Exp Clin Endocrinol Diabetes 2017; 125(02): 130-135
DOI: 10.1055/s-0042-116314
Article
© Georg Thieme Verlag KG Stuttgart · New York

Neutral Ceramidase Secreted Via Exosome Protects Against Palmitate-Induced Apoptosis in INS-1 Cells

S. Tang*
1   Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China
,
F. Luo*
1   Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China
,
Y. M. Feng*
1   Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China
,
X. Wei
2   Endocrine and Diabetes Center, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing University of Traditional Chinese Medicine, Branch of China Academy of Chinese Medical Science, Nanjing, China
,
H. Miao
1   Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China
,
Y. B. Lu
1   Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China
,
Y. Tang
1   Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China
,
D. F. Ding
1   Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China
,
J. F. Jin
3   China-USA Lipids in Health and Disease Research Center, Guilin Medical University, Guilin, China
,
Q. Zhu
1   Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China
4   Department of Emergency, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China
› Author Affiliations
Further Information

Publication History

received 16 June 2016
first decision 04 August 2016

accepted 31 August 2016

Publication Date:
22 December 2016 (online)

Abstract

Aims: To investigate the effects of neutral ceramidase (NCDase) packaged in exosomes that are secreted from β-cells on free fatty acid (FFA)-induced β-cells apoptosis and its role in regulation of sphingolipid-mediated signaling pathway.

Methods: HPLC and Western blotting were performed to determine NCDase activity and expression. Annexin V-fluorescein-isothiocyanate/propidium iodide flow cytometry was used to assess apoptosis. Electrospray ionization tandem mass spectrometry was used for ceramide (Cer), sphingosine-1-phosphate (S1P), and sphingosine (SPH) determination.

Results: INS-1 cells over-expressed NCDase secreted active NCDase via exosomes. Exosomes isolated from the cultured medium of INS-1 cells that oxpressed NCDase could ameliorate palmitate-induced apoptosis. Furthermore, the results showed that exosome-derived NCDase treatment reduced intracellular Cer/S1P ratio.

Conclusions: β-cell secreted active NCDase via exosome, the exosome-packaged-NCDase protected β-cells from FFA-induced apoptosis through regulating sphingolipid metabolites and it might be a potential treatment for β-cell lipotoxicity and type 2 diabetes.

* These authors contribute equally to this work.


 
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