Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1971; 26(01): 077-082DOI: 10.1055/s-0038-1653657 Originalarbeiten – Original Articles – Travaux Originaux Schattauer GmbH Fibrinolysis Induced by Factor XII Contained in Prothrombin Preparations Authors Author Affiliations R. H Landaburu 1 Institute of Pharmacology, National University of Cordoba, Cordoba, Argentina C Pernici 1 Institute of Pharmacology, National University of Cordoba, Cordoba, Argentina D. E Castellanos 1 Institute of Pharmacology, National University of Cordoba, Cordoba, Argentina Recommend Article Abstract Buy Article(opens in new window) Summary Prothrombin products prepared by Seegers procedure induce fibrinolysis when injected intravenously. This property is due to a contaminant denominated Prothrombin Activating Derivative-B, identified as factor XII. The fibrinolytic fraction accompanies prothrombin throughout the purification procedure and may be separated by chromatography on DEAE cellulose. Full Text References References 1 Celander D. R, Guest M. M. Euglobulin lysis time. In: Blood coagulation, hemorrhage and thrombosis. 249-250 2nd. ed. Edited by Tocantins L. M, Kazal L. A. Grune & Stratton, Inc; New York: 1964 2 Guest M. M. Estimation of fibrinolytic activity of plasma or serum: Fibrin plate method, unheated and heated (Methods of Astrup and Mullertz and Lassen). In: Blood coagulation, hemorrhage and thrombosis. 246-248 2nd. ed. Edited by Tocantins L. M, Kazal L. A. Grune & Stratton, Inc; New York: 1964 3 Hunter D. T, Allensworth J. Quantitative assay of the Hageman factor. Amer. J. clin. Path 47: 448 1967; 4 Iatridis S. G, Ferguson J. H. Effect of surface and Hageman factor on the endogenous or spontaneous activation of the fibrinolytic system. Thrombos. Diathes. haemorrh. (Stuttg.) 06: 411 1961; 5 Kowalski E, Budzyński A. Z, Kopeć M, Latallo Z. S, Lipiński B, Wegrzynowicz Z. Circulating fibrinogen degradation products (FDP) in dog blood after intravenous thrombin in fusion. Thrombos. Diathes. haemorrh. (Stuttg.) 13: 12 1965; 6 Landaburu B. H, Albado E. Factor X precursor in purified bovine prothrombin preparations. Coagulations 02: 269 1969; 7 Landaburu B. H, Albado E. Activation of factor X in bovine prothrombin preparations. Thrombos. Diathes. haemorrh. (Stuttg.) 24: 338 1970; 8 Landaburu B. H, Abdala O, Morrone M. J. Proteolytic enzymes derived from prothrombin. Canad. J. Biochem 43: 417 1965; 9 Landaburu B. H, Gurtino J. A standard preparation of bovine fibrinogen. Thrombos. Diathes. haemorrh. (Stuttg.) 12: 64 1964; 10 Landaburu B. H, Giavedoni E, Albado E. Factor XII in boyine prothrombin preparations: A purification procedure. Coagulation. (In press.) 11 Landaburu B. H, Giavedoni E, Santillân B. Thrombin and acetylated thrombin in the activation of fibrinolysis. Canad. J. Physiol. Pharmacol 46: 809 1968; 12 Landaburu B. H, Lima F, Albado E. Activation characteristics of bovine prothrombin preparations. Brit. J. Haemat 17: 83 1969; 13 Landaburu B. H, Seegers W. H. The acétylation of thrombin. Canad. J. Biochem 37: 1361 1959; 14 Moser K. M, Frey MBelle. Comparison of caseinolytic and fibrinolytic assays for plasmin (fibrinolysin) in fibrinolytic agents. Thrombos. Diathes. haemorrh. (Stuttg.) 15: 252 1966; 15 Ogston D, Ogston C. M, Batnoff O. D, Forbes C. D. Studies on a complex mechanism for the activation of plasminogen by kaolin and by chloroform : the participation of Hageman factor and additional cofactors. J. clin. Invest 48: 1786 1969; 16 Batnoff O. D, Menzie C. New method for determination of fibrinogen in small samples of plasma. J. Lab. clin. Med 37: 316 1951; 17 Seegers W. H. The purification of prothrombin. Record. Chem. Progr. (Kreske-Hocker Sci. Lib.) 13: 143 1952; 18 Seegers W. H, Landaburu B. H, Johnson J. F. Thrombin-E as a fibrinolytic enzyme. Science 131: 726 1960; 19 Seegers W. H, Levine W. G, Shepard B. S. Further studies on the purification of thrombin. Canad. J. Biochem 36: 603 1958; 20 Seegers W. H, Smith H. P. Factors which influence the activity of purified thrombin. Amer. J. Physiol 137: 348 1942; 21 Ware A. G, Seegers W. H. Two-stage procedure for the quantitative determination of prothrombin concentration. Amer. J. clin. Pathol 19: 471 1949;
