Platelet Aggregation by Magnesium Ion

R. M Des Prez; R. E Bryant; J. A Katz; Th. E Brittingham

doi:10.1055/s-0038-1654176

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1967; 17(03/04): 516-531
DOI: 10.1055/s-0038-1654176

Originalarbeiten - Original Articles - Travaux Originaux

Schattauer GmbH

Platelet Aggregation by Magnesium Ion

Authors

R. M Des Prez M. D.,

¹Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee
R. E Bryant M. D.,

¹Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee
J. A Katz

¹Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee
Th. E Brittingham M. D.

¹Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee

Abstract

Summary

Washed rabbit platelets resuspended in plasma anticoagulated with the disodium magnesium salt of EDTA (magnesium EDTA) aggregate spontaneously. Analysis of this phenomenon has demonstrated that this aggregation is caused by the slightly elevated concentrations of magnesium present in plasmas anticoagulated with this agent. Addition of magnesium ion in appropriate concentrations to platelet-rich-plasma anticoagulated with 0.38% sodium citrate or 0.1% disodium dihydrogen EDTA (sodium EDTA) also produces gross clot formation, an effect which does not occur when very slightly higher concentrations of sodium EDTA are employed. The present studies distinguish between the effects of calcium ion and magnesium ion in the production of both platelet aggregation and clot formation in several commonly employed anticoagulant solutions. They demonstrate that platelet aggregation is produced by magnesium ion alone, that this aggregation is not associated with release of platelet serotonin, and that it is not caused by ADP.

Full Text

References

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