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Horm Metab Res 2007; 39(3): 192-196
DOI: 10.1055/s-2007-970417
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Site-specific Effect of Estradiol on Gene Expression in the Adipose Tissue of ob/ob Mice

S. Shinozaki 1 , T. Chiba 1 , K. Kokame 2 , T. Miyata 2 , M. Ai 1 , A. Kawakami 1 , E. Kaneko 1 , M. Yoshida 1 , K. Shimokado 1
  • 1Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School, Yushima Bunkyo-ku, Tokyo, Japan
  • 2National Cardiovascular Center Research Institute, Fujishirodai, Suita, Osaka, Japan
Weitere Informationen

Publikationsverlauf

received 6. 7. 2006

accepted 31. 10. 2006

Publikationsdatum:
20. März 2007 (online)

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Abstract

Sex hormones are likely to be involved in sex differences in adipose tissue distribution. To test whether estrogen regulates genes expressed in the adipose tissue in a site-specific manner, we studied the effect of exogenous estradiol on the gene expression in visceral and subcutaneous adipose tissues of male ob/ob mice. We screened genes expressed in a site- and sex-specific manner, and genes that were affected by exogenous estradiol by DNA chip analysis. They were verified by real-time PCR. Myosin heavy chain 2B (Myh4) and phosphoglycerate mutase muscle-specific subunit (Pgam) were expressed specifically in the subcutaneous adipose tissue, and uroplakin IIIb (UP3) was expressed specifically in the visceral adipose tissue. DEAD-box Y RNA helicase (DBY) and eukaryotic initiation factor 2 gamma Y (eIF2γ Y) were expressed only in male adipose tissue. X-chromosome inactive specific transcript (Xist) was expressed only in female adipose tissue. When estradiol was subcutaneously administrated to male mice, the expression of monocyte-chemoattractant protein-1 (MCP-1) and androgen receptor (AR) genes was regulated in a site-specific manner. The difference in the amount of estrogen receptor did not account for the site-specific effect of estrogen. Our findings show that estrogen affects the expression of some adipocyte genes in a site-specific manner.

Key words

subcutaneous adipose tissue - visceral adipose tissue - estrogen - gene chip - estrogen receptors - adipose tissue distribution

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Correspondence

K. Shimokado

Geriatrics and Vascular Medicine · Tokyo Medical and Dental University Graduate School

1-5-45 Yushima Bunkyo-ku

Tokyo 113-8519

Japan

Telefon: +81/3/58 03 59 68

Fax: +81/3/58 03 02 67

eMail: k.shimoka.vasc@tmd.ac.jp

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