Download PDF
Horm Metab Res 2007; 39(7): 501-506
DOI: 10.1055/s-2007-982515
Original Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Control by Reproduction-related Hormones of Resistin Expression and Plasma Concentration

S. Caja 1 , M. Puerta 1
  • 1Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain
Further Information

Publication History

received 24.10.2006

accepted 22.12.2006

Publication Date:
05 July 2007 (online)

Also available at
    Permissions and Reprints

Abstract

Although not simultaneously, resistin expression in white adipose tissue (WAT) and resistin plasma concentration have been shown to increase in pregnant rats. To clarify the involvement of sex hormones in such increases, we administered for 3-5 days progesterone, estradiol, or human chorionic gonadotropin (hCG) to female rats in dioestrus II. Progesterone increased resistin expression retroperitoneal WAT but lacked effect in parametrial or subcutaneous depots. It also increased resistin plasma concentration. Estradiol decreased resistin expression in both parametrial and inguinal WAT but was without effect on retroperitoneal depots. It did not alter plasma resistin. Human hCG increased resistin expression in all the visceral depots examined - parametrial, inguinal and retroperitoneal - but did not change plasma resistin. These results show that hormonal influences in resistin expression are depot-dependent and can run separately from changes in its plasma concentration. Besides, the locally restricted effect of progesterone in resistin expression compared with that of hCG suggests it is not the only hormone enhancing resistin expression in early pregnancy. However, it could enhance resistin release in late pregnancy. Estradiol could be involved in the decrease of resistin expression in late pregnancy. Finally, since hCG acts through LH receptors, our results suggest that they are present in WAT and that they control resistin expression.

Key words

Estradiol - progesterone - chorionic gonadotropin - white adipose tissue

References

  • 1 Kim KH, Lee K, Moon YS, Sul HS. A cysteine-rich adipose tissue-specific secretory factor inhibits adipocyte differentiation.  J Biol Chem. 2001;  276 11252-11256
    CrossrefPubMedGoogle Scholar
  • 2 Steppan CM, Bailey ST, Bhat S, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA. The hormone resistin links obesity to diabetes.  Nature. 2001;  409 307-312
    CrossrefPubMedGoogle Scholar
  • 3 Kusminski CM, MacTernan PG, Kumar S. Role of resistin in obesity, insulin resistance and type II diabetes.  Clin Sci (Lond). 2005;  109 243-256
    PubMedGoogle Scholar
  • 4 Schäffler A, Büchler C, Müller-Ladner U, Herfarth H, Ehling A, Paul G, Schölmerich J, Zietz B. Identification of variables influencing resistin serum levels in patients with type 1 and type 2 diabetes mellitus.  Horm Metab Res. 2004;  36 702-707
    Article in Thieme ConnectPubMedGoogle Scholar
  • 5 Bing C, Gomez-Ambrosi J, Zabalegui N, Williams G, Trayhurn P. Resistin and RELM-alpha gene expression in white adipose tissue of lactating mice.  Biochem Biophys Res Commun. 2002;  296 458-462
    CrossrefPubMedGoogle Scholar
  • 6 Puerta M, Abelenda M, Rocha M, Trayhurn P. Effect of acute cold exposure on the expression of the adiponectin, resistin and leptin genes in rat white and brown adipose tissues.  Horm Metab Res. 2002;  34 629-634
    Article in Thieme ConnectPubMedGoogle Scholar
  • 7 Gomez-Ambrosi J, Zabalegui N, Bing C, Tisdale MJ, Trayhurn P, Williams G. Weight loss in tumour-bearing mice is not associated with changes in resistin gene expression in white adipose tissue.  Horm Metab Res. 2002;  34 674-677
    Article in Thieme ConnectPubMedGoogle Scholar
  • 8 Caja S, Martínez I, Abelenda M, Puerta M. Resistin expression and plasma concentration peak at different times during pregnancy in rats.  J Endocrinol. 2005;  185 551-559
    CrossrefPubMedGoogle Scholar
  • 9 Lee JH, Bullen Jr JW, Stoyneva VL, Mantzoros CS. Circulating resistin in lean, obese, and insulin-resistant mouse models: lack of association with insulinemia and glycemia.  Am J Physiol Endocrinol Metab. 2005;  288 E625-E632
    PubMedGoogle Scholar
  • 10 Rajala MW, Qi Y, Patel HR, Takahashi N, Banerjee R, Pajvani UB, Sinha MK, Gingerich RL, Scherer PE, Ahima RS. Regulation of resistin expression and circulating levels in obesity, diabetes, and fasting.  Diabetes. 2004;  53 1671-1679
    CrossrefPubMedGoogle Scholar
  • 11 Gui Y, Silha JV, Murphy LJ. Sexual dimorphism and regulation of resistin, adiponectin, and leptin expression in the mouse.  Obes Res. 2004;  12 1481-1491
    PubMedGoogle Scholar
  • 12 Niswender GD, Juengel JL, Silva PJ, Rollyson MK, MacIntush EW. Mechanisms controlling the function and life span of the corpus luteum.  Physiol Rev. 2000;  80 1-29
    CrossrefPubMedGoogle Scholar
  • 13 Leymarie P, Martal J. Du corps jaune cyclique au corps jaune gestatif. Du corps jaune cyclique au corps jaune gestatif. Thibault C, Levasseur M. Paris: INRA Editions 2001
    Google Scholar
  • 14 Freeman ME. The neuroendocrine control of the ovarian cycle in the rat. The neuroendocrine control of the ovarian cycle in the rat. Knobil E, Neil J. New York: Prentice Hall 1994: 613-658
    Google Scholar
  • 15 Freemark M, Kirk K, Pihoker C, Robertson MC, Shiu RP, Driscoll P. Pregnancy lactogens in the rat conceptus and fetus: circulating levels, distribution of binding, and expression of receptor messenger ribonucleic acid.  Endocrinology. 1993;  133 1830-1842
    CrossrefPubMedGoogle Scholar
  • 16 Lu C-C, Tsai S-C, Wang S-W, Tsai C-L, Lau C-P, Shih H-C, Chen Y-H, Chiao Y-C, Liaw C, Wang PS. Effects of ovarian steroid hormones and thyroxine on calcitonin secretion in pregnant rats.  Am J Physiol. 1998;  274 E246-E252
    PubMedGoogle Scholar
  • 17 Shibata K, Hosoda H, Kojima M, Kangawa K, Makino Y, Makino I, Kawarabayashi T, Futagami K, Gomita Y. Regulation of ghrelin secretion during pregnancy and lactation in the rat: possible involvement of hypothalamus.  Peptides. 2004;  25 279-287
    CrossrefPubMedGoogle Scholar
  • 18 Chern BY, Chen YH, Hong LS, Lapolt PS. Ovarian steroidogenic responsiveness to exogenous gonadotropin stimulation in young and middle-aged female rats.  Proc Soc Exp Biol Med. 2000;  224 285-291
    CrossrefPubMedGoogle Scholar
  • 19 Ghosh S, Misro M, Das UB, Maiti R, Debnath JM, Ghosh D. Effect of human chorionic gonadotrophin coadministration on ovarian steroidogenic and folliculogenic activities in cyclophosphamide treated albino rats.  Reprod Toxicol. 2001;  15 221-225
    CrossrefPubMedGoogle Scholar
  • 20 Rocha M, Bing C, Williams G, Puerta M. Physiologic estradiol levels enhance hypothalamic expression of the long form of the leptin receptor in intact rats.  J Nutr Biochem. 2004;  15 328-334
    PubMedGoogle Scholar
  • 21 Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.  Anal Biochem. 1987;  162 156-159
    PubMedGoogle Scholar
  • 22 Trayhurn P, Duncan JS, Nestor A, Thomas MEA, Rayner DV. Chemiluminescent detection of mRNAs on northern blots with digoxigenin end-labelled oligonucleotides.  Anal Biochem. 1994;  222 224-230
    CrossrefPubMedGoogle Scholar
  • 23 Trayhurn P, Duncan JS, Rayner DV. Acute cold-induced suppression of ob (obese) gene expression in white adipose tissue of mice: mediation by the sympathetic system.  Biochem J. 1995;  311 729-733
    CrossrefPubMedGoogle Scholar
  • 24 Chen L, Nyomba BL. Glucose intolerance and resistin expression in rat offspring exposed to ethanol in utero: modulation by postnatal high-fat diet.  Endocrinology. 2003;  144 500-508
    CrossrefPubMedGoogle Scholar
  • 25 Huang SW, Seow KM, Ho LT, Chien Y, Chung DY, Chang CL, Lai YH, Hwang JL, Juan CC. Resistin mRNA levels are downregulated by estrogen in vivo and in vitro.  FEBS Lett. 2005;  579 449-454
    CrossrefPubMedGoogle Scholar
  • 26 Mayes JS, Watson GH. Direct effects of sex steroid hormones on adipose tissues and obesity.  Obes Rev. 2004;  5 197-216
    CrossrefPubMedGoogle Scholar
  • 27 Rodriguez-Cuenca S, Monjo M, Proenza AM, Roca P. Depot differences in steroid receptor expression in adipose tissue: possible role of the local steroid milieu.  Am J Physiol Endocrinol Metab. 2005;  288 E200-E207
    PubMedGoogle Scholar
  • 28 Nogueiras R, Gualillo O, Caminos JE, Casanueva FF, Dieguez C. Regulation of resistin by gonadal, thyroid hormone, and nutritional status.  Obes Res. 2003;  11 408-414
    CrossrefPubMedGoogle Scholar
  • 29 Chen YH, Lee MJ, Chang HH, Hung PF, Kao YH. 17 beta-estradiol stimulates resistin gene expression in 3T3-L1 adipocytes via the estrogen receptor, extracellularly regulated kinase, and CCAAT/enhancer binding protein-alpha pathways.  Endocrinology. 2006;  147 4496-4504
    CrossrefPubMedGoogle Scholar
  • 30 Costagliola S, Urizar E, Mendive F, Vassart G. Specificity and promiscuity of gonadotropin receptors.  Reproduction. 2005;  130 275-281
    CrossrefPubMedGoogle Scholar
  • 31 Ascoli M, Fanelli F, Segaloff DL. The lutropin/choriogonadotropin receptor, a 2002 perspective.  Endocr Rev. 2002;  23 141-174
    CrossrefPubMedGoogle Scholar
  • 32 Lei ZM, Mishra S, Zou W, Xu B, Foltz M, Li X, Rao CV. Targeted disruption of luteinizing hormone/human chorionic gonadotropin receptor gene.  Mol Endocrinol. 2001;  15 184-200
    CrossrefPubMedGoogle Scholar
  • 33 Rao CV. Multiple novel roles of luteinizing hormone.  Fertil Steril. 2001;  76 1097-1100
    CrossrefPubMedGoogle Scholar
  • 34 Schaffler A, Binart N, Scholmerich J, Buchler C. Hypothesis paper Brain talks with fat-evidence for a hypothalamic-pituitary-adipose axis?.  Neuropeptides. 2005;  39 363-367
    CrossrefPubMedGoogle Scholar

Correspondence

M. Puerta

Department of Animal Physiology II

Faculty of Biological Sciences

Complutense University

José Antonio Novais 2

28040 Madrid

Spain

Phone: +34/91/394 49 90

Fax: +34/91/394 49 35

Email: mpuerta@bio.ucm.es

      >