Licorice Consumption and Serum Testosterone in Healthy Man

Abstract

We have previously found that licorice can reduce serum testosterone in healthy men. These results were not confirmed in another study, where the same amounts of licorice did not decrease salivary testosterone values. In the actual study we treated more cases with the same amount of licorice and reproduced our previous data. The mean testosterone values decreased by 26 % after one week of treatment (p < 0.01). There was also a significant increase in 17-OHP and LH concentrations and a slight, but not significant decrease in free testosterone. Licorice treatment, in addition, did not affect the response of testosterone and 17-OHP to stimulation with β-HCG.

Licorice - glycyrrhetinic acid - total testosterone - free testosterone

References

• 1 Armanini D, Bonanni G, Palermo M. Reduction of serum testosterone in men by licorice.  N Engl J Med. 1999;  341 1158
• 2 Armanini D, Fiore C, Mattarello M J, Bielenberg J, Palermo M. History of the endocrine effects of licorice.  Exp Clin Endocrinol Diabetes. 2002;  110 1-5
• 3 Armanini D, Lewicka S, Pratesi C, Scali M, Zennaro M C, Zovato S, Gottardo C, Simoncini M, Spigariol A, Zampollo V. Further studies on the mechanism of the mineralocorticoid action of licorice in humans.  J Endocrinol Invest. 1996;  19 624-629
• 4 Bernardi M, D'Intino P, Trevisani F, Cantelli-Forti G I, Raggi M A, Turchetto E, Gasbarrini G. Effects of prolonged ingestion of graded doses of licorice by healthy volunteers.  Life Science. 1994;  55 863
• 5 Farese R V, Biglieri E G, Shackleton C HL, Irony I, Gomez-Fontes R. Licorice-induced hypermineralocorticoidism.  New Engl J Med. 1991;  325 1225-1227
• 6 Funder J W, Pearce P T, Smith R, Smith A. Mineralocorticoid action: target tissue specificity is enzyme not receptor mediated.  Science. 1988;  242 583-585
• 7 Ge R S, Gao H B, Nacharaju V L, Gunsalus G L, Hardy M P. Identification of a kinetically distinct activity of 11β- hydroxysteroid dehydrogenase in rat Leydig cells.  Endocrinology. 1996;  138 2435-2442
• 8 Hardy M P, Ganjam V K. Stress, 11 beta-HSD, and Leydig cell function.  J Androl. 1997;  18 475-479
• 9 Josephs R A, Guinn J S, Harper M L, Askari F. Liquorice consumption and salivary testosterone concentrations.  Lancet. 2001;  358 1613-1614
• 10 Leckie C M, Wellberg L A, Seckl J R. 11 beta-hydroxysteroid dehydrogenase is a predominant reductase in intact rat Leydig cells.  J Endocrinol. 1998;  159 233-238
• 11 Monder C, Hardy M P, Blanchard R J, Blanchard D C. Comparative aspects of 11β-hydroxysteroid dehydrogenase. Testicular 11βhydroxysteroid-dehydrogenase. Development of a model for the mediation of Leydig cells function by corticosteroids.  Steroids. 1994;  59 69-73
• 12 Palermo M, Gomez-Sanchez C, Roitman E, Schackleton C HL. Quantitation of cortisol 3-oxo-4-ene steroids in urine using gas chromatography/mass spectrometry with stable isotope-labeled internal standars.  Steroids. 1996;  61 583-589
• 13 Sakamoto K, Wakabayashi K. Inhibitory effect of glycyrrhetinic acid on testosterone production in rat gonads.  Endocrinol Jpn. 1988;  35 333-342
• 14 Stewart P M, Krozowski Z S. 11 Beta-hydroxysteroid dehydrogenase.  Vitam Horm. 1999;  57 249-324
• 15 Takeuchi T, Nishii O, Okamura T, Yaginuma T. Effect of paeoniflorin, glycyrrhizin and glycyrrhetinic acid on ovarian androgen production.  Am J Chinese Med. 1991;  19 73-78
• 16 Tamaya T, Sato S, Okada H. Possible mechanisms of steroid action of the plant herb extracts glycyrrhizin, glycyrrhetinic acid, and paeoniflorin: inhibition by plant herb extracts of steroid protein binding in the rabbit.  Am J Obstet Gynecol. 1986;  155 1134-1139
• 17 Wang G M, Ge R S, Latif S A, Morris D J, Hardy M P. Expression of 11 beta-hydroxylase in rat Leydig cells.  Endocrinology. 2002;  143 621-626
• 18 Yaginuma T, Izumi R, Yasui H, Arai T, Kawabata M. Effect of traditional herbal medicine on serum testosterone levels and its induction of regular ovulation in hyperandrogenic and oligomenorrheic women.  Acta Obst Gynaec Jpn. 1982;  34 939-944