Exercise Intensity and Duration Affect Blood Soluble HSP72

Abstract

Soluble heat shock protein 72 (sHSP72) is suggested to play a role as a signalling molecule in the immune response to exercise. We were interested in whether duration and intensity of endurance running affect the level of inducible sHSP72 in the plasma/serum of endurance athletes. In the first part of the study, the influence of a continuous treadmill run of 60 min (CR) with an intensity of 75 % V·O_2max, a long treadmill run of 120 min (LR) with an intensity of 60 % V·O_2max, an extensive interval training program (IT; 10 · 1000 m, ca. 35 min, V·O_2max 88 %), and a competitive marathon run (MA) within 260 ± 39 min (V·O_2max ca. 65 %) on the release of sHSP72 into the peripheral blood was tested. Blood samples were drawn before and directly after exercise, as well as 0.5, 1, 3, 24 h after exercise to determine sHSP72 levels. Secondly, we compared the effects of two exercise bouts with identical duration (23.7 ± 7 min) but different intensities (Exhaustive exercise (ET) at 80 % V·O_2max vs. moderate exercise (MT) at 60 % V·O_2max) on sHSP72 concentration. The sHSP72 levels in plasma/serum were analyzed using an enzyme immunoassay specific for inducible HSP72 (Stressgen,Victoria, Canada). Early, significant increases of sHSP72 were detected immediately after all types of exercise with highest levels after MA. ET induced significantly higher levels of sHSP72 compared with MT. Long-lasting, competitive endurance exercise induced a more pronounced response of sHSP compared with more intensive but shorter exercise. Exercise intensity was also an important influencing factor. A duration- and intensity-dependent role for sHSP72 in the exercise-induced changes of the immune response may be assumed.

Heat shock protein - soluble HSP72 - blood - immune system - leukocyte - plasma

References

• 1 Appell H J, Soares J M, Duarte J A. Exercise, muscle damage and fatigue.  Sports Med. 1992;  13 108-115
• 2 Asea A. Chaperokine-induced signal transduction pathways.  Exerc Immunol Rev. 2003;  9 25-33
• 3 Asea A, Kraft S K, Kurt-Jones E A, Stevenson M A, Chen L B, Finnberg R W, Koo G C, Calderwood S K. HSP70 stimulates cytokine production through a CD14-dependant pathway, demonstrating its dual role as a chaperone and cytokine.  Nat Med. 2000;  6 435-442
• 4 Basu S, Binder R J, Suto R, Anderson K M, Srivastava P K. Necrotic but not apoptotic cell death releases heat shock proteins, which deliver a partial maturation signal to dendritic cells and activate the NF-kappa B pathway.  Int Immunol. 2000;  12 1539-1546
• 5 Binder R J, Blachere N E, Srivastava P K. Heat shock protein-chaperoned peptides but not free peptides introduced into the cytosol are presented efficiently by major histocompatibility complex I molecules.  J Biol Chem. 2001;  276 17163-17171
• 6 Campisi J, Fleshner M. Role of extracellular HSP72 in acute stress-induced potentiation of innate immunity in active rats.  J Appl Physiol. 2003;  94 43-52
• 7 Dill D B, Costill D L. Calculation of percentage changes in volumes of blood plasma and red cells in dehydration.  J Appl Physiol. 1974;  37 247-248
• 8 Febbraio M A, Ott P, Nielsen H B, Steensberg A, Keller C, Krustrup P, Secher N H, Pedersen B K. Exercise induces hepatosplanchnic release of heat shock protein 72 in humans.  J Physiol. 2002;  544 957-962
• 9 Febbraio M A, Pedersen B K. Muscle-derived interleukin-6: mechanisms for activation and possible biological roles.  FASEB J. 2002;  16 1335-1347
• 10 Febbraio M A, Steensberg A, Walsh R, Koukoulas I, van Hall G, Saltin B, Pedersen B K. Reduced glycogen availability is associated with an elevation in HSP72 in contracting human skeletal muscle.  J Physiol. 2002;  538 911-917
• 11 Fehrenbach E, Niess A M. Role of heat shock proteins in the exercise response.  Exerc Immunol Rev. 1999;  5 57-77
• 12 Fehrenbach E, Niess A M, Dickhuth H-H, Northoff H. Influence of exercise, heat and hypoxia on plasma-HSP72.  Eur J Physiol (Pfluegers Arch). 2002;  443 362
• 13 Fehrenbach E, Niess A M, Passek F, Sorichter S, Schwirtz A, Berg A, Dickhuth H-H, Northoff H. Influence of different types of exercise on the expression of haem oxygenase-1 in leukocytes.  J Sport Sci. 2003;  21 383-389
• 14 Fehrenbach E, Niess A M, Schlotz E, Passek F, Dickhuth H-H, Northoff H. Transcriptional and translational regulation of heat shock proteins (HSP27, HSP70) in leukocytes of endurance runners.  J Appl Physiol. 2000;  89 704-710
• 15 Fehrenbach E, Niess A M, Veith R, Dickhuth H-H, Northoff H. Changes of HSP72-expression in leukocytes are associated with adaptation to exercise under conditions of high environmental temperature.  J Leuk Biol. 2001;  69 747-754
• 16 Fehrenbach E, Passek F, Niess A M, Pohla H, Weinstock C, Dickhuth H-H, Northoff H. HSP expression in human leucocytes is modulated by endurance exercise.  Med Sci Sports Exerc. 2000;  32 592-600
• 17 Guzhova I V, Arnholdt A C, Darieva Z A, Kinev A V, Lasunskaia E B, Nilsson K, Bozhkov V M, Voronin A P, Margulis B A. Effects of exogenous stress protein 70 on the functional properties of human promonocytes through binding to cell surface and internalization.  Cell Stress Chaperones. 1998;  3 67-77
• 18 Kilgore J L, Musch T I, Ross C R. Physical activity, muscle, and the HSP70 response.  Can J Appl Physiol. 1998;  23 245-260
• 19 Kuppner M C, Gastpar R, Gelwer S, Nossner E, Ochmann O, Scharner A, Issels R D. The role of heat shock protein (hsp70) in dendritic cell maturation: hsp70 induces the maturation of immature dendritic cells but reduces DC differentiation from monocyte precursors.  Eur J Immunol. 2001;  31 1602-1609
• 20 Liu Y, Lormes W, Baur C, Opitz-Gress A, Altenburg D, Lehmann M, Steinacker J M. Human skeletal muscle HSP70 response to physical training depends on exercise intensity.  Int J Sports Med. 2000;  21 351-355
• 21 Liu Y, Mayr S, Opitz-Gress A, Zeller C, Lormes W, Baur S, Lehmann M, Steinacker J M. Human skeletal muscle HSP70 response to training in highly trained rowers.  J Appl Physiol. 1999;  86 101-104
• 22 Locke M, Noble E G. Stress proteins: the exercise response.  Can J Appl Physiol. 1995;  20 155-167
• 23 Locke M, Noble E G, Atkinson B G. Exercising mammals synthesize stress proteins.  Am J Physiol. 1990;  258 723-729
• 24 Locke M, Tanguay R M, Klabunde R E, Ianuzzo C D. Enhanced postischemic myocardial recovery following exercise induction of HSP72.  Am J Physiol. 1995;  269 320-325
• 25 McConnell T R. Practical considerations in the testing of V·O_2max in runners.  Sports Med. 1988;  5 57-68
• 26 Mestril R, Chi S H, Sayen M R, Dillmann W H. Isolation of a novel inducible rat heat-shock protein (HSP70) gene and its expression during ischaemia/hypoxia and heat shock.  Biochem J. 1994;  298 561-569
• 27 Millar D G, Garza K M, Odermatt B, Elford A R, Ono N, Li Z, Ohashi P S. Hsp70 promotes antigen-presenting cell function and converts T-cell tolerance to autoimmunity in vivo.  Nat Med. 2003;  9 1469-1476
• 28 Milne K J, Noble E G. Exercise-induced elevation of HSP70 is intensity dependent.  J Appl Physiol. 2002;  93 561-568
• 29 Morimoto R I, Tissieres A, Georgopoulos C. The Biology of Heat Shock Proteins and Molecular Chaperones. New York; Cold Spring Harbor Laboratory Press 1994
• 30 Moseley P L. Exercise, stress, and the immune conversation.  Exerc Sport Sci Rev. 2000;  28 128-132
• 31 Northoff H, Enkel S, Weinstock C. Exercise, injury, and immune function.  Exerc Immunol Rev. 1995;  1 1-25
• 32 Pedersen B K, Steensberg A. Exercise and hypoxia: effects on leukocytes and interleukin-6-shared mechanisms?.  Med Sci Sports Exerc. 2002;  34 2004-2013
• 33 Ryan A J, Gisolfi C V, Moseley P L. Synthesis of 70 K stress protein by human leukocytes: effect of exercise in the heat.  J Appl Physiol. 1991;  70 466-471
• 34 Salo D C, Donovan C M, Davies K J. HSP70 and other possible heat shock or oxidative stress proteins are induced in skeletal muscle, heart, and liver during exercise.  Free Radic Biol Med. 1991;  11 239-246
• 35 Schneider E M, Niess A M, Lorenz I, Northoff H, Fehrenbach E. Inducible HSP70 expression analysis after heat and physical exercise - Transcriptional, protein expression and subcellular localization.  Am NY Acad Sci. 2002;  973 8-12
• 36 Skidmore R, Gutierrez J A, Guerriero Jr V, Kregel K C. HSP70 induction during exercise and heat stress in rats: role of internal temperature.  Am J Physiol. 1995;  268 92-97
• 37 Smith L L. Acute inflammation: the underlying mechanism in delayed onset muscle soreness?.  Med Sci Sports Exerc. 1991;  23 542-551
• 38 Sondermann H, Becker T, Mayhew M, Wieland F, Hartl F U. Characterization of a receptor for heat shock protein 70 on macrophages and monocytes.  Biol Chem. 2000;  381 1165-1174
• 39 Srivastava P K, Menoret A, Basu S, Binder R J, McQuade K L. Heat shock proteins come of age: primitive functions acquire new roles in an adaptive world.  Immunity. 1998;  8 657-665
• 40 Tidball J G. Inflammatory cell response to acute muscle injury.  Med Sci Sports Exerc. 1995;  27 1022-1032
• 41 Walsh R C, Koukoulas I, Garnham A, Moseley P L, Hargreaves M, Febbraio M A. Exercise increases serum Hsp72 in humans.  Cell Stress Chaperones. 2001;  6 386-393
• 42 Walters T J, Ryan K L, Tehrany M R, Jones M B, Paulus L A, Mason P A. HSP70 expression in the CNS in response to exercise and heat stress in rats.  J Appl Physiol. 1998;  84 1269-1277
• 43 Weinstock C, Konig D, Harnischmacher R, Keul J, Berg A, Northoff H. Effect of exhaustive exercise stress on the cytokine response.  Med Sci Sports Exerc. 1997;  29 345-354