Studies on the Neutralization of Rabbit Antibodies to Human Factor VIII

P. B. A Kernoff; A. C Kaelin

doi:10.1055/s-0038-1651887

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1977; 38(03): 0707-0716
DOI: 10.1055/s-0038-1651887

Original Article

Schattauer GmbH

Studies on the Neutralization of Rabbit Antibodies to Human Factor VIII

P. B. A Kernoff^*

¹Oxford Haemophilia Centre, Churchill Hospital, Oxford, OX3 7LJ, England

,

A. C Kaelin^**

¹Oxford Haemophilia Centre, Churchill Hospital, Oxford, OX3 7LJ, England

› Author Affiliations

Abstract

Summary

The capacity of normal and haemophilic cryoprecipitates to neutralize the anticoagulant effect of rabbit antibodies to human factor VIII (anti-VIII) was assessed using a quantitative estimation of antibody. About 4 times as much anti-VIII could be neutralized by normal factor VIII as was required to neutralize clotting activity. This suggests that there are probably several antigenic sites intimately associated with factor VIII clotting activity, quite apart from any antigenic sites which may be detected using antibodies directed against other components of the factor VIII complex. The neutralizing capacity of factor VIII was only slightly greater for the rabbit antibodies employed in this study than has been previously reported for antibodies of human origin, thus indicating only minor differences in specificities. Additional evidence in support of this concept was the finding that cryoprecipitates prepared from haemophilic plasmas previously recognized as either lacking or possessing the capacity to neutralize antibodies of human origin neutralized least or most quantities of rabbit antibodies, respectively.

Full Text

References

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