Horm Metab Res 2008; 40(7): 459-466
DOI: 10.1055/s-2008-1065318
Animals, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Induction and Reversibility of Insulin Resistance in Rats Exposed to Exogenous D-Fructose

J. Cancelas 1 , P. G. Prieto 1 , M. García-Arévalo 1 , V. Sancho 1 , M. L. Villanueva-Peñacarrillo 1 , W. J. Malaisse 2 , I. Valverde 1
  • 1Department of Metabolism, Nutrition and Hormones, Fundacíón Jiménez Díaz, Madrid, Spain
  • 2Laboratory of Experimental Hormonology, Brussels Free University, Brussels, Belgium
Further Information

Publication History

received 23.04.2007

accepted 06.11.2007

Publication Date:
07 April 2008 (online)

Abstract

Long-term exposure of normal rats to a fructose-enriched diet or drinking water is currently used as an animal model for experimental insulin resistance. The present study deals with a comparison between rats given access to either a fructose-enriched diet or fructose-enriched drinking water. In both situations, a decrease in food intake and body weight gain, and the induction of insulin resistance with intolerance to D-glucose despite increased secretory response to the aldohexose of insulin-producing cells were documented. Moreover, the rats exposed to exogenous D-fructose displayed a lesser sensitivity to overnight fasting than control animals, in terms of the alteration of glucose homeostasis and reduction of the ratio between plasma insulin and D-glucose concentration. It is also shown that the fructose-induced insulin resistance, as assessed in a hyperinsulinemic-euglycemic clamp, represents a phenomenon reversed within 15–30 days after removal of the keto-hexose from the drinking water.

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Correspondence

I. ValverdeMD, PhD 

Metabolismo Nutrición y Hormonas

Fundación Jiménez Díaz

Avda. Reyes Católicos 2

28040 Madrid

Spain

Phone: +34/915/50 48 99

Fax: +34/915/44 02 47

Email: ivalverde@fjd.es

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