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Plant Biol (Stuttg) 2006; 8(5): 698-705
DOI: 10.1055/s-2006-924150
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Chlorophyll a Fluorescence Imaging of Ozone-Stressed Brassica napus L. Plants Differing in Glucosinolate Concentrations

B. Gielen1 , K. Vandermeiren2 , N. Horemans3 , D. D'Haese3 , 4 , R. Serneels5 , R. Valcke6
  • 1Department of Biology, Research Group of Plant and Vegetation Ecology, University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, 2610 Wilrijk, Belgium
  • 2Veterinary and Agrochemical Research Centre, Leuvensesteenweg 13, 3080 Tervuren, Belgium
  • 3Department of Biology, Research Group of Plant Physiology, University of Antwerp, Campus Middelheim, Groenenborgerlaan 171, 2020 Antwerpen, Belgium
  • 4School of Biology and Psychology, University of Newcastle, Devonshire Terrace - Devonshire Building, Newcastle-Upon-Tyne, NE1 7RU, UK
  • 5Department WNI, Theoretical Physics, Hasselt University, Agoralaan, Bldg D, 3590 Diepenbeek, Belgium
  • 6Centre for Environmental Siences, Dept. SBG, Laboratory of Molecular and Physical Plant Physiology, Hasselt University, Agoralaan, Bldg D, 3590 Diepenbeek, Belgium
Further Information

Publication History

Received: November 3, 2005

Accepted: March 23, 2006

Publication Date:
05 July 2006 (online)

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Abstract

Brassicaceae are characterised by glucosinolates (GS), which appear to be involved not only in biotic but also in abiotic stress responses of plants. We investigated the effect of O3 stress on leaf GS concentrations in two lines of Brassica napus L., differing in GS content. Ozone fumigation decreased GS concentrations in leaves of B. napus of one line. In control conditions, chlorophyll content, rates of saturating photosynthesis, and quantum yield of photosystem 2 differed between the two Brassica lines, but differences were smaller in O3‐stress conditions, suggesting that the relationship between leaf GS concentration and sensitivity to abiotic stress merits further research. In agreement with other ecophysiological measurements, chlorophyll fluorescence imaging clearly distinguished both lines and in some cases also treatments. A method for analysis of fluorescence images accounting for the two-dimensional leaf heterogeneity is presented.

Key words

Image processing - gas exchange - quantum yield of photosystem 2.

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B. Gielen

Department of Biology
Research Group of Plant and Vegetation Ecology
University of Antwerp
Campus Drie Eiken

Universiteitsplein 1

2610 Wilrijk

Belgium

Email: birgit.gielen@ua.ac.be

Editor: B. Demmig-Adams

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