Summary
Heparinized blood or plasma coagulates if, after addition of oxalate, recalcification
follows. Of the decalcifying agents only oxalate ion has been suitable for demonstrating
this phenomenon. Oxalate seem to accomplish two different roles connected with this
effect: a fundamental one, i. e., to sensitize a heparinlipoprotein complex to the
action of an anti-heparin factor found in normal plasma or serum and a secondary one,
related to its capacity to adsorb this antiheparin factor. The latter is removable
by centrifugation. This anti-heparin oxalate factor, which is able to counteract the
action of heparin after previous addition of oxalate, was found in sequestrened, Dowex
50 resin plasma or in serum, but is not active in citrated plasma. This factor was
removed from plasma by adsorption with barium sulfate, aluminium hydroxide or calcium
oxalate and was eluted from these adsorbants after incubation with saline.
