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DOI: 10.1055/s-0045-1807776
Increased ager expression in inflammatory breast cancer: a comparative study with non-ibc patients
Authors
Introduction: Inflammatory breast cancer (IBC) is a rare and aggressive form of breast cancer characterized by erythema, edema, and skin changes such as nipple inversion and peau d'orange. These symptoms are primarily due to the presence of numerous dermal tumor emboli that obstruct lymphatic drainage. Despite the distinct symptomatology between IBC and non-IBC, the molecular differences between these two forms of breast cancer remain largely unexplored. The Advanced Glycation End Product Receptor (AGER/RAGE) is a transmembrane receptor overexpressed in various cancers, is associated with epithelial-to-mesenchymal transition, and has been implicated in breast cancer pathogenesis. The overexpression of AGER is linked to rapid tumor progression and metastasis.
Objective: To measure and compare the levels of AGER expression in patients with IBC and non-IBC.
Method: Indirect immunofluorescence was analyzed in 35 samples (19 IBC and 16 non-IBC). Both sample types were stained with DAPI, which binds to the nuclear area, and an anti-AGER monoclonal antibody, which binds to the receptor. Four fields were selected for each sample, and AGER expression was quantified as a percentage of the immunofluorescence intensity area. The expression data and clinicopathological parameters were compared, including the prognostic values of these biomarkers. The transcriptomic data of IBC and non-IBC microarray data from the Gene Expression Omnibus repository (GEO) were used to investigate the expression of AGER genes.
Results: Tumor samples from IBC patients showed higher AGER immunoexpressions than the non-IBC group and were associated with obesity and Ki-67 expression (p <0.05). AGER expression in IBC versus non-IBC was also statistically associated with triple-negative molecular subtypes. External validation by analyzing three external GEO datasets confirmed the higher expression of AGER in IBC than in non-IBC samples.
Conclusion: Overall, tumor samples from IBC patients showed higher AGER expressions than other breast cancer types.
Corresponding author: Pedro Ruan Amorim de Oliveira Costa (e-mail: pedroruan@alu.ufc.br).
Die Autoren geben an, dass kein Interessenkonflikt besteht.
Publikationsverlauf
Artikel online veröffentlicht:
06. Mai 2025
© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/)
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Pedro Ruan Amorim de Oliveira Costa, Bianca Elen de Souza Alves, Mariana Timbaúba Benício Coelho, Jeferson dos Santos Souza, Ana Carolina Filgueiras Teles, Luiza Darla Aguiar Silva Paiva, Isabelle de Fátima Vieira Camelo Maia, Jailson de Sousa Oliveira, Maria do Perpétuo Socorro Saldanha da Cunha, Paulo Roberto Carvalho de Almeida, Larissa Mont'Alverne de Arruda, Silvia Regina Rogatto, Roberto César Pereira Lima-Júnior, Deysi Viviana Tenazoa Wong. Increased ager expression in inflammatory breast cancer: a comparative study with non-ibc patients. Brazilian Journal of Oncology 2025; 21.
DOI: 10.1055/s-0045-1807776