Purified Platelet Cofactor I and Purified Autoprothrombin II in Thromboplastin Generation Test^[*]

Summary

Purified bovine platelet cofactor I substituted for adsorbed plasma in the thromboplastin generation test when Ac-globulin was also supplied. Only 70 gamma/ml were needed. Purified bovine autoprothrombin II substituted for serum in the test. Only 9 gamma were equivalent to serum. Platelet cofactor I thus takes the place of the antihemophilic factor and autoprothrombin II substitutes for PTC and Stuart-Prower factor. We regard the autoprothrombin II preparation used as a single component derived from prothrombin.

^* This investigation was supported by a research grant H-2026 from the National Heart Institute, National Institutes of Health, U. S. Public Health Service.

• References

• 1 Seegers H, Alkjaersig N, Johnson S. A. Formation of Autoprothrombin in Solutions Containing Purified Prothrombin and Purified Platelet Factor 3. Amer. J. Physiol. 181: 589 1955;
• 2 Seegers W. H, Landaburu R. H, Fenichel R. L. Isolation of Platelet Cofactor I From Plasma and Some Properties of the Preparation. Amer. J. Physiol. 190: I 1957;
• 3 Seegers W. H, Mammen E. F, Lee J. M, Landaburu R. H, Cho M. H, Baker W. J, Shepard R. S. Further Studies on the Purification of Platelet Cofactor I. In Hemophilia and other Hemorrhagic States. Edited by Brinkhous K. M. Univ. of North Carolina Press; Chapel Hill: 1959: 38.
• 4 Shulman S, Landaburu R. H, Seegers W. H. Biophysical Studies on Platelet Cofactor I Preparations. Thromb. Diath. haem. 04: 336 1960;
• 5 Seegers W. H, Johnson S. A. Conversion of Prothrombin to Autoprothrombin II (Platelet Cofactor II) and its Relation to the Blood Clotting Mechanisms. Amer. J. Physiol. 184: 259 1956;
• 6 Mammen E. F, Thomas W. R, Seegers W. H. Activation of Purified Prothrombin to Autoprothrombin I or Autoprothrombin II (Platelet Cofactor II) or Autoprothrombin II-A. Thromb. Diath. haem. 05: 218 1960;
• 7 Biggs R, Douglas A. S. The Thromboplastin Generation Test. J. Clin. Path. 06: 23 1953;
• 8 Biggs R, Douglas A. S, Macfarlane R. G. The Formation of Thromboplastin in Human Blood. J. Physiol. 119: 89 1953;
• 9 Biggs R, Macfarlane R. G. Human Blood Coagulation and Its Disorders. 2nd Edition. Blackwell; Oxford: 1957
• 10 Ulutin O. N, Karaca M, Sestakof D. Thromboplastin Husul Testi (Thromboplastin Generation Test), ve klinik önemi. Türk Tip Cem. Mec. 24: 527 1958;