Abstract
Introduction: Many factors and mechanisms have been proposed as causes for intussusception (IN);
however, the etiology remains unclear. Inflammatory mediators such as tumor necrosis
factor (TNF) and interleukin-6 (IL-6), which are elevated during infectious diseases,
can significantly affect gastrointestinal motility. Motility changes caused by these
agents might contribute to the development of IN. The aim of this experimental study
was to determine the preventive effects of indomethacin on lipopolysaccharide (LPS)-induced
IN in mice and to investigate the role of TNF and IL-6 on intussusception. Materials and Methods: Seventy-eight mice were divided into five groups. In the Control group (n = 6), no
procedure was done. In the Sham group (n = 6), 1 ml saline, in the Indomethacin group
(n = 6), 10 mg/kg of indomethacin, in the LPS group (n = 30), 12 mg/kg of LPS was
administered intraperitoneally (IP). In the Treatment group (n = 30), 10 mg/kg of
indomethacin was administered IP following 12 mg/kg of LPS. All animals were laparotomized
6 hours following IP injections. The existence of IN was noted and blood specimens
were obtained. TNFα and IL-6 plasma level measurements were performed by standard
ELISA for mice. The results were compared using the Mann-Whitney U test and one-way
ANOVA test. A value of p < 0.05 was considered significant. Results: Five mice (1 in the control, 2 in the LPS, 2 in the Treatment group) were excluded
from the study. IN was observed in 6 (20 %) mice in the LPS group, whereas it was
not found in any mice in the Treatment group. Mean TNFα and IL-6 levels were statistically
higher in the LPS group (394.72 ± 403.79; 195.18 ± 218.37 pg/ml, respectively) compared
to all other groups, including the Treatment group (p < 0.05 for each comparison).
Within the LPS group of mice, the levels were higher in animals with IN compared to
the mice without IN. Conclusion: Increased TNFα and IL-6 levels induced by LPS correlated well with the occurrence
of IN, and a decrease in these levels via cyclooxygenase (COX) inhibition by indomethacin
prevented IN from forming in this experimental model.
Key words
tumor necrosis factor (TNF) - interleukin - indomethacin - intussusception
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Dr. Ramazan Karabulut
Department of Pediatric Surgery
Gazi University
Faculty of Medicine
Besevler
06550 Ankara
Turkey
Email: karabulutr@yahoo.com
