Study of Thrombin-Coagulase

J. P Soulier; Odette Prou-Wartelle; Liliane Hallé; with the technical assistance of

doi:10.1055/s-0038-1654156

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1967; 17(03/04): 321-334
DOI: 10.1055/s-0038-1654156

Originalarbeiten - Original Articles - Travaux Originaux

Schattauer GmbH

Study of Thrombin-Coagulase

Authors

J. P Soulier

¹Centre National de Transfusion Sanguine, Paris
Odette Prou-Wartelle

¹Centre National de Transfusion Sanguine, Paris

with the technical assistance of

Liliane Hallé

¹Centre National de Transfusion Sanguine, Paris

Abstract

Summary

The preparation of thrombin-coagulase is described. The properties of thrombin-coagulase are compared with those of biothrombin: kinetics, thermostability, adsorption, chromatography, esterase activity, clotting activity, action on platelets and on factors V and VIII, susceptibility to inhibitors.

Biothrombin and thrombin-coagulase are closely related but distinct. Both apparently derive from prothrombin. Prothrombin and coagulase combine to form a complex: thrombin-coagulase wherein both factors are necessary for its activity. Possible explanations for the difference between thrombin-coagulase and biothrombin are proposed.

Full Text

References

References
1 Aronson D. L, Ménaché D. Chromatographic analysis of the activation of human prothrombin with human thrombokinase. Biochemistry 5: 2635 1966;
2 Biggs R, Macfarlane R. G. Human blood coagulation and its disorders. Blackwell Scientific Publications; Oxford: 3rd Edition. 1962
3 Drummond M. C, Tager M. Enzymatic activities associated with clotting of fibrinogen by staphylocoagulase and coagulase reacting factor and their inhibition by diisopropylfluoro-phosphate. J. Bacteriol 83: 975 1962;
4 Duthie E. S, Lorenz L. L. Staphylococcal coagulase: the nature of plasma activator in the clotting process. Nature (Lond.) 165: 729 1950;
5 Gerheim E. B, Ferguson J. H. Staphylocoagulation in plasmas of various animal species. Proc. Soc. exp. Biol. (N. Y.) 71: 258 1949;
6 Haughton G, Duthie E. S. The activation of staphylococcal free coagulase by plasma constituents and the hydrolysis of N-toluene-p-sulfonyl-L-arginine methyl ester (TAMe) by activated coagulase. Biochem. J 71: 348 1959;
7 Josso F, Prou-Wartelle O, Alagille D, Soulier J. P. Le déficit congénital en facteur stabilisant de la fibrine (facteur XIII). Etude de deux cas. Nouv. Rev. franç. Hémat 4: 267 1964;
8 Koller F, Loeliger E. A, Duckert F. Le facteur II. Sa fonction dans la coagulation. Son importance clinique. Rev. Hémat 7: 156 1952;
9 Lavergne J. M. Utilisation des méthodes immunologiques pour l’étude des protéines plasmatiques de la coagulation. Application à l’étude de la prothrombine. Thèse, Paris 1966.
10 Loeb L. The influence of certain bacteria on the coagulation of the blood. J. med. Res 10: 407 1903;
11 Markwardt F. Blutgerinnungshemmende Wirkstoffe aus blutsaugenden Tieren. VEB Gustav Fischer; Jena: 1963
12 Niewiarowski S, Blatrix Ch. J, Soulier P. Adsorption de la thrombine par les particules de silice. Path. Biol 7: 2439 1959;
13 Owren P. A. A quantitative one-stage method for the assay of prothrombin. Scand. J. clin. Lab. Invest 1: 81 1949;
14 Quick A. J. The determination of prothrombin by the method of Quick. Blood Clotting and Allied Problems. (Josiah Macy Jr. Fondation, edit.) New York 1: 170 1948;
15 Rubinstein H. M. Studies on the mechanism of action of staphylocoagulase. Brit. J. Haemat 4: 326 1958;
16 Smith W, Hale J. H. Nature and mode of action of staphylococcus coagulase. Brit. J. exp. Path 25: 101 1944;
17 Soulier J. P, Prou-Wartelle O. Staphylocoagulase and prothrombin. Transactions of the Conference held under the auspices of the International committee on Blood clotting factors. St. Moritz, Switzerland, September 1965. “Diffuse Intravascular Clotting.” Schattauer. Stuttgart: 1966: 255.
18 Soulier J. P, Prou-Wartelle O. Etude comparative des taux de cofacteur de la staphylocoagulase (C. R. F.) et des taux de facteur II (prothrombine) dans diverses conditions. Nouv. Rev. franç. Hémat 6: 623 1966;
19 Soulier J. P, Lewi S, Pauty A. M, Prou-Wartelle O. Préparation, concentration et étalonnage de la staphylocoagulase. A paraître Rev. franç. Etud. clin. biol.
20 Soulier J. P, Larrieu M. J. Nouvelle méthode de diagnostic de l’hémophilie. Dosage des facteurs anti-hémophiliques A et B. Sang 24: 205 1953;
21 Soulier J. P, Weilland C. Purification de la prothrombine par la bentonite. I. Préparation de la prothrombine purifiée. IL Propriétés de la prothrombine purifiée. Path. Biol 7: 2531 1959;
22 Tager M, Drummond M. G. Staphylocoagulase. Ann. N. Y. Acad. Sci 128: 92 1965;