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Int J Sports Med 2004; 25(6): 446-449
DOI: 10.1055/s-2004-820937
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

D-Amphetamine-Induced Hydrogen Peroxide Production in Skeletal Muscle is Modulated by Monoamine Oxidase Inhibition

J. A. Duarte1 , 5 , F. Carvalho2 , E. Fernandes3 , F. Remião2 , M. L. Bastos2 , J. Magalhães1 , H.-J. Appell4 , 5
  • 1Department of Sport Biology, Faculty of Sport Science, University of Porto, Porto, Portugal
  • 2ICETA/CEQUP, Toxicology Department, Faculty of Pharmacy, University of Porto, Porto, Portugal
  • 3ICETA/CEQUP, Physical Chemistry Department, Faculty of Pharmacy, University of Porto, Porto, Portugal
  • 4Department of Physiology and Anatomy, German Sport University Cologne, Cologne, Germany
  • 5Muscle Atrophy Research Group, Cologne, Germany
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Publication History

Accepted after revision: September 30, 2003

Publication Date:
02 September 2004 (online)

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Abstract

The aim of this paper was to study the influence of d-amphetamine administration as a sympathomimetic drug on the synthesis of hydrogen peroxide (H2O2) in mouse soleus muscle and to investigate the modulating effects of pargyline, an inhibitor of monoamine oxidase (MAO) in this context. Charles River mice were assigned to four groups: Control, d-amphetamine treated, pargyline treated, and amphetamine + pargyline treated. Their soleus muscles were removed 0, 15, 30, 60, and 120 min after treatment. The amount of hydrogen peroxide formation within the muscles was estimated using an indirect method. The control data showed a continuous production of hydrogen peroxidase. Pargyline administration lead to an initial increase of H2O2 production that later faded below control levels. Administration of amphetamine finally stimulated H2O2 production much above control levels. When combining amphetamine and pargyline treatment, H2O2 production was accelerated in the initial phase but dropped to control levels at 30 min. It is concluded that in skeletal muscle MAO is an important source of hydrogen peroxide production triggered by amphetamine administration and that this tissue plays a hitherto not described role in oxidizing circulating biogenic monoamines.

Key words

D-amphetamine - pargyline - hydrogen peroxide - MAO - skeletal muscle

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Prof. Dr. Hans-Joachim Appell

Department of Physiology and Anatomy, German Sport University

50927 Cologne

Germany

Phone: + 492214982543

Fax: + 49 22 14 91 20 01

Email: appell@dshs-koeln.de

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