Thrombosis and Haemostasis, Inhaltsverzeichnis Thromb Haemost 1960; 4(03): 365-368DOI: 10.1055/s-0038-1654519 Originalarbeiten — Original Articles — Travaux Originaux Schattauer GmbH Observations on the Handling of Bovine Prothrombin and Thrombin[*] Autor*innen Institutsangaben Diethard F. Steichele 1 Department of Physiology and Pharmacology, Wayne State University, College of Medicine Detroit, Michigan, USA Eberhard F. Mammen 1 Department of Physiology and Pharmacology, Wayne State University, College of Medicine Detroit, Michigan, USA Yoshio Hatta 1 Department of Physiology and Pharmacology, Wayne State University, College of Medicine Detroit, Michigan, USA Walter H. Seegers 1 Department of Physiology and Pharmacology, Wayne State University, College of Medicine Detroit, Michigan, USA Artikel empfehlen Abstract Artikel einzeln kaufen(opens in new window) Summary In the handling of thrombin activity is lost by freeze drying, and by dialysis of dilute solutions. Acetone drying can be done without loss of activity. Acetylated thrombin does not lose activity during freeze drying. Prothrombin activity is retained during freeze drying and during dialysis, but is lost with acetone drying and when dilute solutions are placed in ordinary glassware that is not lined with paraffin. Volltext Referenzen References (1) Seegers W. H. The Purification of Prothrombin. Record of Chem. Progr. 13: 143 1952; (2) Miller K. D. Chromatographie Isolation of Plasma Prothrombin and Trans-α-Glucosylase. J. biol. Chem. 231: 987 1958; (3) Seegers W. H, Casillas G, Shepard R. S, Thomas W. R, Halick P. Some Properties of Thrombin Preparations. Canad. J. Biochem. 37: 775 1959; (4) Seegers W. H, Smith H. P. Factors which Influence the Activity of Purified Thrombin. Amer. J. Physiol. 137: 348 1942; (5) Sherry S, Troll W. The Action of Thrombin on Synthetic Substrates. J. biol. Chem. 208: 95 1954; (6) Landaburu R. H, Seegers W. H. The Acetylation of Thrombin. Canad. J. Biochem. 37: 1361 1959; (7) Waugh D. F, Livingstone B. J. Kinetics of the Interaction of Bovine Fibrinogen and Thrombin. J. Physiol. and Colloid Chem. 55: 1206 1951; (8) Seegers W. H, Miller K. D, Andrews E. B, Murphy R. C. Fundamental Interactions and the Effect of Storage, Ether, Adsorbants and Blood Clotting on Plasma Antithrombin Activity. Amer. J. Physiol. 169: 700 1952;
