Laryngorhinootologie 2023; 102(S 02): S213
DOI: 10.1055/s-0043-1767142
Abstracts | DGHNOKHC
Infectiology/Hygiene: Covid-19

Spike-beads as a model to investigate the effect of immune complexes on platelet aggregation in Covid-19

Julie Petry
1   Klinikum rechts der Isar der TUM, Klinik und Polyklinik für Hals-, Nasen- und Ohrenheilkunde
,
Lena Griesbaum
1   Klinikum rechts der Isar der TUM, Klinik und Polyklinik für Hals-, Nasen- und Ohrenheilkunde
,
Admar Verschoor
1   Klinikum rechts der Isar der TUM, Klinik und Polyklinik für Hals-, Nasen- und Ohrenheilkunde
,
Barbara Wollenberg
1   Klinikum rechts der Isar der TUM, Klinik und Polyklinik für Hals-, Nasen- und Ohrenheilkunde
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    Prothrombotic events are prominent hallmarks of a severe Covid-19 infection. Although the exact mechanisms underlying these thromboinflammations are still unclear, evidence suggests that platelets play a crucial role. Studies have shown that platelets from Covid-19 patients are hyperactivated, leading to the formation of microthrombi and providing a higher risk of mortality. Hence, we investigated the impact of spike-coated beads on platelets to find a major mechanism, leading to platelet hyperactivation. Activation of platelets was investigated after coincubation of washed platelets from healthy donors and spike-coated beads. Aggregation and activation were measured by the use of aggregometry and FACS staining of activation markers. Stimulation of platelets with spike-beads induced platelet aggregation and expression of activation markers. This activation process required the presence of plasma, pointing to the contribution of soluble factors. Considering that immune complexes are able to activate platelets, we assessed their contribution to spike-beads-induced platelet activation. Indeed, spike-beads can bind IgG in plasma from vaccinated donors. Using a blocking antibody for the FcγRIIa receptor, we were able to inhibit platelet aggregation by spike-beads, suggesting that the activation occurs through stimulation of the low-affinity receptor for IgG. Taking into account, that prothrombotic events are induced by multiple factors, it is important to elucidate this process in detail to understand the pathobiology. Here, we describe a powerful model, which is useful to dissect multifactorial-induced thrombotic events. Overall, this study provides a possible mechanism of platelet activation in a SARS-CoV-2 infection, which opens new avenues for therapeutic options.


     

    Conflict of Interest

    The authors declare that they have no conflict of interest.

    Publikationsverlauf

    Artikel online veröffentlicht:
    12. Mai 2023

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