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Int J Sports Med 2005; 26(7): 552-557
DOI: 10.1055/s-2004-830334
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

Exercise Intensity and Duration Affect Blood Soluble HSP72

E. Fehrenbach1 , A. M. Niess2 , K. Voelker3 , H. Northoff1 , F. C. Mooren3
  • 1Department of Transfusion Medicine, University of Tübingen, Tübingen, Germany
  • 2Medical Clinic and Polyclinic, Department of Sports Medicine, University of Tübingen, Tübingen, Germany
  • 3Institute of Sports Medicine, University Hospital Münster, Münster, Germany
Further Information

Publication History

Accepted after revision: July 31, 2004

Publication Date:
26 November 2004 (online)

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Abstract

Soluble heat shock protein 72 (sHSP72) is suggested to play a role as a signalling molecule in the immune response to exercise. We were interested in whether duration and intensity of endurance running affect the level of inducible sHSP72 in the plasma/serum of endurance athletes. In the first part of the study, the influence of a continuous treadmill run of 60 min (CR) with an intensity of 75 % V·O2max, a long treadmill run of 120 min (LR) with an intensity of 60 % V·O2max, an extensive interval training program (IT; 10 · 1000 m, ca. 35 min, V·O2max 88 %), and a competitive marathon run (MA) within 260 ± 39 min (V·O2max ca. 65 %) on the release of sHSP72 into the peripheral blood was tested. Blood samples were drawn before and directly after exercise, as well as 0.5, 1, 3, 24 h after exercise to determine sHSP72 levels. Secondly, we compared the effects of two exercise bouts with identical duration (23.7 ± 7 min) but different intensities (Exhaustive exercise (ET) at 80 % V·O2max vs. moderate exercise (MT) at 60 % V·O2max) on sHSP72 concentration. The sHSP72 levels in plasma/serum were analyzed using an enzyme immunoassay specific for inducible HSP72 (Stressgen,Victoria, Canada). Early, significant increases of sHSP72 were detected immediately after all types of exercise with highest levels after MA. ET induced significantly higher levels of sHSP72 compared with MT. Long-lasting, competitive endurance exercise induced a more pronounced response of sHSP compared with more intensive but shorter exercise. Exercise intensity was also an important influencing factor. A duration- and intensity-dependent role for sHSP72 in the exercise-induced changes of the immune response may be assumed.

Key words

Heat shock protein - soluble HSP72 - blood - immune system - leukocyte - plasma

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PhD Elvira Fehrenbach

Department of Transfusion Medicine, University Clinic of Tübingen

Otfried-Müller-Straße 4/1

72076 Tübingen

Germany

Phone: + 4970712981637

Fax: + 49 70 71 29 52 40

Email: elvira.fehrenbach@med.uni-tuebingen.de

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