Pre-Exercise Carbohydrate Status Influences Carbohydrate-Mediated Attenuation of Post-Exercise Cytokine Responses

A. J. Cox; D. B. Pyne; G. R. Cox; R. Callister; M. Gleeson

doi:10.1055/s-2008-1038753

International Journal of Sports Medicine, Inhaltsverzeichnis

Int J Sports Med 2008; 29(12): 1003-1009
DOI: 10.1055/s-2008-1038753

Immunology

Pre-Exercise Carbohydrate Status Influences Carbohydrate-Mediated Attenuation of Post-Exercise Cytokine Responses

A. J. Cox¹ ^, ² , D. B. Pyne³ , G. R. Cox⁴ , R. Callister² , M. Gleeson²

¹Department of Physiology, Australian Institute of Sport, Belconnen, Australia
²School of Biomedical Sciences, University of Newcastle, Newcastle, Australia
³Department of Physiology, Australian Institute of Sport, Canberra, Australia
⁴Department of Sports Nutrition, Australian Institute of Sport, Canberra, Australia

Abstract

Most studies investigating the effects of acute carbohydrate (CHO) ingestion on post-exercise cytokine responses have involved fasted athletes. This study characterised the effects of acute CHO beverage ingestion preceded by consumption of a CHO-containing pre-exercise meal. Sixteen highly-trained male cyclists/triathletes (age: 30.6 ± 5.6 y; V˙O_2max: 64.8 ± 4.7 ml · kg · min⁻¹ [mean ± SD]) undertook two cycle ergometry trials involving randomised consumption of a 10 % CHO beverage (15 mL · kg⁻¹ · hr⁻¹) or water (H₂O). Trials were undertaken 2 h after a breakfast providing 2.1 g CHO · kg⁻¹ body mass (BM) (48 kJ · kg⁻¹ BM) and consisted of 100 min steady state cycle ergometry at 70 % V˙O_2max followed by a time trial of ∼ 30 min duration. Blood samples were collected pre-, post- and 1 h post-exercise for measurement of Interleukin (IL)-6, IL-8, IL-10 and IL-1ra. Time-trial performance was not substantially different between CHO and H₂O trials (4.5 %, p = 0.42). Neither IL-6 nor IL-8 responses were substantially reduced in the CHO compared to the H₂O trial. There was a substantial reduction in IL-10 (32 %, p = 0.05) and IL-1ra (43 %, p = 0.02) responses at 1 h post-exercise with CHO compared to H₂O ingestion. In conclusion, the previously shown attenuating effects of CHO ingestion during exercise on cytokine responses appear reduced when athletes consume a CHO-containing pre-exercise meal.

Key words

immunity - carbohydrate - athlete - exercise

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References

1 Batterham A M, Hopkins W G. Making meaningful inferences about magnitudes. Int J Sports Physiol Perform. 2006; 1 50-57
2 Bishop N C, Walsh N P, Haines D L, Richards E E, Gleeson M. Pre-exercise carbohydrate status and immune responses to prolonged cycling: II. Effect on plasma cytokine concentration. Int J Sport Nutr Exerc Metab. 2001; 11 503-512
3 Davison G, Gleeson M. Influence of acute vitamin C and/or carbohydrate ingestion on hormonal, cytokine and immune responses to prolonged exercise. Int J Sport Nutr Exerc Metab. 2005; 15 465-479
4 Gannon G A, Rhind S G, Suzui M, Shek P N, Shephard R J. Circulating levels of peripheral blood leucocytes and cytokines following competitive cycling. Ca J Appl Physiol. 1997; 22 133-147
5 Hawley J A, Noakes T D. Peak power output predicts maximal oxygen uptake and performance time in trained cyclists. Eur J Appl Physiol. 1992; 65 79-83
6 Henson D A, Nieman D C, Blodgett A D, Butterworth D E, Utter A, Davis J M, Sonnenfeld G, Morton D S, Fagoaga O R, Nehlsen-Cannarella S L. Influence of exercise mode and carbohydrate on the immune response to prolonged exercise. Int J Sport Nutr. 1999; 9 213-228
7 Henson D A, Nieman D C, Nehlsen-Cannarella S L, Fagoaga O R, Shannon M, Bolton M R, Davis J M, Gaffney C T, Kelln W J, Austin M D, Hjertman J M, Schilling B K. Influence of carbohydrate on cytokine and phagocytic responses to 2 h of rowing. Med Sci Sports Exerc. 2000; 32 1384-1389
8 Henson D A, Nieman D C, Parker J C, Rainwater M K, Butterworth D E, Warren B J, Utter A, Davis J M, Fagoaga O R, Nehlsen-Cannarella S L. Carbohydrate supplementation and the lymphocyte proliferative response to long endurance running. Int J Sports Med. 1998; 19 574-580
9 Henson D A, Nieman D C, Pistilli E E, Schilling B, Colacino A R, Utter A C, Fagoaga O R, Vinci D M, Nehlsen-Cannarella S L. Influence of carbohydrate and age on lymphocyte function following a marathon. Int J Sport Nutr Exerc Metab. 2004; 14 302-316
10 Jeukendrup A E, Borghouts L B, Saris W H, Wagenmakers A J. Reduced oxidation rates of ingested glucose during prolonged exercise with low endogenous CHO availability. J Appl Physiol. 1996; 81 1952-1957
11 Keller C, Keller P, Marshal S, Pedersen B K. IL‐6 gene expression in human adipose tissue in response to exercise – Effect of carbohydrate ingestion. J Physiol. 2003; 550 927-931
12 Keller C, Steensberg A, PIlegaard H, Osada T, Saltin B, Pedersen B K, Neufer P D. Transcriptional activation of the IL‐6 gene in human contracting skeletal muscle: Influence of muscle glycogen content. FASEB J. 2001; 15 2748-2750
13 Lancaster G I, Jentjens R L, Moseley L, Jeukendrup A E, Gleeson M. Effect of pre-exercise carbohydrate ingestion on plasma cytokine, stress hormone, and neutrophil degranulation responses to continuous, high-intensity exercise. Int J Sport Nutr Exerc Metab. 2003; 13 436-453
14 Lancaster G I, Khan Q, Drysdale P T, Wallace F, Jeukendrup A E, Drayson M T, Gleeson M. Effect of prolonged exercise and carbohydrate ingestion on type 1 and type 2 T lymphocyte distribution and intracellular cytokine production in humans. J Appl Physiol. 2005; 98 565-571
15 Li T, Gleeson M. The effects of carbohydrate supplementation during the second of two prolonged cycling bouts on immunoendocrine responses. Eur J Appl Physiol. 2005; 95 391-399
16 Nehlsen-Cannarella S L, Fagoaga O R, Nieman D C, Henson D A, Butterworth D E, Schmitt R L, Bailey E M, Warren B J, Utter A, Davis J M. Carbohydrate and the cytokine response to 2.5 h of running. J Appl Physiol. 1997; 82 1662-1667
17 Nieman D C, David J M, Brown V A, Henson D A, Dumke C L, Utter A C, Vinci D M, Downs M F, Smith J C, Carson J, Brown A, McAnulty S R, McAnulty L S. Influence of carbohydrate ingestion on immune changes after 2 h of intensive resistance training. J Appl Physiol. 2004; 96 1292-1298
18 Nieman D C, Davis J M, Henson D A, Gross S J, Dumke C L, Utter A C, Vinci D M, Carson J A, Brown A, McAnulty S R, McAnulty L S, Triplett N T. Muscle cytokine mRNA changes after 2.5 h of cycling: Influence of carbohydrate. Med Sci Sports Exerc. 2005; 37 1283-1290
19 Nieman D C, Davis J M, Walberg-Rankin J, Shute M, Dumke C L, Utter A C, Vinci D M, Carson J A, Brown A, Lee W J, McAnulty S R, McAnulty L S. Carbohydrate ingestion influences skeletal muscle cytokine mRNA and plasma cytokine levels after a 3-h run. J Appl Physiol. 2003; 94 1917-1925
20 Nieman D C, Henson D A, Davis M J, Dumke C L, Utter A C, Murphy A, Pearce S, Gojanovich G, McAnulty S R, McAnulty L. Blood leukocyte mRNA expression for IL‐10, IL‐1ra, and IL‐8, but not IL‐6, increases post-exercise. Int J Interferon Cytokine Res. 2006; 26 668-674
21 Nieman D C, Henson D A, Smith L L, Utter A C, Vinci D M, Davis J M, Kaminsky D E, Shute M. Cytokine changes after a marathon race. J Appl Physiol. 2001; 91 109-114
22 Nieman D C, Nehlsen-Cannarella S L, Fagoaga O R, Henson D A, Utter A, Davis M J, Williams F, Butterworth D E. Influence of mode and carbohydrate on the cytokine response to heavy exertion. Med Sci Sports Exerc. 1998; 30 671-678
23 Pedersen B K, Steensberg A, Schjerling P. Muscle-derived interleukin-6: Possible biological effects. J Physiol. 2001; 536 329-337
24 Scharhag J, Meyer T, Auracher M, Gabriel H, Kindermann W. Effects of graded carbohydrate supplementation on the immune response in cycling. Med Sci Sports Exerc. 2006; 38 286-292
25 Shephard R J. Regression to the mean. A threat to exercise science?. Sports Med. 2003; 33 575-584
26 Starkie R L, Angus D J, Rolland J, Hargraves M, Febbraio M A. Effect of prolonged, submaximal exercise and carbohydrate ingestion on monocyte intracellular cytokine production in humans. J Physiol. 2000; 528 647-655
27 Starkie R L, Arkinstall M J, Koukoulas I, Hawley J A, Febbraio M A. Carbohydrate ingestion attenuates the increase in plasma interleukin-6, but not skeletal muscle interleukin-6 mRNA, during exercise in humans. J Physiol. 2001; 533 585-591
28 Steensberg A, Febbraio M A, Osada T, Schjerling P, vanHall G, Saltin B, Pedersen B K. Interleukin-6 production in contracting human skeletal muscle is influenced by pre-exercise muscle glycogen content. J Physiol. 2001; 537 633-639
29 Steensberg A, Fischer C P, Keller C, Moller K, Pedersen B K. IL‐6 enhances plasma IL‐1ra, IL‐10 and cortisol in humans. Am J Physiol. 2003; 285 E422-E437
30 Steensberg A, vanHall G, Osada T, Sacchetti M, Saltin B, Pedersen B K. Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6. J Physiol. 2000; 529 237-242
31 Timmons B W, Tarnopolsky M A, Bar-or O. Immune responses to strenuous exercise and carbohydrate intake in boys and men. Pediatr Res. 2004; 56 227-234
32 Tsintzas O K, Williams C, Singh R, Wilson W, Burrin J. Influence of carbohydrate-electrolyte drinks on marathon running performance. Eur J Appl Physiol. 1995; 70 154-160
33 vanHall G, Steensberg A, Sacchetti M, Fischer C, Keller C, Schjerling P, Hiscock N, Moller K, Saltin B, Febbraio M A, Pedersen B K. Interleukin-6 stimulates lipolysis and fat oxidation in humans. J Clin Endocrinol Metab. 2003; 88 3005-3010

Amanda Cox

Australian Institute of Sport
Department of Physiology

PO Box 176

Belconnen 2616

Australia

eMail: amanda.cox@newcastle.edu.au