Studies in Experimental Animals on Disseminated Intravascular Coagulation (DIC)

F Markwardt; G Nowak; W Meerbach; K.-D Rüdiger

doi:10.1055/s-0038-1651410

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1975; 34(02): 513-521
DOI: 10.1055/s-0038-1651410

Original Article

Schattauer GmbH

Studies in Experimental Animals on Disseminated Intravascular Coagulation (DIC)

Authors

F Markwardt

¹Institute of Pharmacology and Toxicology and the Institute of General Pathology and Pathological Anatomy, Medical Academy Erfurt, Erfurt (GDR)
G Nowak

¹Institute of Pharmacology and Toxicology and the Institute of General Pathology and Pathological Anatomy, Medical Academy Erfurt, Erfurt (GDR)
W Meerbach

¹Institute of Pharmacology and Toxicology and the Institute of General Pathology and Pathological Anatomy, Medical Academy Erfurt, Erfurt (GDR)
K.-D Rüdiger

¹Institute of Pharmacology and Toxicology and the Institute of General Pathology and Pathological Anatomy, Medical Academy Erfurt, Erfurt (GDR)

Abstract

Summary

Changes in the clotting system, as well as morphological and functional alterations corresponding to that of the pathologic phenomenon of disseminated intravascular coagulation (DIC) or consumption coagulopathy, were produced by thrombin infusion (550 NIH U × kg⁻¹ × h⁻¹) in rats and simultaneous inhibition of fibrinolysis by PAMBA (100 mg/kg).

Changes in the fibrinogen level and platelet count as well as the appearance of fibrin monomers and the formation of microthrombi in several organs were evaluated. Simultaneously, the function of the respiratory system was investigated by continuous measurement of oxygen consumption as well as elasticity and water content of the lung. From the time course of the alterations in the several parameters, conclusions can be drawn for the pathogenesis and the possible therapeutic influence on DIC.

Full Text

References

References
1 Beller F. K. 1971. Experimental animal models for the production of disseminated intravascular coagulation. In: Bang N. U, Beller F. K, Deutsch E, and Mammen E. F. (eds) Thrombosis and Bleeding Disorders. Academic Press; New York, London: 514.
2 Beller F. K, Mitchell P. S, and Gorstein F. 1967; Fibrin deposition in the rabbit kidney produced by protease inhibitors. Thrombosis et Diathesis Haemorrhagica 17: 427.
3 Beller F. K, and Theiss W. 1973; Fibrin derivatives, plasma hemoglobin and glomerular fibrin deposition in experimental intravascular coagulation. Thrombosis et Diathesis Haemorrhagica 24: 363.
4 Betke K, and Savelsberg W. 1950; Stufenphotometrische Haemoglobinbestimmung mittels Cyanhaemoglobin. Biochemische Zeitschrift 320: 431.
5 Brain M. C, and Hourihane D. O. 1967; Microangiopathic haemolytic anaemia: The occurrence of haemolysis in experimentally produced vascular disease. British Journal of Haematology 13: 135.
6 Busch Ch, Lundquist H, and Saldeen T. 1971; Quantitative determination of intravascular coagulation in vivo in various organs in the dog. Thrombosis et Diathesis Haemorrhagica 26: 315.
7 Busch Ch, Rammer L, and Saldeen T. 1973; Quantitation of fibrin deposition and elimination in organs of rats injected with labelled fibrinogen and albumin. Thrombosis et Diathesis Haemorrhagica 29: 94.
8 Busch Ch, and Saldeen T. 1973; Amount of fibrin in different organs after intravenous, intraportal and intraaortal injection of thrombin in the rat. Thrombosis et Diathesis Haemorrhagica 29: 87.
9 Deutsch E, and Lechner K. 1971; Die Klinik der manifesten generalisierten intravaskulären Gerinnung. Thrombosis et Diathesis Haemorrhagica Suppl.50: 57.
10 Godal H. C, and Abildgaard U. 1966; Gelation of soluble fibrin in plasma by ethanol. Scandinavian Journal of Haematology 3: 432.
11 Graeff H, and Beller F. K. 1968; Fibrinolytic enzyme system of the kidney related to renal function after infusion of endotoxin in rabbits. Laboratory Investigation 19: 169.
12 Hardaway R. M. 1966. Syndromes of Disseminated Intravascular Coagulation. Thomas; Springfield, Ill:
13 Landmann H. 1973; Studies on the mechanism of action of synthetic antifibrinolytics. A comparison with the action of derivatives of benzamidine on the fibrinolytic process. Thrombosis et Diathesis Haemorrhagica 29: 253.
14 Lasch H. G, Heene D. L, Huth K, and Sandritter W. 1967; Pathophysiology, clinical manifestation and therapy of consumption-coagulopathy (Verbrauchskoagulopathie). The American Journal of Cardiology 20: 381.
15 Maegaretten W, Csavossy I, and McKay D. G. 1967; An electron microscopic study of thrombin-induced disseminated intravascular coagulation. Blood 29: 169.
16 Margaretten W, Zunker H. O, and McKay D. G. 1964; Production of the generalized Shwartzman reaction in pregnant rats by intravenous infusion of thrombin. Laboratory Investigation 13: 552.
17 Markwardt F, Klöcking H.-P, and Nowak G. 1970; Antithrombin-und Anti-plasminwirkung von 4-Amidinophenylbrenztraubensäure (APPA) in vivo. Thrombosis et Diathesis Haemorrhagica 24: 240.
18 Markwardt F, Landmann H, and Klöcking H.-P. 1972. Fibrinolytika und Anti-fibrinolytika. Fischer; Jena:
19 McKay D. G. 1965. Disseminated Intravascular Coagulation. An Intermediary Mechanism of Disease. P. B. Hoeber; New York:
20 Müller-Berghaus G. 1966; Pathophysiology of disseminated intravascular coagulation. Thrombosis et Diathesis Haemorrhagica Suppl.36: 45.
21 Nowak G, and Koth W. 1975; Kontinuierliche Messung des Sauerst off Verbrauchs bei kleinen Versuchstieren. Acta Biologica et Medica Germanica 34: 1037.
22 Regoeczi E, Kubenberg M. L, and Brain M. C. 1967; Intravascular haemolysis and disseminated intravascular coagulation. Lancet 1: 601.
23 Rüfer K, and Stolz C. 1969; Inaktivierung von alveolären Oberflächenfilmen durch Erniedrigung der Oberflächenspannung der Hypophase. European Journal of Physiology 307: 89.
24 Saldeen T. 1969; Quantitative determination of intravascular coagulation in the lungs of experimental animals. Scandinavian Journal of Haematology 6: 205.
25 Schoendorf T, Rosenberg M, and Beller F. K. 1971; Endotoxin-induced disseminated intravascular coagulation in non-pregnant rats: A new experimental model. American Journal of Pathology 65: 51.
26 Seegers W. H, McCoy L, Marciniak E, and Murano G. 1969; Theory of blood coagulation: Applications in disseminated intravascular coagulation. Thrombosis et Diathesis Haemorrhagica Suppl.36: 239.
27 Walsmann P. 1973; Über die Reinigung von Thrombinpräparaten. Die Pharmazie 23: 401.