PDF icon Download PDF
Thromb Haemost 1975; 34(01): 345
DOI: 10.1055/s-0039-1689166
Abstracts
Schattauer GmbH

Coagulation Inhibitors Studied with Chromogenic Substrate

U. Abildgaard
1   Medical Department A, Aker Hospital, Oslo 5, Norway
,
M. Lie
1   Medical Department A, Aker Hospital, Oslo 5, Norway
,
A. Teien
1   Medical Department A, Aker Hospital, Oslo 5, Norway
,
O. R. Ødegård
1   Medical Department A, Aker Hospital, Oslo 5, Norway
› Author Affiliations
Further Information

Publication History

Publication Date:
22 May 2019 (online)

 
 PDF Download Permissions and Reprints

    The rapid amidolysis of the chromogenic substrate Bz-Phe-Val-Arg-pNA by thrombin (Svendsen & al. Thromb. Res. 1, 267, 1972) provides a useful tool for the study of coagulation inhibitors. With purified reagents, reversible inhibition of the reaction can be demonstrated with 0.01 U/ml of heparin. The inactivation of thrombin by antithrombin III (At-III) is accelerated by 0.001 U/ml of heparin, making it possible to assay small amounts of heparin. The amidolytic assay system has also been used to study the competition between thrombin and factor Xa for At-III.

    The sensitivity and specificity of rapid amidolytic methods for assay of the following plasma activités will be reported:

    1) At-III activity (contaminating heparin neutralized).

    2) Exogenous heparin (during heparin treatment).

    3) Combined effect of At-III and exogenous heparin.

    4) At-III in the presence of optimal concentrations of heparin.


     

     


     
      Permissions and Reprints