Structural Adaptations of the Anterolateral Complex of the Knee and Associated Tissues: A Comparative Anatomical and Histological Analysis of Knees With and Without an Anterolateral Ligament

Charles R. Marchese; Benjamin Pautler; Makayla M. Swancutt; Bryan G. Beutel

doi:10.1055/s-0044-1801749

The Journal of Knee Surgery, Inhaltsverzeichnis

J Knee Surg 2025; 38(05): 237-244
DOI: 10.1055/s-0044-1801749

Original Article

Structural Adaptations of the Anterolateral Complex of the Knee and Associated Tissues: A Comparative Anatomical and Histological Analysis of Knees With and Without an Anterolateral Ligament

Charles R. Marchese

¹Department of Pathology and Anatomical Sciences, Kansas City University College of Medicine, Kansas City, Missouri

,

Benjamin Pautler

¹Department of Pathology and Anatomical Sciences, Kansas City University College of Medicine, Kansas City, Missouri

,

Makayla M. Swancutt

¹Department of Pathology and Anatomical Sciences, Kansas City University College of Medicine, Kansas City, Missouri

,

Bryan G. Beutel

²Department of Primary Care, Kansas City University College of Medicine, Kansas City, Missouri

› Institutsangaben

Abstract

The anterolateral ligament (ALL) is considered a secondary stabilizer of internal rotation and, due to proximity to the lateral collateral ligament (LCL), it may contribute to anterolateral rotatory stability. This study characterized the anatomy of the ALL and associated tissues of the anterolateral complex (ALC) to determine if structural and histological compensatory adaptations exist in patients without an ALL. Forty-nine cadaveric knees were dissected from distal-to-proximal using established landmarks with the aid of internal rotation stress to localize the ALL (if present), LCL, iliotibial band (ITB), and anterolateral capsule. The width and thickness of ALL and LCL were measured with digital calipers at the origin, middle, and insertion, and cross-sectional areas were calculated. ALL and LCL length and ITB thickness were also obtained. Samples of each tissue were stained with hematoxylin and eosin and picrosirius red, and histological images were evaluated with ImageJ to quantify collagen density (mean gray value [mgv]) and quantity (percent coverage). Size measurements and collagen characteristics were compared between ALL-present and ALL-deficient knees. The ALL was identified in 63% of knees with mean cross-sectional areas of 8.9, 5.8, and 9.7 mm² at the origin, middle, and insertion, respectively. Mean collagen density of the ALL was 106.9 mgv on a scale of 0 (black) to 255 (white), and overall collagen quantity was 40.3%. Proximal LCL width (p = 0.04), distal LCL thickness (p = 0.03), and cross-sectional area (p = 0.01), and ITB thickness (p = 0.02) were significantly greater in ALL-deficient knees. A significantly higher collagen density was found within the LCL (p = 0.04), and higher overall quantity of collagen within the LCL (p < 0.01) and ITB (p < 0.01), of ALL-deficient knees. Gross anatomical and histological alterations exist in knees without an ALL compared with those with an ALL. These may reflect adaptations in the ALC and LCL that are present to compensate for the absence of the anterolateral rotatory stability afforded by the ALL.

Keywords

anterolateral ligament - anterolateral complex - anatomy - histology - adaptation

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Referenzen

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