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ZWR - Das Deutsche Zahnärzteblatt 2003; 112(7/08): 311-318
DOI: 10.1055/s-2003-41757
Originalia
Chirurgie
© Georg Thieme Verlag Stuttgart · New York

Wirkung von Knochenersatzmaterialien auf die Osteoblastenproliferation

Effectiveness of Bone Graft Materials on the Proliferation of OsteoblastsJ. Neugebauer1 , J. -H. Oh2 , M. Scheer1 , A. C. Kübler1
  • 1Klinik und Poliklinik für Zahnärztliche Chirurgie und Mund-, Kiefer- und Plastische Gesichtschirurgie, Universität zu Köln (Direktor: Univ.-Prof. Dr. Dr. J. E. Zöller)
  • 2Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyung-Hee University, Republic of Korea
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Publication History

Publication Date:
01 September 2003 (online)

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Zusammenfassung

Im Rahmen einer In-vitro-Studie an humanen Osteoblasten wurde der Einfluss verschiedener Knochenersatzmaterialien auf das Proliferationsverhalten und die Differenzierungsfähigkeit WST-2-Test und der Bestimmung der alkalischen Phosphatase untersucht. Der Vergleich der eingesetzten Materialien ergab für PepGen P-15™ die höchsten Proliferations- und Differenzierungsrate, gefolgt von Osteograf®/N, Algipore® und BioBase®. Bei BioOss® wurden die niedrigsten Werte gemessen. Mittels der vorgestellten Methodik scheint ein standardisierter Vergleich der osteokonduktiven Knochenersatzmaterialien im In-vitro-Modell möglich.

Summary

The effect of 5 different bone graft materials on the proliferation patterns of human osteoblasts were investigated in-vitro by the WST-1-Test and determination of alkaline phosphatase. The cultures were examined for cell growth behavior and morphology by phase contrast light and scanning electron microscopy. The comparison for the various materials showed the highest proliferation and differentiation rate for PepGen P-15™ followed by Osteograf®/N, Algipore® and BioBase®. BioOss® showed the lowest. The demonstrated study design seems to be beneficial for a standardized comparison of osteoconductive bone graft materials in-vitro.

Key Words

Knochenersatzmaterial - Osteoblasten - Proliferation

Key Words

Bone Graft Materials - Osteoblast - Proliferation

Literatur

  • 1 Acil Y, Springer IN, Broek V, Terheyden H, Jepsen S. Effects of bone morphogenetic protein-7 stimulation on osteoblasts cultured on different biomaterials.  J Cell Biochem. 2002;  86 90-98
    CrossrefPubMedGoogle Scholar
  • 2 Al KA Ruhaimi. Bone graft substitutes: A comparative qualitative histologic review of current osteoconductive grafting materials.  Int J Oral Maxillofac Implants. 2001;  16 105-114
    PubMedGoogle Scholar
  • 3 Bhatnagar RS, Qian JJ, Wedrychowska A, Dixon E, Smith N. Biomimetic habitats for cells: Ordered matrix deposition and differentiation in gingival fibroblast cultured on hydroxyapatite coated with a collagen analogue.  Cell and Materials. 1999;  9 93-104
    PubMedGoogle Scholar
  • 4 Buser D, Dula K, Belser U, Hirt HP, Berthold H. Localized ridge augmentation using guided bone regeneration. 1. Surgical procedure in the maxilla.  Int J Periodontics Restorative Dent. 1993;  13 29-45
    PubMedGoogle Scholar
  • 5 Callan DP, Salkeld SL, Scarborough N. Histologic analysis of implant sites after grafting with demineralized bone matrix putty and sheets.  Implant Dent. 2000;  9 36-44
    PubMedGoogle Scholar
  • 6 Davies JE. Mechanisms of endosseous integration.  Int J Prosthodont. 1998;  11 391-401
    PubMedGoogle Scholar
  • 7 Froum SJ, Tarnow DP, Wallace SS, Rohrer MD, Cho SC. Sinus floor elevation using anorganic bovine bone matrix (OsteoGraf®/N) with and without autogenous bone: A clinical, histologic, radiographic, and histomorphometric analysis - Part 2 of an ongoing prospective study.  Int J Periodont Restorative Dent. 1998;  18 528-543
    PubMedGoogle Scholar
  • 8 Fugazzotto PA, Shanaman R, Manos T, Shectman R. Guided bone regeneration around titanium implants: Report of the treatment of 1,503 sites with clinical reentries.  Int J Periodont Restorative Dent. 1997;  17 292-299
    PubMedGoogle Scholar
  • 9 Haas R, Haidvogl D, Dortbudak O, Mailath G. Freeze-dried bone for maxillary sinus augmentation in sheep. Part II: Biomechanical findings.  Clin Oral Implants Res. 2002;  13 581-586
    CrossrefPubMedGoogle Scholar
  • 10 Haessler D, Vizethum F, Zoller JE. Autogene Knochentransplantation mit Hilfe eines Spankollectors - eine Methodenbeschreibung.  Implantologie. 1999;  4 315-322
    PubMedGoogle Scholar
  • 11 Hall EE, Meffert RM, Hermann JS, Mellonig JT, Cochran DL. Comparison of bioactive glass to demineralized freeze-dried bone allograft in the treatment of intrabony defects around implants in the canine mandible.  J Periodontol. 1999;  70 526-535
    CrossrefPubMedGoogle Scholar
  • 12 Hidding J, Lazar F, Zoller JE. Erste Ergebnisse bei der vertikalen Distraktionsosteogenese des atrophischen Alveolarkamms (Initial outcome of vertical distraction osteogenesis of the atrophic alveolar ridge).  Mund Kiefer Gesichtschir. 1999;  3 79-83
    PubMedGoogle Scholar
  • 13 Huffman EWDJ, Keil RL. Determination of trace organic carbon and nitrogen in the presence of carbonates in anorganic bovine bone graft materials.  Microchem J. 2003;  74 249-256
    PubMedGoogle Scholar
  • 14 Hurzeler MB, Quinones CR, Kirsch A. et al. . Maxillary sinus augmentation using different grafting materials and dental implants in monkeys. Part I. Evaluation of anorganic bovine-derived bone matrix.  Clin Oral Implants Res. 1997;  8 476-486
    CrossrefPubMedGoogle Scholar
  • 15 Jakse N, Seibert FJ, Lorenzoni M, Eskici A, Pertl C. A modified technique of harvesting tibial cancellous bone and its use for sinus grafting.  Clin Oral Implants Res. 2001;  12 488-494
    CrossrefPubMedGoogle Scholar
  • 16 Khoury F, Pingel D, Joos U. Die Sinusbodenelevation mit simultaner Implantation unter Verwendung von Knochentransplantaten aus dem Unterkiefer. Erster Erfahrungsbericht.  Z Zahnärztl Implant. 1993;  9 175-180
    PubMedGoogle Scholar
  • 17 Lallier TE, Palaiologou AA, Yukna RA, Layman DL. The putative collagen-binding peptide P-15 promotes fibroblast attachment to root shavings but not hydroxylapatite.  J Periodontol. 2003;  74 458-674
    CrossrefPubMedGoogle Scholar
  • 18 Lorenzoni M, Pertl C, Keil C, Wegscheider WA. Treatment of peri-implant defects with guided bone regeneration: A comparative clinical study with various membranes and bone grafts.  Int J Oral Maxillofac Implants. 1998;  13 639-646
    PubMedGoogle Scholar
  • 19 Lumbikanonda N, Sammons R. Bone cell attachment to dental implants of different surface characteristics.  Int J Oral Maxillofac Implants. 2001;  16 627-636
    PubMedGoogle Scholar
  • 20 MacNeill SR, Cobb CM, Rapley JW, Glaros AG, Spencer P. In vivo comparison of synthetic osseous graft materials. A preliminary study.  J Clin Periodontol. 1999;  26 239-245
    CrossrefPubMedGoogle Scholar
  • 21 Merten HA, Wiltfang J, Grohmann U, Hoenig JF. Intraindividual comparative animal study of alpha- and beta-tricalcium phosphate degradation in conjunction with simultaneous insertion of dental implants.  J Craniofac Surg. 2001;  12 59-68
    PubMedGoogle Scholar
  • 22 Qian JJ, Bhatnagar RS. Enhanced cell attachment to anorganic bone mineral in the presence of a synthetic peptide related to collagen.  J Biomed Mater Res. 1996;  31 545-554
    PubMedGoogle Scholar
  • 23 Roggendorf W, Strunz V, Gross U. Osteoblasten Kulturen. Ein Modell zur Testung von Biomaterialien.  Z Zahnaärztl Implantol. 1986;  2 197-202
    PubMedGoogle Scholar
  • 24 Schopper C, Moser D, Wanschitz F. et al. . Histomorphologic findings on human bone samples six months after bone augmentation of the maxillary sinus with Algipore®.  J Long Term Eff Med Implants. 1999;  9 203-213
    PubMedGoogle Scholar
  • 25 Smiler DG, Johnson PW, Lozada JL. et al. . Sinus lift grafts and endosseous implants. Treatment of the atrophic posterior maxilla.  Dent Clin North Am. 1992;  36 151-186
    PubMedGoogle Scholar
  • 26 Summers BN, Eisenstein SM. Donor site pain from the ilium. A complication of lumbar spine fusion.  J Bone Joint Surg Br. 1989;  71 677-680
    PubMedGoogle Scholar
  • 27 Watzinger F, Luksch J, Millesi W. et al. . Guided bone regeneration with titanium membranes: A clinical study.  In: Br J Oral Maxillofac Surg. 2000;  38 312-315
    PubMedGoogle Scholar
  • 28 Weibrich G, Gotz H, Gnoth SH. et al. . Charakterisierung der Oberflächenstruktur von Knochenersatzmaterialien mittels REM.  Z Zahnärztl Implantol. 2000;  16 151-159
    PubMedGoogle Scholar
  • 29 Wenz B, Oesch B, Horst M. Analysis of the risk of transmitting bovine spongiform encephalopathy through bone grafts derived from bovine bone.  Biomaterials. 2001;  22 1599-1606
    CrossrefPubMedGoogle Scholar
  • 30 Zitzmann NU, Scharer P, Marinello CP, Schupbach P, Berglundh T. Alveolar ridge augmentation with Bio-Oss®: A histologic study in humans.  Int J Periodont Restorative Dent. 2001;  21 288-295
    PubMedGoogle Scholar

Korrespondenzadresse

Prof. Dr. Dr. Alexander C. Kübler

Klinik und Poliklinik für Zahnärztliche Chirurgie und für Mund-, Kiefer- und Plastische Gesichtschirurgie der Universität zu Köln, Direktor: Univ.-Prof. Dr. Dr. J. E. Zöller

Kerpener Str. 32

50931 Köln

Phone: 0221/4785791

Fax: 0221/4787360



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