CC BY-NC-ND 4.0 · Laryngorhinootologie 2021; 100(S 02): S127
DOI: 10.1055/s-0041-1727977
Abstracts
Head-Neck-Oncology: HPV / Tumor Marker

CD44v3 as a biomarker of tumor derived exosomes in HNSCC patient’s plasma

M-N Theodoraki
1   Uniklinik Ulm, HNO, Ulm
,
P Schuler
1   Uniklinik Ulm, HNO, Ulm
,
S Laban
1   Uniklinik Ulm, HNO, Ulm
,
C Brunner
1   Uniklinik Ulm, HNO, Ulm
,
TK. Hoffmann
1   Uniklinik Ulm, HNO, Ulm
,
TL. Whiteside
2   University of Pittsburgh, Department of Pathology, Pittsburgh, United States
› Author Affiliations
› Further Information
Also available at
 
 

    Background Circulating exosomes play a key role in immune suppression and disease progression and reflect the cargo of their cell origin. By isolating CD3(-) and CD3(+) exosomes from patient’s’ plasma, the cellular origins of immunoregulatory proteins they carry were identified. However, a tumor-specific marker for separation of tumor-derived exosomes (TEX) is still needed. Here we present a method to capture TEX from HNSCC patient’s plasma and to use these exosomes as potential biomarkers for disease stage and metastasis.

    Methods Exosomes were isolated from the plasma of 25 HNSCC patients and 7 healthy donors. Exosomes were separated by immunoaffinity capture using either CD3 or CD44v3. On-bead flow cytometry was used for detection of FAS-L, CSGP4, PD-L1, TGF? or EGFr on exosomes. Results were correlated to clinicopathological parameters.

    Results CD44v3 levels were significantly higher on TEX from HNSCC cell lines as well as in the CD3(-) fraction (enriched in TEX) of plasma derived exosomes. CD44v3 on CD3(-) exosomes correlated significantly with UICC stage and lymph node metastasis. Even more, levels of CD44v3 on CD3(-) exosomes were higher in patients than in healthy donors. Separating exosomes via CD44v3 and staining them for various inhibitory markers showed that CD44v3(+) exosomes had significantly higher levels of PD-L1, CSGP4, TGF?, EGFr and FAS-L compared to CD44v3(-) exosomes. Even more, levels of these markers on CD44v3(+) exosomes correlated with clinicopathological parameters.

    Conclusions Separation of exosomes via CD44v3 presents a way of TEX enrichment and may serve as a liquid biomarker for tumor load and disease stage

    Poster-PDF A-1101.pdf


    #

    Conflict of interest

    Der Erstautor gibt keinen Interessenskonflikt an.

    Address for correspondence

    PD Dr. med. Theodoraki Marie-Nicole
    Uniklinik Ulm, HNO
    Ulm

    Publication History

    Article published online:
    13 May 2021

    © 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

    Georg Thieme Verlag KG
    Rüdigerstraße 14, 70469 Stuttgart, Germany