Tierarztl Prax Ausg K Kleintiere Heimtiere 2019; 47(03): 217
DOI: 10.1055/s-0039-1688640
Posterpräsentationen
Experimentelle Pathologie
Georg Thieme Verlag KG Stuttgart · New York

Neuroprotective function of CARD9 signaling in acute Theiler's murine encephalomyelitis infection

S Pavasutthipaisit
1   Department of Pathology
3   Center for Systems Neuroscience, Hannover, Germany
4   Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand
,
M Stoff
1   Department of Pathology
,
M Ciurkiewicz
1   Department of Pathology
3   Center for Systems Neuroscience, Hannover, Germany
,
T Störk
1   Department of Pathology
,
S Mayer-Lambertz
2   Research Center for Emerging Infections and Zoonoses, Immunology Unit, University of Veterinary Medicine Hannover, Germany
,
T Ebbecke
2   Research Center for Emerging Infections and Zoonoses, Immunology Unit, University of Veterinary Medicine Hannover, Germany
3   Center for Systems Neuroscience, Hannover, Germany
,
W Baumgärtner
1   Department of Pathology
3   Center for Systems Neuroscience, Hannover, Germany
,
B Lepenies
2   Research Center for Emerging Infections and Zoonoses, Immunology Unit, University of Veterinary Medicine Hannover, Germany
3   Center for Systems Neuroscience, Hannover, Germany
,
A Beineke
1   Department of Pathology
3   Center for Systems Neuroscience, Hannover, Germany
› Author Affiliations
Further Information

Publication History

Publication Date:
18 June 2019 (online)

Also available at
 
 

    Introduction:

    Theiler's murine encephalomyelitis virus (TMEV) infection of wild type (C57BL/6) mice represents an animal model for hippocampal degeneration and epilepsy. Caspase recruitment domain family member 9 (CARD9) is a signaling protein which plays a role in inflammation and immunity. So far, the effect of CARD9 signaling in viral encephalitis remains undetermined.

    Materials and methods:

    CARD9-/- and C57BL/6 (wild type control) mice were intracerebrally inoculated with TMEV. Brain tissue was investigated by histology and immunohistochemistry. Spleen tissue was analyzed by flow cytometry.

    Results:

    Results showed an enhanced brain inflammation at 7 dpi in CARD9-/- mice compared to wild type mice. In addition, the number of beta-amyloid precursor protein-positive axons was significantly increased in the hippocampus of CARD9-/- mice at 7 and 14 dpi. T cell activation was significantly increased in CD4+ and CD8+ T cells in CARD9-/- mice at 14 dpi as indicated by CD62L down-regulation.

    Conclusion:

    CARD9 deficiency causes axonal damage in seizure-prone C57BL/6 mice following TMEV infection, indicating a neuroprotective function of intact CARD9 signaling in acute viral infection.


    #