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Hamostaseologie 1999; 19(03): 134-138
DOI: 10.1055/s-0038-1660401
Übersichts- und Originalarbeiten/Review and Original Articles
F. K. Schattauer Verlagsgesellschaft

A Simple and Rapid Method to Determine GPIIb/IIIa-Receptor Inhibitor Activity in Whole Blood

A. C. Matzdorff
1   Dept. of Hematology/Oncology (Chair: Prof. Dr. H. Pralle), Center for Internal Medicine, Justus-Liebig-University, Giessen, Germany
,
Gitta Kühnel
1   Dept. of Hematology/Oncology (Chair: Prof. Dr. H. Pralle), Center for Internal Medicine, Justus-Liebig-University, Giessen, Germany
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Publication History

Publication Date:
22 July 2018 (online)

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Summary

We studied the effect of GPIIb/IIIa-inhibitors on platelet activation with flow cytometry in vitro. Citrated whole blood was incubated with increasing concentrations of three different GPIIb/IIIa-inhibitors (c7E3, DMP728, XJ757), then thrombin or ADP were added and after 1 min the sample was fixed. Samples without c7E3 but with 0.1 U/ml thrombin had a decrease in platelet count. Samples with increasing concentrations of c7E3 had a lesser or no decrease in platelet count. The two other inhibitors (DMP 725, XJ757) gave similar results. GPIIb/IIIa-inhibitors prevent aggregate formation and more single platelets remain in the blood sample. The agonist-induced decrease in platelet count correlates closely with the concentration of the GPIIb/IIIa inhibitor and receptor occupancy. This correlation may be used as a simple measure for inhibitor activity in whole blood.

Keywords

Platelet activation - GPIIb/IIIa-inhibitors - flow cytometry
 

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