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Int J Sports Med 2017; 38(07): 551-559
DOI: 10.1055/s-0042-117723
Immunology
© Georg Thieme Verlag KG Stuttgart · New York

Acute Hematological and Inflammatory Responses to High-intensity Exercise Tests: Impact of Duration and Mode of Exercise

Luciele G. Minuzzi
1   Faculty of Sport Sciences and Physical Education, Coimbra University, Coimbra, Portugal
,
Humberto M. Carvalho
1   Faculty of Sport Sciences and Physical Education, Coimbra University, Coimbra, Portugal
,
Diego T. Brunelli
2   State University of Campinas – UNICAMP, Physical Education, Campinas, Brazil
,
Fatima Rosado
3   CIDAF, Research Centre for Sport and Physical Activity
,
Cláudia R. Cavaglieri
2   State University of Campinas – UNICAMP, Physical Education, Campinas, Brazil
,
Carlos E. Gonçalves
1   Faculty of Sport Sciences and Physical Education, Coimbra University, Coimbra, Portugal
,
Joana M. Gaspar
4   Laboratory of Cell Signaling, Universidade Estadual de Campinas, Campinas, Brazil
,
Luís M. Rama
1   Faculty of Sport Sciences and Physical Education, Coimbra University, Coimbra, Portugal
,
Ana M. Teixeira
5   Faculdade de Ciências do desporto e Educação Física, Centro de Estudos Biocinéticos, Coimbra, Portugal
› Author Affiliations
Further Information

Publication History



accepted after revision 15 September 2016

Publication Date:
08 May 2017 (online)

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Abstract

The purpose of this study was to investigate the hematological and inflammatory responses to 4 maximal high-intensity protocols, considering energy expenditure in each test. 9 healthy volunteers performed 4 high-intensity exercise tests of short [Wingate (WANT); Repeated-sprints (RSA)] and long durations [Continuous VO2 test (VCONT); intermittent VO2 test (VINT)] in a cycle-ergometer, until exhaustion. Hematological parameters and IL-6, IL-10 and creatine kinase (CK) levels were determined before (PRE), POST, 30 min, 1, 2, 12 and 24 h after the end of the protocols. Additionally, energy expenditure was determined. Leucocytes, erythrocytes and lymphocytes increased at POST and returned to PRE values at 30 min for all protocols. Lymphocytes had a second decreased at 2 h and granulocytes increased at 2 h when compared to PRE. Both variables returned to PRE values between 12–24 h into recovery. The magnitude of response for IL-6 was greater in VINT and for IL-10 in VCONT. There was no association of energy expenditure within each exercise protocol with the pattern of IL-6, IL-10 and CK responses to the exercise protocols. The present finding support that similar responses after continuous or intermittent acute protocols are observed when exercises are performed to volitional failure, regardless of the duration and mode of exercise.

Key words

high-intensity exercise - energy expenditure - interleukin-6 - interleukin-10 - recovery
 

  • References

  • 1 Balsom PD, Seger JY, Sjödin B, Ekblom B. Physiological responses to maximal intensity intermittent exercise. Eur J Appl Physiol 1992; 65: 144-149
    CrossrefPubMedGoogle Scholar
  • 2 Balsom PD, Seger JY, Sjödin B, Ekblom B. Maximal-intensity intermittent exercise: effect of recovery duration. Int J Sports Med 1992; 13: 528-533
    Article in Thieme ConnectPubMedGoogle Scholar
  • 3 Banfi G, Del Fabbro M, Mauri C, Corsi MM, Melegati G. Haematological parameters in elite rugby players during a competitive season. Clin Lab Haematol 2006; 28: 183-188
    CrossrefPubMedGoogle Scholar
  • 4 Banfi G, Roi GS, Dolci A, Susta D. Behaviour of haematological parameters in athletes performing marathons and ultramarathons in altitude ('skyrunners'). Clin Lab Haematol 2004; 26: 373-377
    CrossrefPubMedGoogle Scholar
  • 5 Bentley DJ, Newell J, Bishop D. Incremental exercise test design and analysis: Implications for performance diagnostics in endurance athletes. Sport Med 2007; 37: 575-586
    CrossrefPubMedGoogle Scholar
  • 6 Bertuzzi R, Melegati J, Bueno S, Ghiarone T, Pasqua LA, Gáspari AF, Lima-Silva AE, Goldman A. GEDAE-LaB: A Free Software to Calculate the Energy System Contributions during Exercise. PLoS One 2016; 11: e0145733
    CrossrefPubMedGoogle Scholar
  • 7 Caton AJ, Weissler KA. Regulatory cells in health and disease. Immunol Rev 2014; 259: 5-10
    CrossrefPubMedGoogle Scholar
  • 8 Cipryan L, Svagera Z, Vala R. IL-6 and CRP response to maximal exercise intervention. J Sports Med Phys Fitness 55: 813-823
    PubMedGoogle Scholar
  • 9 Dawson B, Goodman C, Lawrence S, Preen D, Polglaze T, Fitzsimons M, Fournier P. Muscle phosphocreatine repletion following single and repeated short sprint efforts. Scand J Med Sci Sports 1997; 7: 206-213
    PubMedGoogle Scholar
  • 10 Dill DB, Costill DL. Calculation of percentage changes in volumes of blood, plasma and red cells in dehydration. J Appl Physiol 1974; 37: 247-248
    CrossrefPubMedGoogle Scholar
  • 11 Friedman RA, Navalta JW, Fedor EA, Kell HB, Lyons TS, Arnett SW, Schafer MA. Repeated high-intensity Wingate cycle bouts influence markers of lymphocyte migration but not apoptosis. Appl Physiol Nutr Metab 2012; 37: 241-246
    CrossrefPubMedGoogle Scholar
  • 12 Gleeson M. Immune function in sport and exercise. J Appl Physiol 2007; 103: 693-699
    CrossrefPubMedGoogle Scholar
  • 13 Gleeson M, Bishop N, Oliveira M, McCauley T, Tauler P, Muhamad AS. Respiratory infection risk in athletes: association with antigen-stimulated IL-10 production and salivary IgA secretion. Scand J Med Sci Sport 2012; 22: 410-417
    CrossrefPubMedGoogle Scholar
  • 14 Gleeson M, Bishop NC, Stensel DJ, Lindley MR, Mastana SS, Nimmo MA. The anti-inflammatory effects of exercise: mechanisms and implications for the prevention and treatment of disease. Nat Rev Immunol 2011; 11: 607-615
    CrossrefPubMedGoogle Scholar
  • 15 Gleeson M, Walsh NP. The BASES expert statement on exercise, immunity, and infection. J Sports Sci 2012; 30: 321-324
    CrossrefPubMedGoogle Scholar
  • 16 Handzlik MK, Shaw AJ, Dungey M, Bishop NC, Gleeson M, Handzlik MK, Shaw AJ, Dungey M, Bishop NC, Gleeson M. The influence of exercise training status on antigen-stimulated IL-10 production in whole blood culture and numbers of circulating regulatory T cells. Eur J Appl Physiol 2013; 113: 1839-1848
    CrossrefPubMedGoogle Scholar
  • 17 Harriss DJ, Atkinson G. Ethical Standards in Sport and Exercise Science Research: 2016 Update. Int J Sports Med 2015; 36: 1121-1124
    Article in Thieme ConnectPubMedGoogle Scholar
  • 18 Henriksson J, Sahlin K. Metabolism during exercise – energy expenditure and hormonal changes. In Textbook of sports medicine. In: Kjaer M, Krogsgaard M, Magnusson P, Engebretsen L, Roos H, Takala TWS-Y. (ed.). Malden, Massachusetts: Blackwell Science Ltd; 2003
    Google Scholar
  • 19 Hovanloo F, Arefirad T, Ahmadizad S. Effects of sprint interval and continuous endurance training on serum levels of inflammatory biomarkers. J Diabetes Metab Disord 2013; 12: 22
    CrossrefPubMedGoogle Scholar
  • 20 LaVoy EC, Bosch JA, Lowder TW, Simpson RJ, Emily L. Acute aerobic exercise in humans increases cytokine expression in {CD27(-)} but not {CD27(+)} {CD8(+)} T-cells. Brain Behav Immun 2013; 27: 54-62
    CrossrefPubMedGoogle Scholar
  • 21 Margarida S, Anne O, Saraiva M, O’Garra A. The regulation of {IL-10} production by immune cells. Nat Rev Immunol 2010; 10: 170-181
    CrossrefPubMedGoogle Scholar
  • 22 McCarthy DA, Macdonald I, Grant M, Marbut M, Watling M, Nicholson S, Deeks JJ, Wade AJ, Perry JD. Studies on the immediate and delayed leucocytosis elicited by brief (30-min) strenuous exercise. Eur J Appl Physiol 1992; 64: 513-517
    CrossrefPubMedGoogle Scholar
  • 23 Navalta JW, McFarlin BK, Lyons TS. Does exercise really induce lymphocyte apoptosis?. Front Biosci (Elite Ed) 2010; 2: 478-488
    PubMedGoogle Scholar
  • 24 Navalta JW, Tibana RA, Fedor EA, Vieira A, Prestes J. Three consecutive days of interval runs to exhaustion affects lymphocyte subset apoptosis and migration. Biomed Res Int 2014; 2014: 694801
    PubMedGoogle Scholar
  • 25 Nguyen T, Obeid J, Ploeger HE, Takken T, Pedder L, Timmons BW. Inflammatory and growth factor response to continuous and intermittent exercise in youth with cystic fibrosis. J Cyst Fibros 2012; 11: 108-118
    CrossrefPubMedGoogle Scholar
  • 26 Nieman D, Henson D, Gojanovich G, Davis JM, Dumke C, Utter A, Murphy A, Pearce S, McAnulty S, McAnulty L. Immune changes: 2h of continuous vs. intermittent cycling. Int J Sports Med 2007; 28: 625-630
    Article in Thieme ConnectPubMedGoogle Scholar
  • 27 Nieman DC. Immune response to heavy exertion. J Appl Physiol 1997; 82: 1385-1394
    CrossrefPubMedGoogle Scholar
  • 28 Nieman DC, Zwetsloot KA, Meaney MP, Lomiwes DD, Hurst SM, Hurst RD. Post-Exercise Skeletal Muscle Glycogen Related to Plasma Cytokines and Muscle IL-6 Protein Content, but not Muscle Cytokine mRNA Expression. Front Nutr 2015; 2:
    PubMed
  • 29 Northoff H, Berg A. Immunologic mediators as parameters of the reaction to strenuous exercise. Int J Sports Med 1991; 12 (Suppl. 01) S9-S15
    Article in Thieme ConnectPubMedGoogle Scholar
  • 30 Nunes LAS, Grotto HZW, Brenzikofer R, Macedo DV. Hematological and biochemical markers of iron status in a male, young, physically active population. Biomed Res Int 2014; 2014: 349182
    PubMedGoogle Scholar
  • 31 Ostrowski K, Schjerling P, Pedersen BK. Physical activity and plasma interleukin-6 in humans – effect of intensity of exercise. Eur J Appl Physiol 2000; 83: 512-515
    CrossrefPubMedGoogle Scholar
  • 32 Peake JM. Exercise-induced alterations in neutrophil degranulation and respiratory burst activity: Possible mechanisms of action. Exerc Immunol Rev 2002; 8: 49-100
    PubMedGoogle Scholar
  • 33 Peake JM, Della Gatta P, Suzuki K, Nieman DC. Cytokine expression and secretion by skeletal muscle cells: regulatory mechanisms and exercise effects. Exerc Immunol Rev 2015; 21: 8-25
    Google Scholar
  • 34 Pedersen B, Hoffman-Goetz L. Exercise and the immune system: regulation, integration, and adaptation. Physiol Rev 2000; 80: 1055-1081
    CrossrefPubMedGoogle Scholar
  • 35 Pedersen BK, Febbraio MA. Muscles, exercise and obesity: skeletal muscle as a secretory organ. Nat Rev Endocrinol 2012; 8: 457-465
    CrossrefPubMedGoogle Scholar
  • 36 Pedersen BK, Fischer CP. Beneficial health effects of exercise – the role of IL-6 as a myokine. Trends Pharmacol Sci 2007; 28: 152-156
    CrossrefPubMedGoogle Scholar
  • 37 Petersen A, Pedersen B. The anti-inflammatory effect of exercise. J Appl Physiol 2005; 98: 1154-1162
    CrossrefPubMedGoogle Scholar
  • 38 Reihmane D, Dela F. Interleukin-6: possible biological roles during exercise. Eur J Sport Sci 2014; 14: 242-250
    CrossrefPubMedGoogle Scholar
  • 39 Reihmane D, Jurka A, Tretjakovs P, Dela F. Increase in IL-6, TNF-α, and MMP-9, but not sICAM-1, concentrations depends on exercise duration. Eur J Appl Physiol 2013; 113: 851-858
    CrossrefPubMedGoogle Scholar
  • 40 Sari-Sarraf V, Reilly T, Doran DA. Salivary IgA response to intermittent and continuous exercise. Int J Sports Med 2006; 27: 849-855
    Article in Thieme ConnectPubMedGoogle Scholar
  • 41 Sim M, Dawson B, Landers G, Swinkels DW, Tjalsma H, Trinder D, Peeling P. Effect of exercise modality and intensity on post-exercise interleukin-6 and hepcidin levels. Int J Sport Nutr Exerc Metab 2013; 23: 178-186
    CrossrefPubMedGoogle Scholar
  • 42 Simpson RJ, Florida-James GD, Cosgrove C, Whyte GP, Macrae S, Pircher H, Guy K. High-intensity exercise elicits the mobilization of senescent T lymphocytes into the peripheral blood compartment in human subjects. J Appl Physiol 2007; 103: 396-401
    CrossrefPubMedGoogle Scholar
  • 43 Simpson RJ, Kunz H, Agha N, Graff R. Exercise and the Regulation of Immune Functions. Prog Mol Biol Transl Sci 2015; 135: 355-380
    Google Scholar
  • 44 Speaker KJ, Cox SS, Paton MM, Serebrakian A, Maslanik T, Greenwood BN, Fleshner M. Six weeks of voluntary wheel running modulates inflammatory protein (MCP-1, IL-6, and IL-10) and DAMP (Hsp72) responses to acute stress in white adipose tissue of lean rats. Brain Behav Immun 2014; 39: 87-98
    CrossrefPubMedGoogle Scholar
  • 45 Spencer M, Bishop D, Dawson B, Goodman C. Physiological and metabolic responses of repeated-sprint activities: specific to field-based team sports. Sports Med 2005; 35: 1025-1044
    CrossrefPubMedGoogle Scholar
  • 46 Suzuki K, Nakaji S, Yamada M, Liu Q, Kurakake S, Okamura N, Kumae T, Umeda T, Sugawara K. Impact of a competitive marathon race on systemic cytokine and neutrophil responses. Med Sci Sports Exerc 2003; 35: 348-355
    CrossrefPubMedGoogle Scholar
  • 47 Tidball JG, Villalta SA. Regulatory interactions between muscle and the immune system during muscle regeneration. Am J Physiol 2010; 298: R1173-R1187
    PubMedGoogle Scholar
  • 48 Tossige-Gomes R, Ottone VO, Oliveira PN, Viana DJS, Araujo TL, Gripp FJ, Rocha-Vieira E. Leukocytosis, muscle damage and increased lymphocyte proliferative response after an adventure sprint race. Braz J Med Biol Res 2014; 47: 492-498
    CrossrefPubMedGoogle Scholar
  • 49 Walsh N, Gleeson MM, Shephard R, Woods J, Bishop N, Fleshner M, Green C, Pedersen B, Laurie H-G, Rogers C, Northoff H, Abbasi A, Simon P. Position statement. Part one: Immune function and exercise. Exerc Immunol Rev 2011; 17: 6-63
    PubMedGoogle Scholar
  • 50 Weng T-P, Huang S-C, Chuang Y-F, Wang J-S. Effects of interval and continuous exercise training on CD4 lymphocyte apoptotic and autophagic responses to hypoxic stress in sedentary men. PLoS One 2013; 8: e80248
    CrossrefPubMedGoogle Scholar
  • 51 Zwetsloot KA, John Casey S, Lawrence MM, Battista RA, Shanely RA, Andrew Shanely R. High-intensity interval training induces a modest systemic inflammatory response in active, young men. J Inflamm Res 2014; 7: 9-17
    PubMedGoogle Scholar