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Int J Sports Med 1992; 13: S155-S157
DOI: 10.1055/s-2007-1024624
© Georg Thieme Verlag Stuttgart · New York

Metabolic Transient Studies by NMR

T. Binzoni1 , 4 , G. Ferretti1 , K. Schenker2 , F. Barbalat3 , E. Hiltbrand4 , P. Cerretelli1
  • 1Dépt. de Physiologie, CMU, Genève, Switzerland
  • 2Spectrospin AG, Fällanden, Switzerland
  • 3Dépt. de Chimie Pharmaceutique, Faculté des Sciences, Genève, Switzerland
  • 4Dépt. de Radiologie, HCUG, Genève, Switzerland
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Publication Date:
14 March 2008 (online)

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Abstract

The time course of phosphocreatine (PC) hydrolysis in humans was measured by 31P-NMR spectroscopy (31P-NMRS) with a time resolution of 10.8 s in the gastrocnemius muscle and a relationship between muscle O2 consumption (V̇O2) and [PC] was derived from a bio-energetic model. This allowed a direct estimate of the half-time of the intracellular V̇O2 kinetics (t1/2 V̇O2) of the contracting human gastrocnemius in aerobic conditions. t1/2 V̇O2 was found to be ∼ 16 s and independent of the work load. This value corresponds to the shortest t1/2 V̇O2 determined at the mouth of the subject in the absence of lactate accumulation in the rest to work transient. t1/2 V̇O2 may now be assessed in man at low muscle temperatures. To this aim a procedure was developed allowing corrections of the 31P-NMR spectra based on the muscle temperature profiles obtained by a simultaneously acquired proton image.

Key words

Exercise - phosphocreatine - O2 consumption - temperature - muscle - 31P-NMR - humans