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DOI: 10.1055/s-0038-1653660
A Simplified Method for Preparation of Fibrinogen
Publication History
Publication Date:
28 June 2018 (online)
Summary
A method for preparation of fibrinogen from human plasma is presented. The method, a modification of the glycine precipitation procedure of Kazal (6), is simple and rapid, requiring 8 hours or less. The fibrinogen solution is stable on storage in the refrigerator for at least 7 months, represents an average yield of 30-40% of that in the starting material, and is around 95% clottable. Clottability is good even on storage at 37° C for several days; it is generally around 80% clottable after this storage period. The solution is free of plasminogen and plasmin but contains trace amounts of FSF (fibrin stabilizing factor) and factor VIII (factor VIII activity tends to disappear with storage). Ultracentrifugal analysis shows a main peak comprising approximately 95% of the total area (apparent sedimentation coefficient 5.8 × 10-13) and a smaller peak comprising approximately 5% or less of the total area (apparent sedimentation coefficient 3-3.5 × 10−13).
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References
- 1 Astrup T, Mullertz S. The fibrin plate method for estimating fibrinolytic activity. Arch Biochem 40: 346 1952;
- 2 Blombach B, Blombach M. Purification of human and bovine fibrinogen. Arkiv. for Kemi 10 (29) 415 1956;
- 3 Brown M. E, Rothstein F. Fibrinogen from human plasma: preparation by precipitation with heavy-metal coordination complex. Science 155: 1017 1967;
- 4 Ellis B. G, Stransky A. A quick and accurate method for the determination of fibrinogen in plasma. J. Lab. clin. Med 58: 477 1961;
- 5 Ferry J. D, Morrison P. R. Preparation and properties of serum and plasma protein. J. Amer. chem. Soc 69: 388 1947;
- 6 Kazal L. A, Amsel S, Miller O. P, Tocantins L. M. The preparation and some properties of fibrinogen precipitated from human plasma by glycine. Proc. Soc. exp. Biol. (IST. Y) 113: 989 1963;
- 7 Lahi K. The polymerization of proteins: the action of thrombin on fibrinogen. Arch Bio« chem 32: 317 1951;
- 8 Lassen M. Heat dénaturation of plasminogen in the fibrin plate method. Acta physiol, scand 27: 371 1952;
- 9 Lorand L, Jacobsen A. Studies on the polymerization of fibrin. The role of the globulin: fibrin stabilizing factor. J. biol. Chem 230: 421 1958;
- 10 Mosesson M. W. The preparation of human fibrinogen free of plasminogen. Biochim. biophys. Acta (Amst) 57: 204 1962;
- 11 Mullertz S, Lassen M. An activator system in blood indispensable for formation of plasmin by streptokinase. Proc. Soc. exp. biol. (N. Y) 82: 264 1953;
- 12 Straughn W, Wagner R. H. A simple method for preparing fibrinogen. Thrombos. Diathes. haemorrh. (Stuttg) 16: 198 1966;
- 13 Surgenor D. M, Noerther J. F. Preparation and properties of serum and plasma proteins. XXXIII. Specific interactions of prothrombin and other proteins with barium sulfate. J. Amer. chem. Soc 74: 3448 1952;