Tierarztl Prax Ausg K Kleintiere Heimtiere 2020; 48(03): 216
DOI: 10.1055/s-0040-1712568
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Klein- und Heimtiere

Oxidative stress in canine histiocytic sarcoma cells (DH82 cells) induced by a persistent canine distemper virus infection leads to impairment of the HIF-1α downstream pathway in vitro

F. Armando
1   Department of Pathology, University of Veterinary Medicine Hannover, Germany
3   Department of Veterinary Medicine, Pathology Unit, University of Parma, Parma, Italy
,
M. Gambini
1   Department of Pathology, University of Veterinary Medicine Hannover, Germany
4   Dipartimento di Medicina Veterinaria (DIMVET), Universitá degli Studi di Milano, Lodi, Italy
,
A. Corradi
3   Department of Veterinary Medicine, Pathology Unit, University of Parma, Parma, Italy
,
C. Giudice
4   Dipartimento di Medicina Veterinaria (DIMVET), Universitá degli Studi di Milano, Lodi, Italy
,
V. M. Pfankuche
1   Department of Pathology, University of Veterinary Medicine Hannover, Germany
2   Center for Systems Neuroscience, University of Veterinary Medicine Hannover, Hannover, Germany
,
G. Brogden
5   Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Germany
,
F. Attig
1   Department of Pathology, University of Veterinary Medicine Hannover, Germany
2   Center for Systems Neuroscience, University of Veterinary Medicine Hannover, Hannover, Germany
,
M. von Köckritz-Blickwede
5   Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Germany
6   Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Germany * These authors contributed equally.
,
W. Baumgärtner
1   Department of Pathology, University of Veterinary Medicine Hannover, Germany
2   Center for Systems Neuroscience, University of Veterinary Medicine Hannover, Hannover, Germany
,
C. Puff
1   Department of Pathology, University of Veterinary Medicine Hannover, Germany
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    Introduction Histiocytic sarcoma (HS) is a highly aggressive neoplasm with a limited response to therapies. A promising new approach might be oncolytic virotherapy. Angiogenesis is essential for tumor growth and metastasis. One of the key regulator factors for angiogenesis is hypoxia inducible factor-1α (HIF-1α). In addition, angiogenesis is regulated by reactive oxygen species (ROS). The aim of this study was to evaluate the influence of canine distemper virus (CDV) infection on HIF-1α expression in canine HS cells (DH82 cells).

    Materials and methods Non-infected and persistently CDV infected DH82 cells were investigated in vitro. The expression of oxidative stress and angiogenesis markers were evaluated on a molecular and protein level using microarray data, immunofluorescence microscopy, Western blot and flow cytometry.

    Results Persistently CDV-infected DH82 cells displayed an increased oxidative stress due to viral infection that leads to a dysregulated HIF-1α expression with a consecutive downregulation of the downstream angiogenetic pathway.

    Conclusion These results indicate that a persistent CDV-infection seems to affect the HIF-1α pathway resulting in a decrease in the downstream production of angiogenetic factors in vitro.


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    Artikel online veröffentlicht:
    08. Juli 2020

    © Georg Thieme Verlag KG
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