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Thromb Haemost 1958; 02(03/04): 236-249
DOI: 10.1055/s-0038-1656276
Originalarbeiten — Original Articles — Travaux Originaux
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Distribution of Phenylindanedione in Blood and Tissues after Oral and Intravenous Administration

G. J Millar
1   Dept. of Physiology and Pharmacology, University of Saskatchewan, Saskatoon (Canada)
,
Mary Ogilvie Mersereau
1   Dept. of Physiology and Pharmacology, University of Saskatchewan, Saskatoon (Canada)
,
J Lowenthal
1   Dept. of Physiology and Pharmacology, University of Saskatchewan, Saskatoon (Canada)
,
L. B Jaques
1   Dept. of Physiology and Pharmacology, University of Saskatchewan, Saskatoon (Canada)
› Author AffiliationsSupported by a grant-in-aid from the Medical Division of the National Research Council, of Canada. We are also indebted to Chas. E. Frosst & Co. for financial aid and for the phenylindanedione.
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Publication Date:
26 July 2018 (online)

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Summary

The absorption spectrum and Polarographie behaviour are described for phenylindanedione. The drug may be estimated by measuring the optical density at 338 nιμ or the Polarographie reduction ware in a phosphate buffer at pH 11.8. For the estimation of the drug in tissue and plasma, the substance is extracted with toluene, taken up in alkali and estimated spectrometrically. For red cells, it is necessary to extract with acetone and after evaporation, take up the residue in water and determine the phenylindanedione polarographically. Blood and tissue levels were determined after administration to rabbits and dogs. Phenylindanedione disappeared from the plasma after intravenous injection much more rapidly than dicumarol. Considerable amounts of the drug were detected in liver and intestine one hour later. On oral administration, after 4—6 hours 50°/o was still recovered from the intestine, while 10—20% was found in the liver. Phenylindanedione and / or its metabolic derivative could be detected in the erythrocytes of rabbits but not in those of dogs.

 

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