Phenolic and Oxidative Metabolism as Bioindicators of Nitrogen Deficiency in French Bean Plants (Phaseolus vulgaris L. cv. Strike)

E. Sánchez; J. M. Soto; P. C. García; L. R. López-Lefebre; R. M. Rivero; J. M. Ruiz; L. Romero

doi:10.1055/s-2000-3699

Plant Biology, Inhaltsverzeichnis

Plant Biol (Stuttg) 2000; 2(3): 272-277
DOI: 10.1055/s-2000-3699

Original Paper

Georg Thieme Verlag Stuttgart ·New York

Phenolic and Oxidative Metabolism as Bioindicators of Nitrogen Deficiency in French Bean Plants (Phaseolus vulgaris L. cv. Strike)

E. Sánchez ¹ , J. M. Soto ² , P. C. García ¹ , L. R. López-Lefebre ¹ , R. M. Rivero ¹ , J. M. Ruiz ¹ , L. Romero ¹

¹ Department of Plant Biology, Faculty of Sciences, University of Granada, Granada, Spain
² Department of Agrotechnology Science, University of Chihuahua, Chihuahua, México

Abstract

The aim of the present work was to determine the effect of abiotic stress, such as nitrogen (N) deficiency, on phenol and oxidative metabolism. In addition, we analyzed whether the response of the two metabolic processes is a good bioindicator of N deficiency in French bean plants. The N was applied to the nutrient solution in the form of NH₄NO₃ at 1.35 mM (N1), 2.7 mM (N2) and 5.4 mM (N3), this latter dosage being considered optimal. The results indicated that application of 1.35 and 2.70 mM of N can be defined as suboptimal or deficient, as it depressed foliar biomass of the French bean plants in our experiment. In addition, abiotic stress from the application of these N dosages stimulated the enzymes PPO, POD and CAT, and inhibited PAL and SOD activities, resulting in the lowest foliar accumulation of phenolic compounds and H₂O₂.

Abbreviations

AOS: active oxygen species

CAT: catalase

DW: dry weight

EDDHA: ethylenediamine-di(o-hydroxyphenylacetic acid)

FW: fresh weight

PAL: phenylalanine ammonia-lyase

POD: peroxidase

PPO: polyphenol oxidase

SOD: superoxide dismutase

Key words

Phaseolus vulgaris - French bean - nitrogen - phenolic compounds - oxidative metabolism

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E. Sánchez

Department of Plant Biology Faculty of Sciences

University of Granada 18071 Granada Spain

eMail: lromero@goliat.ugr.es

Section Editor: U. Lüttge