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Int J Sports Med 2008; 29(5): 372-378
DOI: 10.1055/s-2007-965335
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

Haemoglobin Mass in Cyclists during Stage Racing

Y. O. Schumacher1 , T. Pottgiesser1 , C. Ahlgrim1 , S. Ruthardt1 , H.-H. Dickhuth1 , K. Roecker1
  • 1Abteilung Sportmedizin, Medizinische Universitätsklinik Freiburg, Freiburg, Germany
Further Information

Publication History

accepted after revision April 3, 2007

Publication Date:
05 July 2007 (online)

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Abstract

Haemoglobin mass is a main determinant of maximal oxygen uptake. Blood doping aims at increasing this variable. Limits for haematocrit and haemoglobin concentration are used as indicators of blood doping. However, these variables are measures of concentration, do not represent total haemoglobin mass and are altered by vascular volumes shifts. Direct estimation of haemoglobin mass could improve blood tests. It is unknown if physical exercise alters haemoglobin mass. The purpose of this study was to investigate the reaction of haemoglobin mass and other vascular compartments to heavy exercise in athletes. Haemoglobin mass and vascular compartments were evaluated using the optimised CO rebreathing method in 7 elite cyclists during a stage race. Simultaneously, haemoglobin concentration and haematocrit were analysed. Haemoglobin mass (pre-race 958 ± 123 g, end race 948 ± 106 g) and red cell volume did not change significantly over the study period, while plasma volume and blood volume tended to increase. Haematocrit (pre-race 44.1 ± 2.5 %, end race 40.9 ± 1.59 %) and haemoglobin concentration (pre race 15.8 ± 0.9 g/dl, end race 14.7 ± 0.7 g/dl) decreased. During the study, a plasma volume expansion as adaptation to prolonged exercise occurred. Haemoglobin concentration and haematocrit decreased accordingly, whereas haemoglobin mass remained stable. Haemoglobin mass might therefore be a suitable screening tool for blood manipulations.

Key words

exercise - blood doping - carbon monoxide rebreathing

References

  • 1 Ashenden M, Varlet-Marie E, Lasne F, Audran M. The effects of microdose recombinant human erythropoietin regimens in athletes.  Haematologica. 2006;  91 1143-1144
    PubMedGoogle Scholar
  • 2 Bassett Jr D R, Howley E T. Limiting factors for maximum oxygen uptake and determinants of endurance performance.  Med Sci Sports Exerc. 2000;  32 70-84
    PubMedGoogle Scholar
  • 3 Bland J M, Altman D G. Measurement error.  BMJ. 1996;  313 744
    PubMedGoogle Scholar
  • 4 Burge C M, Skinner S L. Determination of hemoglobin mass and blood volume with CO: evaluation and application of a method.  J Appl Physiol. 1995;  79 623-631
    PubMedGoogle Scholar
  • 5 Ekblom B, Berglund B. Effect of erythropoietin administration on maximal aerobic power.  Scand Med Sci Sports. 1991;  1 88-93
    PubMedGoogle Scholar
  • 6 Gledhill N. Blood doping and related issues: a brief review.  Med Sci Sports Exerc. 1982;  14 183-189
    PubMedGoogle Scholar
  • 7 Gore C J, Hopkins W G, Burge C M. Errors of measurement for blood volume parameters: a meta-analysis.  J Appl Physiol. 2005;  99 1745-1758
    CrossrefPubMedGoogle Scholar
  • 8 Gore C J, Parisotto R, Ashenden M J, Stray-Gundersen J, Sharpe K, Hopkins W, Emslie K R, Howe C, Trout G J, Kazlauskas R, Hahn A G. Second-generation blood tests to detect erythropoietin abuse by athletes.  Haematologica. 2003;  88 333-344
    PubMedGoogle Scholar
  • 9 Harris E K. Effects of intra- and interindividual variation on the appropriate use of normal ranges.  Clin Chem. 1974;  20 1535-1542
    PubMedGoogle Scholar
  • 10 Heinicke K, Wolfarth B, Winchenbach P, Biermann B, Schmid A, Huber G, Friedmann B, Schmidt W. Blood volume and hemoglobin mass in elite athletes of different disciplines.  Int J Sports Med. 2001;  22 504-512
    Article in Thieme ConnectPubMedGoogle Scholar
  • 11 Hopkins W G. Measures of reliability in sports medicine and science.  Sports Med. 2000;  30 1-15
    CrossrefPubMedGoogle Scholar
  • 12 Katch V L, Sady S S, Freedson P. Biological variability in maximum aerobic power.  Med Sci Sports Exerc. 1982;  14 21-25
    PubMedGoogle Scholar
  • 13 Lasne F, Martin L, Crepin N, de Ceaurriz J. Detection of isoelectric profiles of erythropoietin in urine: differentiation of natural and administered recombinant hormones.  Analyt Biochem. 2002;  311 119-126
    CrossrefPubMedGoogle Scholar
  • 14 Malcovati L, Pascutto C, Cazzola M. Hematologic passport for athletes competing in endurance sports: a feasibility study.  Haematologica. 2003;  88 570-581
    PubMedGoogle Scholar
  • 15 Myers J, Walsh D, Sullivan M, Froelicher V. Effect of sampling on variability and plateau in oxygen uptake.  J Appl Physiol. 1990;  68 404-410
    PubMedGoogle Scholar
  • 16 Nelson M, Popp H, Sharpe K, Ashenden M. Proof of homologous blood transfusion through quantification of blood group antigens.  Haematologica. 2003;  88 1284-1295
    PubMedGoogle Scholar
  • 17 Odeh R E, Owen D B. Tables for Normal Tolerance Limits, Sampling Plans, and Screening. New York; Marcel Dekker Inc. 1980
    Google Scholar
  • 18 Parisotto R, Wu M, Ashenden M J, Emslie K R, Gore C J, Howe C, Kazlauskas R, Sharpe K, Trout G J, Xie M. Detection of recombinant human erythropoietin abuse in athletes utilizing markers of altered erythropoiesis.  Haematologica. 2001;  86 128-137
    PubMedGoogle Scholar
  • 19 Sottas P E, Robinson N, Giraud S, Taroni F, Kamber M, Mengin P. Statistical classification of abnormal blood profiles in athletes.  Int J Biostat. 2006;  2 1011
    PubMedGoogle Scholar
  • 20 Sawka M N, Convertino V A, Eichner E R, Schnieder S M, Young A J. Blood volume: importance and adaptations to exercise training, environmental stresses, and trauma/sickness.  Med Sci Sports Exerc. 2000;  32 332-348
    CrossrefPubMedGoogle Scholar
  • 21 Sawka M N, Young A J, Pandolf K B, Dennis R C, Valeri C R. Erythrocyte, plasma, and blood volume of healthy young men.  Med Sci Sports Exerc. 1992;  24 447-453
    PubMedGoogle Scholar
  • 22 Schmidt W, Biermann B, Winchenbach P, Lison S, Boning D. How valid is the determination of hematocrit values to detect blood manipulations?.  Int J Sports Med. 2000;  21 133-138
    Article in Thieme ConnectPubMedGoogle Scholar
  • 23 Schmidt W, Prommer N. The optimised CO-rebreathing method: a new tool to determine total haemoglobin mass routinely.  Eur J Appl Physiol. 2005;  95 486-495
    CrossrefPubMedGoogle Scholar
  • 24 Schumacher Y O, Grathwohl D, Barturen J M, Wollenweber M, Heinrich L, Schmid A, Huber G, Keul J. Haemoglobin, haematocrit and red blood cell indices in elite cyclists. Are the control values for blood testing valid?.  Int J Sports Med. 2000;  21 380-385
    Article in Thieme ConnectPubMedGoogle Scholar
  • 25 Schumacher Y O, Temme J, Bueltermann D, Schmid A, Berg A. The influence of exercise on serum markers of altered erythropoiesis and the indirect detection models of recombinant human erythropoietin abuse in athletes.  Haematologica. 2003;  88 712-714
    PubMedGoogle Scholar
  • 26 Sharpe K, Ashenden M J, Schumacher Y O. A third generation approach to detect erythropoietin abuse in athletes.  Haematologica. 2006;  91 356-363
    PubMedGoogle Scholar
  • 27 Sharpe K, Hopkins W, Emslie K R, Howe C, Trout G J, Kazlauskas R, Ashenden M J, Gore C, Parisotto R, Hahn A. Development of reference ranges in elite athletes for markers of altered erythropoiesis.  Haematologica. 2002;  87 1248-1257
    PubMedGoogle Scholar
  • 28 Sklerov J H, Goldbaum L R. Mechanism of carbon monoxide toxicity. (Part 1).  FASEB Journal. 1999;  13 A155
    PubMedGoogle Scholar
  • 29 UCI .UCI Sporting Safety and Condition Regulations. Lausanne; UCI 1997 Chapter XIII, 13.01.023
    Google Scholar
  • 30 Weight L M, Darge B L, Jacobs P. Athletes' pseudoanaemia.  Eur J Appl Physiol. 1991;  62 358-362
    CrossrefPubMedGoogle Scholar
  • 31 Williams G Z. Individuality of clinical biochemical patterns in preventive health maintenance.  J Occup Med. 1967;  9 567-570
    PubMedGoogle Scholar
  • 32 Zander R, Schaffartzik W. Haem oxymeter.  Qualitest. 1999;  5 1-8
    PubMedGoogle Scholar

PD Dr. med. Yorck Olaf Schumacher

Abteilung Sportmedizin
Medizinische Universitätsklinik Freiburg

Hugstetter Straße 55

79106 Freiburg

Germany

Phone: + 49 76 12 70 74 83

Fax: + 49 76 12 70 74 70

Email: olaf@msm1.ukl.uni-freiburg.de

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