The Quantitation of Platelet Aggregation Induced by Four Compounds: A Study in Relation to Myocardial Infarction

J. R O’Brien; F. C Path; Joan B. Heywood; J. A Heady

doi:10.1055/s-0038-1655660

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1966; 16(03/04): 752-767
DOI: 10.1055/s-0038-1655660

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

The Quantitation of Platelet Aggregation Induced by Four Compounds: A Study in Relation to Myocardial Infarction

Autor*innen

J. R O’Brien M. A., D. M

¹From the Portsmouth and Isle of Wight Pathological Service and Social Medicine Research Unit of the Medical Research Council, The London Hospital
F. C Path

¹From the Portsmouth and Isle of Wight Pathological Service and Social Medicine Research Unit of the Medical Research Council, The London Hospital
Joan B. Heywood B. Sc

¹From the Portsmouth and Isle of Wight Pathological Service and Social Medicine Research Unit of the Medical Research Council, The London Hospital
J. A Heady Ph. D.

¹From the Portsmouth and Isle of Wight Pathological Service and Social Medicine Research Unit of the Medical Research Council, The London Hospital

Abstract

Summary

Methods for measuring and comparing day to day differences in the response of platelet aggregation in platelet-rich plasma to added ADP, 5-H.T., adrenaline and collagen are reported. Platelet aggregation induced by ADP, 5-H.T. and adrenaline was studied in patients with acute myocardial infarction and in others 3 months to 5 years after an infarct; some were receiving anti-coagulants and others not: these three groups were compared with three control groups. The mean platelet shape was rounder and the response to ADP and to 5-H.T. and one parameter of the response to adrenaline was significantly greater in all groups of patients with myocardial infarct taken together than in the controls. The platelet-rich plasma from patients with recent infarction were most responsive to ADP and 5-H.T. immediately after the infarct. Anti-coagulants had no effect on these tests. However, there was wide variation within the individuals and much overlap between groups, and these tests can only reliably distinguish between groups and not between individuals. The significance of these findings is discussed.

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Referenzen

References
1 Born G. V. R. Aggregation of Blood Platelets by Adenosine Diphosphate and its Reversal. Nature (Lond.) 194: 927 1962;
2 Emmons P. R, Mitchell J. R. A. Post-Operative Changes in Platelet-Clumping Activity. Lancet 1: 71 1965;
3 Feissly R, Lüdin H. Microscopie par Contrastes de Phases. Rev. Hémat 4: 481 1949;
4 Gaarder A, Jonsen J, Laland S, Hellem A, Owren P. A. Adenosine Diphosphate in Red Cells as a Factor in the Adhesiveness of Human Blood Platelets. Nature (Lond.) 192: 531 1961;
5 McGlure P. D, Ingram G. I. G, Vaughan Jones R. Platelet Changes After Adrenaline Infusions with and without Adrenaline Blockers. Thrombos. Diathes. haemorrh 13: 136 1965;
6 McDonald L, Edgill M. Coagulability of the Blood During Various Phases of Ischaemic Heart Disease. Lancet 1: 1115 1959;
7 Mitchell J. R. A, Sharp A. A. Platelet Clumping in Vitro. Brit. J. Haemat 10: 78 1964;
8 Moolten S. E, Jennings P. B, Solden A. Dietary Fat and Platelet Adhesiveness in Arteriosclerosis and Diabetes. Amer. J. Cardiol 11: 290 1963;
9 O’Brien J. R. Platelet Aggregation, Part II, Some Results from a New Method of Study. J. clin. Path 15: 452 1962;
10 O’Brien J. R. Variability in the Aggregation of Human Platelets by Adrenaline. Nature (Lond.) 202: 1188 1964;
11 O’Brien J. R. A Comparison of Platelet Aggregation Produced by Seven Compounds and a Comparison of Their Inhibitors. J. clin. Path 17: 275 1965;
12 O’Brien J. R. Effects of Adenosine Diphosphate and Adrenaline on the Mean Platelet Shape. Nature (Lond.) 207: 306 1965;
13 Wright H. P. Changes in the Adhesiveness of Blood Platelets Following Parturition and Surgical Operations. J. Path. Bact 54: 461 1942;