CC BY-NC-ND 4.0 · Laryngorhinootologie 2021; 100(S 02): S278
DOI: 10.1055/s-0041-1728625
Abstracts
Rhinology: Nasal cavity / Paranasal Sinuses

Effects of gelatine and hyaluronic acid based gels on cell proliferation and migration in an standardised wound model of respiratory epithelia cells

AG Beule
1   HNO Uniklinik Münster, Münster
,
T Voermans
2   Biomed Elements, Nijmegen, Netherlands
,
M Garcia
2   Biomed Elements, Nijmegen, Netherlands
,
K Stübke
1   HNO Uniklinik Münster, Münster
,
C Rudack
1   HNO Uniklinik Münster, Münster
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    Content

    Introduction Otorhinolaryngoloci surgery often results in wounds left for healing in secondary intention. To shorten the postoperative recovery, and decrease the risk of recurrent disease, improvement of wound healing is mandatory. Gelatine and hyaluronic acid are compounds have been incorporated in Epi-Stop Nasal Gel. The aim of this study was to evaluate Epi-Stop compounds separately as two new manufactured formulas and their effect on wound healing in vitro.

    Methods Human nasal epithelial cell were exposed to a gelatine based gel compound (A) and a hyaluronic acid based gel compound (B) in both roti test (for toxicity testing) and a standardized wound model. Residual wound area was measured using digital photographs at 0, 24, 48 and 72h after injury. Medium was analysed for changes in cytokine expression using a multiplex assay in comparison to untreated control and for different dosages (10, 100 and 1000 µg/ml) of A and B. Ciliary beat frequency was measured to exclude a negative effect on mucociliary clearance.

    Results Both gels showed good tissue tolerability. A showed similar wound healing velocity to control. B resulted in accelerated wound healing with a most pronounced effect at 10 µg/ml in a dose-dependant manner. Corresponding to this change, we observed an increased expression of IL-6 (p <  0.03). Ciliary beat frequency remained unchanged for A, but decreased as expected in B due to its high viscosity.

    Discussion Both, compounds seem promising for the postoperative setting, supporting the use in Epi-Stop Nasal Gel final formulation. Increase of wound healing velocity seems to be mediated by increased IL-6 expression in nasal epithelial cells. This finding helps to define molecular targets to improve the postoperative care.

    Poster-PDF A-1255.pdf

    Sponsored by the European Union Interreg Project No. 203020 / 2019P02


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    Conflict of interest

    Der Erstautor gibt keinen Interessenskonflikt an.

    Address for correspondence

    PD Dr. med. Beule Achim Georg
    HNO Uniklinik Münster
    Münster

    Publication History

    Article published online:
    13 May 2021

    © 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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