In Vitro Effects of Dihomo-γLinolenic Acid (DHLA) on Normal and Diabetic Platelet Function

DHLA causes a general inhibition of platelet reactions in standard in vitro tests of platelet function. Significantly more DHLA is required for the 50% inhibition (ID ₅₀) of diabetic when compared to normal platelet reactions (Kernoff et al. Thrombosis & Haemostasis 38, 194, 1977). To investigate the cause of this difference we have studied kinetics of ADP-induced primary platelet aggregation, its inhibition by DHLA, and the formation of malondialdehyde (MDA) in the presence of DHLA in platelet-rich plasma of six healthy subjects and six diabetic patients with advanced microangiopathy. The results showed a significantly lower Km (ADP) for platelet aggregation in the diabetic group compared to normal. The (DHLA) of the competitive component of inhibition of platelet aggregation (prostaglandin production-mediated) was not significantly different In the two groups. Also, amounts of MDA formed in diabetic and normal PRP in the presence of DHLA were not significantly different. We conclude that the apparent low susceptibility of diabetic platelets to inhibition by DHLA might be a result of a primary hyper-reactivity of these platelets due to a cause other than an abnormality of the platelet PG production pathway.

 

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