thrombin on fibrinogen.

Full Text

References

References
19 Beller F. K, and Graeff H. 1970. Equipment and general requirements of the coagulation laboratory. In: Bang N, Beller F. K, Deutsch E, and Mammen E. (eds.) Thrombosis and Bleeding Disorders. Academic Press; New York: Thieme, Stuttgart 57.
20 Fletcher A. P, Alkjaersig N, and O’Brien J. 1970. Blood hypercoagulability and thrombosis. In: Abstract volume, XIII. International Congress of Hematology. Munich: 2-8 August 1970: 244.
21 Godal H, and Abildgaard U. 1966; Gelation of soluble fibrin in plasma by ethanol. Scandinavian Journal of Haematology 3: 342.
22 Graeff H, and von Hugo R. 1972; Identification of fibrinogen derivatives in plasma samples. Thrombosis et Diathesis Haemorrhagica 27: 610.
23 Graeff H, von Hugo R, and Hafter R. 1973; In vivo formation of soluble fibrin monomer complexes in human plasma. Thrombosis Research 3: 465.
24 Graeff H, and von Hugo R. 1974; Fibrinogen derivatives in a case of abruptio placentae. American Journal of Obstetrics and Gynecology 120: 335.
25 Graeff H, Wiedemann A, von Hugo R, and Hafter R. 1975 Amount and distribution pattern of soluble fibrin monomer complexes during the early puerperium. American Journal of Obstetrics and Gynecology, in press.
26 Heene D. L, and Matthias F. R. 1973; Adsorption of fibrinogen derivatives on insolubilized fibrinogen and fibrinmonomer. Thrombosis Research 2: 137.
27 Yon Hugo R, and Graeff H. 1973; Formation of soluble fibrin monomer complexes in human plasma. Thrombosis Research 3: 183.
28 Kierulf P. 1973; Studies on soluble fibrin in plasma. IV. Isolation and characterization of the clottable proteins obtained from thrombin incubated plasma upon gelation with ethanol. Thrombosis Research 3: 613.
29 Shainoff J. R, and Page J. H. 1962; Significance of cryoprofibrin in fibrinogen-fibrin conversion. Journal of Experimental Medicine 3: 183.
30 Stemberger A, and Hörmann H. 1974 Adsorption chromatography of plasma fibrin on insolubilized fibrinogen. (submitted for publication).
31 Vermylen J, Donati M. B, and Yerstraete M. 1971. The identification of fibrinogen derivatives in plasma and serum by agarose gel filtration. In: Yerstraete M, Vermylen J, and Donati M. B. (eds.) Fibrinogen Degradation Products. Scandinavian Journal of Haematology, Supplementum, vol. 13 219.