Endogenous Cytokinin Oscillations Control Cell Cycle Progression of Tobacco BY-2 Cells

K. Hartig; E. Beck

doi:10.1055/s-2004-830474

Plant Biology, Table of Contents

Plant Biol (Stuttg) 2005; 7(1): 33-40
DOI: 10.1055/s-2004-830474

Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Endogenous Cytokinin Oscillations Control Cell Cycle Progression of Tobacco BY-2 Cells

K. Hartig¹ , E. Beck¹

¹Department of Plant Physiology, University of Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany

Abstract

The significance of cytokinins for the progression of the cell cycle is well known. Cytokinins contribute to the control of the expression of D-cyclins and other cell cycle genes, but knowledge as to how they affect the progression of the cell cycle is still limited. Highly synchronized tobacco BY-2 cells with clearly defined cell cycle stages were employed to determine cytokinin patterns in detail throughout the entire cycle. Concentrations of trans-zeatin, and of some other cytokinins, oscillated during the course of the cell cycle, increasing substantially at all four phase transitions and decreasing again to a minimum value during the course of each subsequent phase. Addition of exogenous cytokinins or inhibition of cytokinin biosynthesis promoted the progression of the cell cycle when the effects of these manipulations intensified the endogenous fluctuations, whereas the progression of the cycle was retarded when the amplitude of the fluctuations was decreased. The results show that the attainment of low concentrations of cytokinins is as important as the transient increases in concentration for a controlled progression from one phase of the cell cycle to the next. Cytokinin oxidase/dehydrogenase activity also showed fluctuations during the course of the cell cycle, the timing of which could at least partly explain oscillations of cytokinin levels. The activities of the enzyme were sufficient to account for the rates of cytokinin disappearance observed subsequent to a phase transition.

Key words

Cytokinins - cell cycle - BY-2 cells - lovastatin - cytokinin oxidase/dehydrogenase

Full Text

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K. Hartig

Department of Plant Physiology
University of Bayreuth

Universitätsstraße 30

95440 Bayreuth

Germany

Email: katja.hartig@uni-bayreuth.de

Editor: R. Mendel