Thromb Haemost 1975; 34(03): 795-805
DOI: 10.1055/s-0038-1651472
Original Article
Schattauer GmbH

Collagen-Induced Platelet Aggregation: The Role of Adenine Nucleotides and the Release Reaction

Herman Kattlove
1   Department of Medicine, Harbor General Hospital, Torrance, California
2   UCLA School of Medicine, Los Angeles, California, USA
,
M. Hugo Gomez
1   Department of Medicine, Harbor General Hospital, Torrance, California
2   UCLA School of Medicine, Los Angeles, California, USA
› Author Affiliations
Further Information

Publication History

Received 04 May 1975

Accepted 29 May 1975

Publication Date:
02 July 2018 (online)

Summary

Collagen-induced platelet aggregation is associated with the release of ADP and the catabolism of radioactive ATP. These studies demonstrate that the amount of human platelet ADP released and ATP catabolized increase with time of stirring of the collagen-platelet rich plasma (PRP) mixture and with increasing amounts of collagen. These changes in adenine nucleotides occurred simultaneously with the aggregation. No early changes preceding aggregation were noted. Stirring the collagen-PRP mixture maximized ADP release and ATP catabolism; some ADP release and ATP catabolism occurred with minimal agitation. Incubation of PRP with metabolic poisons (2-deoxyglucose with either KCN or oligomycin), which lowered platelet ATP content, also reduced collagen-induced release of ADP and aggregation. However, platelet adhesion to collagen was unaffected by metabolic poisons. These data suggest that collagen directly stimulates ADP release. The demonstration of release in EDTA–PRP further suggests that platelet aggregation is not required for collagen-induced ADP release.

 
  • References

  • 1 Ball G, Fulwood M, Ireland D. M, and Yates P. 1969; Effect of some inhibitors of platelet aggregation on platelet nucleotides. Biochemical Journal 114: 669.
  • 2 Bounameaux Y. 1959; L’accolement des plaquettes fibres sous endotheliales. Comptes Rendus des Seances de la Société de Ses Filiales, Paris 153: 865.
  • 3 Gaarder A, Jonsen J, Lalano S, Hellem A, and Owren P. A. 1961; Adenosine diphosphate in red cells as a factor in the adhesiveness of human blood platelets. Nature 192: 531.
  • 4 Holmsen H, Day H. J, and Storm E. 1969; a Adenine nucleotide metabolism of blood platelets. VI. Subcellular localization of nucleotide pools with different functions in the platelet release reaction. Biochimica et Biophysica Acta 186: 254.
  • 5 Holmsen H, Day H. J, and Stormorken H. 1969 b The blood platelet release reaction. Scandinavian Journal of Haematology. Suppl. 8.
  • 6 Holmsen H, Setkowsky C. A, and Day H. J. 1974; Effects of antimycin and 2-deoxyglucose on adenine nucleotides in human platelets. Biochemical Journal 144: 385.
  • 7 Holmsen H, Storm E, and Day H. J. 1972; Determination of ATP and ADP in blood platelets: a modification of the firefly luciferase assay for plasma. Analytical Biochemistry 46: 489.
  • 8 Holmsen H, and Weiss H. J. 1970; Hereditary defect in the platelet release reaction caused by a deficiency in the storage pool of platelet adenine nucleotides. British Journal of Haematology 19: 643.
  • 9 Hovig T. 1963; Release of a platelet aggregating substance (adenosine diphosphate) from rabbit blood platelets induced by saline “extract” of tendons. Thrombosis et Diathesis Haemorrhagica 9: 264.
  • 10 Hugues J. 1960; Accolement des plaquettes au collagene. Comptes Rendus des Seances de la Société de Ses Filiales, Paris 154: 866.
  • 11 Jamieson G. A, Urban C. L, and Barber A. J. 1971; Enzymatic basis for platelet: collagen adhesion as the primary step in hemostasis. Nature New Biology 234: 5.
  • 12 Kattlove H. 1974; Platelet ATP in ADP-induced aggregation. American Journal of Physiology 226: 325.
  • 13 Macmillan D. C. 1966; Secondary clumping effect in human citrated platelet-rich plasma produced by adenosine diphosphate and adrenalin. Nature 211: 140.
  • 14 Massini P, and Luscher E. F. 1972; On the mechanism by which cell contact induces the release reaction of blood platelets ; the effect of cationic polymers. Thrombosis et Diathesis Haemorrhagica 27: 121.
  • 15 Massini P, and Lüscher E. F. 1971; The induction of the release reaction in human platelets by close cell contact. Thrombosis et Diathesis Haemorrhagica 25: 13.
  • 16 Mills D. C. B, Robb I. A, and Roberts G. C. K. 1968; The release of nucleotides, 5-hydroxytryptamine and enzymes from human blood platelets during aggregation. Journal of Physiology 195: 715.
  • 17 Murer E. H. 1968; Release reaction and energy metabolism in blood platelets with special reference to the burst in oxygen uptake. Biochimica et Biophysica Acta 162: 320.
  • 18 Nunn B. 1972; The role of adenosine diphosphate (ADP) in collagen-induced aggregation. British Journal of Pharmacology 46: 579.
  • 19 Packham M. A, Guccione M. A, Chang P, and Mustard J. F. 1973; Platelet aggregation and release: effects of low concentrations of thrombin or collagen. American Journal of Physiology 225: 38.
  • 20 Spaet T. H, and Lejnieks I. 1969; A technique for estimation of platelet-collagen adhesion. Proceedings of the Society for Experimental Biology and Medicine 132: 1038.
  • 21 Spaet T. PL, Lejnieks I, Gaynor E, and Goldstein M. L. 1969; Defective platelets in essential thrombocythemia. Archives of Internal Medicine 124: 135.
  • 22 Spaet T. H, and Zucker M. B. 1964; Mechanism of platelet plug formation and role of adenosine diphosphate. American Journal of Physiology 206: 1267.
  • 23 Zucker B, and Borrelli J. 1962; Platelet clumping produced by connective tissue suspensions and by collagen. Proceedings of the Society for Experimental Biology and Medicine 109: 779.