Summary
The absorption spectrum and Polarographie behaviour are described for phenylindanedione.
The drug may be estimated by measuring the optical density at 338 nιμ or the Polarographie
reduction ware in a phosphate buffer at pH 11.8. For the estimation of the drug in
tissue and plasma, the substance is extracted with toluene, taken up in alkali and
estimated spectrometrically. For red cells, it is necessary to extract with acetone
and after evaporation, take up the residue in water and determine the phenylindanedione
polarographically. Blood and tissue levels were determined after administration to
rabbits and dogs. Phenylindanedione disappeared from the plasma after intravenous
injection much more rapidly than dicumarol. Considerable amounts of the drug were
detected in liver and intestine one hour later. On oral administration, after 4—6
hours 50°/o was still recovered from the intestine, while 10—20% was found in the
liver. Phenylindanedione and / or its metabolic derivative could be detected in the
erythrocytes of rabbits but not in those of dogs.
