Lymphaticovenous Anastomosis for Lower Extremity Lymphedema: A Systematic Review

Abstract

Background Lymphedema is an accumulation of protein-rich fluid in the interstitial spaces resulting from impairment in the lymphatic circulation that can impair quality of life and cause considerable morbidity. Lower extremity lymphedema (LEL) has an overall incidence rate of 20%. Conservative therapies are the first step in treatment of LEL; however, they do not provide a cure because they fail to address the underlying physiologic dysfunction of the lymphatic system. Among several surgical alternatives, lymphaticovenous anastomosis (LVA) has gained popularity due to its improved outcomes and less invasive approach. This study aims to review the published literature on LVA for LEL treatment and to analyze the surgical outcomes.

Methods PubMed database was used to perform a comprehensive literature review of all articles describing LVA for treatment of LEL from Novemeber 1985 to June 2019. Search terms included “lymphovenous” OR “lymphaticovenous” AND “bypass” OR “anastomosis” OR “shunt” AND “lower extremity lymphedema.”

Results A total of 95 articles were identified in the initial query, out of which 58 individual articles were deemed eligible. The studies included in this review describe notable variations in surgical techniques, number of anastomoses, and supplementary interventions. All, except one study, reported positive outcomes based on limb circumference and volume changes or subjective clinical improvement. The largest reduction rate in limb circumference and volume was 63.8%.

Conclusion LVA demonstrated a considerable reduction in limb volume and improvement in subjective findings of lymphedema in the majority of patients. The maintained effectiveness of this treatment modality in long-term follow-up suggests great efficacy of LVA in LEL treatment.

Keywords

lower extremity - lymphaticovenous anastomosis - lymphaticovenous bypass - lymphedema - lymphovenous anastomosis - lymphovenous bypass

Introduction

Impairment in lymphatic circulation leads to the accumulation of protein-rich fluid in the interstitial spaces, giving rise to a chronic and debilitating condition known as lymphedema that is characterized by edema, inflammation, and cellulitis. Individuals suffering from lymphedema experience pain, fatigue, numbness, swelling, depression, limitation of daily activities, social impairment, and difficulty wearing normal clothing.[1] [2] These functional and cosmetic disabilities greatly affect their quality of life. Eventually, irreversible changes ensue, including fibrosis and proliferation of adipose tissue.[2] In rare cases, continual angiogenic stimulus resulting from chronic lymphedema can manifest in the form of malignancies, such as lymphangiosarcoma (Stewart–Treves syndrome), Kaposi’s sarcoma, and lymphoma.[3]

Primary lymphedema is characterized by a disruption in normal lymphatic fluid transport due to agenesis or dysgenesis of any component of the lymphatic network.[4] [5] [6] [7] [8] Occasionally, a lymphatic thrombus may be the cause.9 Secondary lymphedema is caused by mechanical obstruction of lymphatic drainage due to trauma, infection, radiation, or surgical disruption.[3] [10] [11] [12]

Lower extremity lymphedema (LEL) has an overall incidence of 20%.[13] It is reported to occur in up to 21% of prostate, 16% of bladder, 4% of penile, 11.1% of ovarian,[14] 12.6% to 27.0% of cervical,[15] 13% of endometrial,[16] and 16.7 to 30.0% of vulvar cancers after radical surgery.[17] [18] [19] Several risk factors for LEL have been identified, including older age, higher body mass index, radiation therapy, cellulitis and wound infection, and the number of removed lymph nodes.[18] [20]

Conservative therapies do not address the underlying dysfunction of the lymphatic drainage system and, hence, are ineffective in halting the progression of the disease. Surgical techniques, reconstructive or physiologic, target the functional repair of this intrinsic problem. Among these is a supermicrosurgical procedure known as lymphaticovenous anastomosis (LVA) or lymphovenous bypass that has gained wide spread acceptance worldwide due to its improved outcomes and less invasive approach. Anastomosing lymphatic channels to the adjacent venules redirects the lymphatic flow to the venous circulation, thereby draining the excess fluid trapped in the lymphedematous district. The lymphatic vessel and venule are anastomosed in an intima-to-intima coaptation fashion, leading to a lower anastomotic-site thrombosis rate.[21] The outcome of this technique is influenced by the quality of the lymphatic vessels bypassed and the number of LVAs made. Functional smooth muscle cells lining the lymphatic channels are required to generate an effective pressure gradient to force the congested lymph into the venous system.[22]

This study aims to review the published literature on LVA in the treatment of LEL and to analyze the surgical outcomes. To our knowledge, this is the first review that includes all studies to date describing LVA surgery and its effect on primary and secondary lymphedema of lower extremities.

Methods

PubMed was queried for articles reporting LVA surgery in LEL treatment and their postsurgical outcomes using the search terms, “lymphovenous” OR “lymphaticovenous” AND “bypass” OR “anastomosis” OR “shunt” AND “lower extremity lymphedema.” Only human clinical studies in the English language were eligible for inclusion. Manuscripts mentioning both upper and lower limb lymphedema were included as long as their results included extractable data regarding outcomes of LVA for LEL treatment. Literature review articles were excluded. The publications were further screened manually and reviewed to identify additional reports for surgical treatment of LEL through LVA. The second author (N.K.) independently reviewed and extracted data from the papers according to the predetermined criteria. Demographic data, lymphedema duration, surgical technique, follow-up time, and outcomes were extracted from the selected articles.

Fig. 1 Flowchart highlighting search strategy and article identification.

Results

We found a total of 95 articles in our first search. From these, only 58 studies met inclusion criteria ([Fig. 1]). The manuscripts included were published from November 1985 to July 2019 and reported data for a total of 1,363 patients with LEL who had undergone LVA [Supplemnetary Table S1], (available online only). The number of patients in each study ranged from 1[23] [24] [25] [26] [27] to 216,[28] with a female predominance in all. The mean age at presentation ranged from 6[29] to 94 years,[30] and the mean duration of LEL ranged from 22 days[31] to 585 months.[1] Patients included in the studies more commonly had secondary lymphedema.

Campisi’s lymphedema grading system[32] was used in 12 studies, while the Yamamoto classification system[33] based on lymphatic dermal backflow patterns seen on indocyanine green lymphography (ICG-L) was used in 17 studies. Mihara et al,[34] Akita et al,[35] and Maegawa et al[36] used the grading system based on lymphoscintigraphy findings, described by Maegawa et al[37] in one of their earlier reports. Ito and collegues[38] used Cheng’s classification system[39] and Olszewski[28] used a grading system based on evaluation of the level of edema embracing the limb from foot to groin and the advancement of skin keratosis and fibrosis. However, the most widely used classification in 20 studies was the one presented by the International Society of Lymphology.[40]

The preoperative and operative details along with the outcomes are summarized in [Supplementary Table S2] (available online only). Lower limb circumference and volume change were the most frequently assessed parameters, followed by subjective improvement. Mihara et al[30] and Gloviczki et al[41] used lymphoscintigraphy, while Chen and colleagues used ICG-L[42] to assess postoperative efficacy of LVA. Akita et al[43] used computed tomography to calculate thickness of the subcutaneous fat layer on the lateral edge of the rectus femoris muscle at the level of the lesser trochanter of the femur and subsequently evaluated postoperative improvement of lymphedema.

ICG-L was the most commonly used investigation to assess the severity of lymphedema and preoperatively outline the location of lymphatic vessels. End-to-end (E-E) and side-to-end (S-E) anastomosis were the most frequently employed surgical techniques. A modified version of the E-E LVA, the sleeve-in technique, in which a lymphatic vessel is implanted into the lumen of a vein, was used by Yamamoto and Sugihara,[44] Olszewski,[28] and Chung et al.[45]

Yamamoto et al[46] introduced the l-shaped anastomosis with intravascular stent (IVaS) method for cases in which only one lymphatic channel and one venule could be found. They suggested identifying vessels appropriate for anastomosis and transecting them. After that, an IVaS, a piece of nylon monofilament suture, is placed in the vessels to keep the lumen open and assist safe anastomosis. An end-to-side (E-S) LVA followed by an E-E LVA is performed, creating a l-shaped anastomosis.[46] Furthermore, when only one venule and two lymphatic channels were found, they reported the use of sequential anastomosis, consisting of S-S and S-E LVA. Four directions of lymphatic flow can be diverted from two lymphatic vessels into one venule via this arrangement.[47] They also described the use of ladder-shaped anastomosis for cases when only one vein and three lymphatic vessels were identified intraoperatively.[48] In this technique, the two lymphatic vessels next to the vein are anastomosed to the vein in a S-S fashion, and the other lymphatic vessel is anastomosed to the nearby lymphatic vessel in a S-S fashion.

Ayestaray and Bekara[49] developed a configuration based on two E-S LVA, or π-shaped LVA. They called it a venous flow-sparing technique that resulted in an average circumferential differential reduction rate of 19.4% (range, 6.1–24.6%).[49] In an attempt to solve the problem of mismatch between the number of lymphatic vessels and venules and their calibers, Chen et al[50] presented the octopus LVA. This technique allows multiple lymphatic vessels to be bypassed using a single vein. An out-to-in transluminal suture is placed through a vein with a large diameter, followed by an in-to-out transluminal suture, which intussuscepts all the lymphatic vessels into the vein, and forms an LVA complex that resembles an octopus.[50]

Per case 1[29] [30] [36] [51] [52] [53] to 18[46] anastomoses were performed, and it took an average of 3 to 4 hours[29] [35] [44] [45] [46] [54] [55] [56] [57] [58] for surgeons to perform the entire procedure. Follow-up time ranged from 1[59] [60] to 87 months.[61] Complex decongestive physical therapy was advised to be continued postoperatively in most cases. All studies reported positive outcomes based on improvement in the limb circumference and volume, lymphatic function, or clinical symptoms. Ito et al,[38] Koshima et al,[62] and Lee et al[63] have reported the largest reduction rates at 63.8, 55.6, and 51.1%, respectively.

Regarding outcomes, Gloviczki and collegues[41] reported improvement in only two of eight patients, while four patients reported worsening of lymphedema. Mihara et al[34] compared LVA outcomes in the following two groups of patients: those in whom noncontact vein visualizing system was used to detect subcutaneous veins (control group) versus those in whom venous echography was used (echo group). Lymphedema exacerbation was reported in 23.8% of the patients from the control group, while only 5.9% from the echo group experienced lymphedema deterioration.[34] In patients with lymphedema onset before 11 years of age, Hara et al[58] reported aggravation of their condition. Positive outcomes were seen more often in those with onset after 11 years of age.[58]

The largest number of patients included in a study was reported by Olszewski.[28] Over the course of 45 years, the author performed LVAs on 216 patients, with an average postoperative follow-up of 60 months. Major improvement was observed in patients with early stages of lymphedema, regardless of the etiology. The lymphovenous shunt operation was ineffective in patients with stage-II idiopathic and stage-III postinflammatory types of lymphedema. Hence, Olszewski suggested performing the surgery at an early stage (I and II) of postinflammatory, postsurgical, or hyperplastic types, with at least one thigh lymphatic and inguinal or iliac lymph node visible on stress lymphoscintigraphy.

Similarly, Demirtas et al[60] reported a greater decrease in limb volume in patients with a lower volume of edema. However, Mihara et al[30] described contradicting results, with significant volume reductions in advanced stages of lymphedema. They recorded a volume decline in 73.9% of patients with stage III compared with 56.7% with stage I.

Thirteen studies assessed the relationship between lymphedema and episodes of cellulitis or lymphangitis.[1] [24] [27] [29] [30] [34] [38] [56] [60] [61] [63] [64] [65] All, except one chart review by Lee et al,[63] reported significant reductions in infection incidence.

Discussion

The possibility of creating an anastomosis between lymphatic vessels and veins to diverge the lymphatic fluid into the venous system was mentioned for the first time in 1962.[66] Subsequently, several others attempted to perform LVA in canine models.[67] [68] [69] However, the field of lymphatic microsurgery did not experience a major breakthrough until O’Brien et al[70] presented a clinical report establishing the clinical effectiveness of this method in human patients. Lymphatic vessels with a diameter of 0.5 to 1.0 mm and veins with a diameter of 2.0 to 3.0 mm were required for anastomosis under general anesthesia.[70] Thereafter, the introduction of supermicrosurgery, which enabled anastomosis of vessels with a caliber of 0.3 to 0.8 mm, increased the reliability of LVA.[62] [71] LVA using lymphatics with diameter between 0.5 to 0.7 mm and subdermal veins between 0.7 to 1.0 mm of diameter demonstrated positive outcomes with reduction rates from 30 to 78% and reduction of episodes of cellulitis from a mean of 1.6 to 0.2.[38] Currently, the operation can be performed under local anesthesia in the dermal layer of the skin via a 2- to 3-cm skin incision, making it safe for high-risk patients, including the elderly and those with cardiopulmonary disease or terminal cancer.[62] Mihara et al[72] was able to report favorable outcomes of LVA performed through a 2-mm skin incision under guidance of the AccuVein system (AccuVein Inc.) and ICG-L in patients with early and latent stage lymphedema. This was possible because of the absence of subcutaneous tissue fibrosis in these patients.

LVA is indicated in cases where there has been insufficient lymphedema reduction by complex physical therapy (less than 50%), worsening limb function, recurrent episodes of cellulitis, and intractable pain. In addition, patients not satisfied with the result obtained by conservative methods can be given the option to proceed with a surgical alternative.[73] LVA can also be performed as prophylactic treatment in patients with subclinical lymphedema.[72] [74]

Many surgeons agree that it is desirable to perform microlymphatic surgery at an early stage of edema because lymphatics get permanently damaged from increased pressure and recurrent infections, and preserved smooth muscle function is required to effectively pump the lymphatic fluid into the venous system.[32] [35] [38] [53] [60] [61] [65] [71] [75] [76] [77] Thus, advanced disease, where lymphatic vessels are usually sclerotic, is a relative contraindication.

Other procedures used to treat lower limb lymphedema include vascularized lymph node transfer (VLNT), as well as VLNT in combination with lipoaspiration.[78] Although, VLNT is also preferred in early stages of the disease as LVA, LVA is better suited in patients with available lymphatic vessels.[78] [79] [80] In addition, LVA has the advantage of not having any morbidity risk associated with the donor site as in VLNT.[79] On the other hand, combined procedures have shown better outcomes in more advanced stages of lymphedema.[78]

Previously, it was believed that primary lymphedema was also a relative contraindication for LVA due to the hypoplastic nature of lymphatic vessels.[75] However, encouraging results have been reported for select patients with primary lymphedema in the recent years.[58] [81] Congenital lymph node fibrosclerosis and the lower number of functional lymphatic collectors can account for the decreased volume reduction observed in primary lymphedema as compared with secondary.[32] [81] Since extensive lymphatic abnormalities are associated with early onset of disease, LVA is advised in cases of primary lymphedema with no dermal backflow and disease onset after 11 years of age.[58]

Regarding the surgical technique, subdermal venules with intact valves are recommended for anastomosis due to their small diameter and lower intravenous pressures. The smaller diameter allows caliber-matching, and lower intravenous pressure with a functioning valve reduces the chance of thrombosis due to minimal backflow.[82]

Constant lymphatic vessels are easier to locate at the dorsal foot, ankle, and groin region for the lower extremity.[29] However, Seki et al[83] described a method to locate easily between three to five superficial large lymphatic vessels when a 2.5-cm transverse incision is made in the intersection of a transverse line at the superior edge of the patella and a longitudinal line along the medial axis of the distal thigh. Preoperative identification of functional lymphatic vessels and veins can substantially shorten operative time and improve surgical outcomes.[84] For this purpose, ICG-L and lymphoscintigraphy can be used to locate patent lymphatic vessels and assess the severity of lymphedema before surgery.[55] [56] [72] [85] [86] [87] Although, our systematic review observed different classifications used to assess severity of lymphedema, the most widely used classification was the one stated by International Society of Lymphology. Ultrasound is a noninvasive tool that can also facilitate lymphatic channel visualization in regions masked by dermal backflow pattern or in patients allergic to iodine.[55] [57] It has a sensitivity and specificity of 88.2 and 92.7%, respectively.[57] Postoperative patency and efficacy of the anastomosis can also be determined via lymphoscintigraphy[41] and ICG-L.[42] Lee et al[63] published a chart review in which they suggested that once LVA is conducted properly and becomes functional in the early postoperative period, it might remain effective for up to 4 years following surgery.

Performing as many LVAs as possible is desirable, given that treatment efficacy was found to be proportional to the number of anastomoses created.[38] [46] [65] [82] [86] However, there is still a topic open for investigation regarding the number of anastomoses that result in significant reductions. Chen[88] in his operative technique that routinely they perform between 7 to 12 per limb. Koshima et al[53] believe that a large number of LVAs is not required, and only two to three anastomoses are enough to provide a satisfactory volume reduction. Seki and colleagues, on the other hand, commented that one functional LVA can be sufficient.[83]

The basic types of LVA include E-E, E-S, S-E, and S-S.[46] [47] [52] [89] [90] [91] Controversy still exists regarding the type of anastomosis that should be performed but each type has its own advantages and disadvantages. E-E LVA seems to be inferior to E-S and S-S LVA as it drains only distal lymph in LEL and requires a higher number of anastomoses in more proximal sites.[92] Moreover, lymphedema is characterized to have a retrograde and antegrade lymph flow, therefore, LVA should try to bypass both directions of lymph flows.[92] S-E LVA is the most technically challenging and recommended, and is efficient to divert bidirectional lymph flow via one anastomosis.[38] Similarly, S-S anastomosis can divert both antegrade and retrograde lymph flows, but it takes a longer time compared with S-E anastomosis and eventually results in venous backflow, which may cause anastomotic site thrombosis. However unlike S-E LVA, S-S LVA does not require supermicrosurgical technique when the diameter of the lymphatic vessel is smaller than 0.5 mm, can be performed when the vein is much larger than the lymphatic vessel, and can use a vein distal to the anastomotic site.[37] [46] [47] [86] [90]

Due to the small caliber of microvessels and the transparent nature of lymphatic vessels, the procedure of LVA is technically challenging. Yamamoto et al[90] proposed the method of temporary lymphatic expansion during S-E anastomosis, whereby the lymphatic vessels are clamped and manually massaged. Although temporary, this maneuver can dilate the vessels by approximately 0.12 mm, making the creation of a lateral window much easier. Another way to facilitate the anastomosis is by inserting an IVaS, a piece of nylon suture that allows identification of the lumen of lymph channels and stabilizes vessels while guiding the needle.[46] [91] [93] Building on this approach, Yoshida et al[54] illustrated the mechanical dilatation technique, in which a larger nylon monofilament was inserted after the IVaS to dilate the vessel lumen. According to their data, even a vessel with a diameter of less than 0.1 mm can be dilated to more than 0.2 mm using this method, with 100% immediate patency rate.[54] These techniques can aid in the creation of safe and secure LVAs in a shorter amount of time, thus increasing the number of successful LVAs that can be performed.

Supportive use of complex decongestive physical therapy should not be ignored after surgery.[75] [81] It consists only of compression therapy with the help of bandaging and elastic stockings but also of manual lymph drainage and massage, therapeutic exercise, and careful skin care.[29] Superior outcomes have been observed in patients who underwent a combination of LVA and compression bandaging compared with those who underwent surgery alone.[43] Compression therapy should be initiated one month postsurgery and patients should be follow up each 3 months postoperatively to assess volume reduction until maintenance of limb size is achieved with daytime therapy for 3 consecutive months.[94]

Our systematic review found that the largest reduction rates achieved after LVA for LEL ranged between 51.1 and 63.8%, with better results presented in early stages of lymphedema, and that almost all studies reported a decrease in episodes of infection. Patients’ satisfaction after LVA has been also evaluated. Chung et al[45] found moderate scores of satisfaccion after treatment. Moreover, improvement in the quality of life has been demonstrated after LVA during the first postoperative months.[1] [42] Although, most of the patients who underwent LVA for LEL have been reported to have positive surgical outcomes, limb size reduction can be variable. This may be related with the severity of the lymphedema and the number and type of anastomoses performed along with the risk factors associated with lymphedema presented in the patients included in the studies. Several studies have reported inferior outcomes for patients who have undergone LVA for LEL compared with upper extremity lymphedema.[38] The effects of ambulation, the large size, the dependent position, and the higher venous pressure of the lower extremities may account for this finding.[29] When a volumetric decrease of the edema was not achieved, it constitutes a failure. In those cases, options such as VLNT, lipoaspiration or combined procedures could be considered.

Limitations

As with all systematic reviews, this study has several limitations. Due to the general lack of large volume studies addressing LVA in the lower extremity, we were restricted to a limited range of reported data, and hence, a thorough statistical analysis was not possible. Considerable heterogeneity exists among the reported outcomes in each study, and therefore, there is a potential for bias in interpreting data, as it is possible that not all studies captured reliable comorbidity data or outcomes over a long-period of time. A risk of selective reporting bias can also be encountered when documentation of subjective patient symptoms is involved. Larger, randomized, multicenter studies are warranted to validate the results found from this systematic review of the literature.

Conclusion

The results of this systematic review of the literature on LVA for LEL demonstrated considerable improvements in objective and subjective findings in the majority of patients. More importantly, the effectiveness of this treatment modality was maintained in the long-term follow-up, suggesting great efficacy of LVA in cases of LEL.

Conflict of Interest

A.J.F. reports others and this study was supported in part by the Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, and by the Mayo Clinic Center for Individualized Medicine. D.B. reports others and This study was supported in part by the Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, and by the Mayo Clinic Center for Individualized Medicine. H.Y.S. reports others and this study was supported in part by the Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, and by the Mayo Clinic Center for Individualized Medicine. M.T.H. reports others and this study was supported in part by the Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, and by the Mayo Clinic Center for Individualized Medicine. N.K. reports others and this study was supported in part by the Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, and by the Mayo Clinic Center for Individualized Medicine. O.J.M. reports others and this study was supported in part by the Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, and by the Mayo Clinic Center for Individualized Medicine. P.C. reports others and this study was supported in part by the Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, and by the Mayo Clinic Center for Individualized Medicine. S.A.McL. reports others and this study was supported in part by the Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, and by the Mayo Clinic Center for Individualized Medicine. X.L. reports others and This study was supported in part by the Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, and by the Mayo Clinic Center for Individualized Medicine.

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Publication History

Article published online:
17 April 2020